Research Article

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09/27/2017
Ecuador; Gene frequency; Humans; Microsatellite Repeats; Polymorphism, Genetic; Population

One hundred and eighty-two samples of unrelated people who requested the paternity test at the Molecular Biology and Genetics Laboratory of the Catholic University of Cuenca-Ecuador in the province of Azuay were studied, except for the D1S1656 (180 samples) and SE33 (89 samples) markers. The STRs D22S1045, D3S1358, VWA, D16S539, D2S1338, D8S1179, D21S11, D18S51, D19S433, TH01, FGA, D1S1656, ... more

P.P. Orellana; C.F. Andrade; C.L. Arciniegas; G.C. Iannacone
12/02/2016
Animal Shells; Animals; Bivalvia; Breeding; Evolution, Molecular; Female; Fresh Water; Genetic variation; Genetics, Population; male; Microsatellite Repeats; Phenotype; Phylogeny; Quantitative trait loci

Four Hyriopsis cumingii populations, a breeding population (BP), a cultured population (FP), two wild populations from Poyang Lake (PY) and Dongting Lake (DT), and an H. schlegelii population were collected (JX), and the first filial generations (F1) were bred synchronously. The shell nacre polymorphisms, population genetic diversity, and genetic structures of the F1 of each population were ... more

L.M. Wu; Z.Y. Bai; X.J. Liu; C. Jin; H. Yin; J.L. Li
12/02/2016
Animals; Body weight; Cattle; Genome-wide association study; Genotype; Models, Biological; Phenotype; Polymorphism, Single Nucleotide; Quantitative trait loci

This study aimed to investigate the predictability of a phenotype when using a dynamic model of cattle growth. Genotypic and phenotypic information on Nellore (Bos indicus) cattle were used in a genome-wide association analysis designed to contrast the biological interpretation of core parameters [conversion efficiency of metabolizable energy to net energy for gain (k) and adjusted final ... more

M.C. Freua; M.H.A. Santana; R.V. Ventura; J.B.S. Ferraz
12/02/2016
Animals; Brazil; Breeding; Cattle; Gene frequency; Genetic markers; Leptin; Polymorphism, Single Nucleotide; Promoter Regions, Genetic; Red Meat; Sequence Analysis, DNA

Brazil is the world's largest producer of beef cattle; however, the quality of its herds needs to be improved. The use of molecular markers as auxiliary tools in selecting animals for reproduction with high pattern for beef production would significantly improve the quality of the final beef product in Brazil. The leptin gene has been demonstrated to be an excellent candidate gene for bovine ... more

R.C. Guimarães; J.S.N. Azevedo; S.C. Corrêa; J.E.G. Campelo; E.M. Barbosa; E.C. Gonçalves; S. Filho
12/02/2016
Animals; Bayes Theorem; Biological evolution; Butterflies; Evolution, Molecular; Gene order; Genome, Insect; Genome, Mitochondrial; Likelihood Functions; Phylogeny

Pieridae is a butterfly family whose evolutionary history is poorly understood. Due to the difficulties in identifying morphological synapomorphies within the group and the scarcity of the fossil records, only a few studies on higher phylogeny of Pieridae have been reported to date. In this study, we describe the complete mitochondrial genomes of four pierid butterfly species (Aporia martineti ... more

Y. Cao; J.S. Hao; X.Y. Sun; B. Zheng; Q. Yang
12/02/2016
Animals; Avian Proteins; Chickens; Female; Genetic association studies; Genotype; Insulin-like growth factor I; Phenotype; Polymorphism, Single-Stranded Conformational; Quantitative trait loci; Reproduction; Selection, Genetic

The insulin-like growth factor, IGF-I, plays an important role in the development of growth and reproductive traits. Single-strand conformation polymorphism analysis was used to detect and analyze polymorphisms and expression profiles of the IGF-I gene and its association with growth and reproductive traits of Jinghai yellow chickens. A point mutation g.295T>C was detected in the IGF-I gene ... more

M.A. Abdalhag; T. Li; L. Duan; T. Zhang; G. Zhang; J. Wang; Y. Wang
12/19/2016
Animals; Antimutagenic Agents; Antioxidants; Chromosome aberrations; Cyclophosphamide; Fruit; male; Malpighiaceae; Models, Animal; Plant Extracts; Rats; Rats, Wistar

Barbados cherry (BC) (Malpighia glabra Linnaeus) is a functional fruit that is consumed to prevent disease. It is used as an adjuvant in the treatment of several diseases, and acts as an antianemic, an appetite stimulant, a wound healer, an anti-inflammatory, a mineralizer, an antifungal, and an antioxidant agent. Several chemotherapeutic agents, such as cyclophosphamide, may result in ... more

E. Düsman; I.V. Almeida; L.T.D. Tonin; V.E.P. Vicentini
12/19/2016
Animals; Feces; Foxes; Genetic markers; Genotyping Techniques; Heterozygote; Microsatellite Repeats

The Ezo red fox (Vulpes vulpes schrencki), a subspecies endemic to Hokkaido island, Japan, is a known host species for the tapeworm Echinococcus multilocularis. To develop tools for molecular ecological studies, we isolated 28 microsatellite regions from the genome of Ezo red fox, and developed 18 polymorphic microsatellite markers. These markers were characterized using 7 individuals and 22 ... more

T. Tada; Y. Seki; Y. Kameyama; Y. Kikkawa; K. Wada
12/19/2016
Animals; Female; Forkhead Box Protein L2; Forkhead Transcription Factors; Gene expression; Goats; Hair follicle; Quantitative trait loci; Real-time polymerase chain reaction; Toll-Like Receptors; Wnt4 Protein

The mechanism of dominant follicle selection is unclear because of its physiological complexity. However, some studies have reported that the immune system plays an important role in reproductive physiology. The objective of the current study was to investigate the differential expression of Toll-like receptors (TLRs) in the dominant (DFs) and nondominant follicles (NFs), and to determine the ... more

G.X. E; Y.J. Zhao; Y.H. Ma; M.X. Chu; Q.H. Hong; S.J. Lv; L. Zhu; F.K. Li; Y.G. Han; X. Wu; Y.F. Huang
12/19/2016
Animals; Cattle; Embryo Culture Techniques; Female; Gene Expression Regulation, Developmental; GRB10 Adaptor Protein; Point mutation; Polymorphism, Single Nucleotide; Quantitative trait loci; Sequence Analysis, DNA; Superovulation

The application of assisted reproductive technology in animal production benefits the economy and conservation of biological resources. Single nucleotide polymorphism (SNPs) was used as predictive markers for breeding and reproduction. In the present study, we examined the association between a SNP of the grb10 gene and superovulation traits in cattle. Sequencing results indicated a point ... more

Y. Wu; Z. Zhang; J.B. Zhang; Q. Deng

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