Research Article

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12/19/2016
Animals; Feces; Foxes; Genetic markers; Genotyping Techniques; Heterozygote; Microsatellite Repeats

The Ezo red fox (Vulpes vulpes schrencki), a subspecies endemic to Hokkaido island, Japan, is a known host species for the tapeworm Echinococcus multilocularis. To develop tools for molecular ecological studies, we isolated 28 microsatellite regions from the genome of Ezo red fox, and developed 18 polymorphic microsatellite markers. These markers were characterized using 7 individuals and 22 ... more

T. Tada; Y. Seki; Y. Kameyama; Y. Kikkawa; K. Wada
12/19/2016
Animals; Female; Forkhead Box Protein L2; Forkhead Transcription Factors; Gene expression; Goats; Hair follicle; Quantitative trait loci; Real-time polymerase chain reaction; Toll-Like Receptors; Wnt4 Protein

The mechanism of dominant follicle selection is unclear because of its physiological complexity. However, some studies have reported that the immune system plays an important role in reproductive physiology. The objective of the current study was to investigate the differential expression of Toll-like receptors (TLRs) in the dominant (DFs) and nondominant follicles (NFs), and to determine the ... more

G.X. E; Y.J. Zhao; Y.H. Ma; M.X. Chu; Q.H. Hong; S.J. Lv; L. Zhu; F.K. Li; Y.G. Han; X. Wu; Y.F. Huang
2016 Dec 19
Animals; Astragalus propinquus; Biomarkers; Bone Density; Disease Models, Animal; Dose-Response Relationship, Drug; Drugs, Chinese Herbal; Female; Humans; Mice; Osteoporosis, Postmenopausal; Ovariectomy; Polysaccharides; Random Allocation; Signal transduction

Postmenopausal osteoporosis, a common type of osteoporosis in women, has become a serious public health issue. Astragalus polysaccharides (APS), possessing various pharmacological activities, are the active ingredients of Radix Astragali. It can be advantageous in the treatment of postmenopausal osteoporosis. In the present study, we evaluated the potential therapeutic effects of APS on ... more

J. Huo; X. Sun
12/19/2016
Animals; Cattle; Embryo Culture Techniques; Female; Gene Expression Regulation, Developmental; GRB10 Adaptor Protein; Point mutation; Polymorphism, Single Nucleotide; Quantitative trait loci; Sequence Analysis, DNA; Superovulation

The application of assisted reproductive technology in animal production benefits the economy and conservation of biological resources. Single nucleotide polymorphism (SNPs) was used as predictive markers for breeding and reproduction. In the present study, we examined the association between a SNP of the grb10 gene and superovulation traits in cattle. Sequencing results indicated a point ... more

Y. Wu; Z. Zhang; J.B. Zhang; Q. Deng
12/19/2016
Animals; Animals, Genetically Modified; Apoptosis Regulatory Proteins; Crosses, Genetic; Drosophila melanogaster; Drosophila Proteins; Gene Expression Regulation, Developmental; Gene Knockdown Techniques; Humans; male; Membrane Proteins; Neurons; Proto-Oncogene Proteins c-bcl-2; Sequence Homology, Amino Acid; Transgenes

Lifeguard is an integral transmembrane protein that modulates FasL-mediated apoptosis by interfering with the activation of caspase 8. It is evolutionarily conserved, with homologues present in plants, nematodes, zebra fish, frog, chicken, mouse, monkey, and human. The Lifeguard homologue in Drosophila, CG3814, contains the Bax inhibitor-1 family motif of unknown function. Downregulation of ... more

P.G. M'Angale; B.E. Staveley
12/19/2016
3' Untranslated Regions; Animals; Cell differentiation; Cell proliferation; Cells, Cultured; Down-regulation; Gene Expression Regulation, Developmental; Microfilament Proteins; MicroRNAs; Muscle development; Muscle Proteins; Myoblasts; Swine

MicroRNAs (miRNAs) play important roles in the development and biochemical functions of skeletal muscles. However, targeting of miRNAs to structural genes involved in Z-discs have not been investigated. Here, we describe a highly expressed miRNA, miR-432, in pig embryonic skeletal muscle, which appeared to target myozenin1 (MYOZ1), a protein involved in the muscular sarcomere microstructure. ... more

R.M. Ren; H. Liu; S.H. Zhao; J.H. Cao
12/19/2016
Animals; Antimutagenic Agents; Cell line; Cell proliferation; DNA damage; Dose-Response Relationship, Drug; Plant Extracts; Rats; Vanillic Acid

The consumption of healthy and natural foods has increased over the last few years, primarily because these foods are rich in substances with biological properties of interest, such as exerting anticancer effects and decreasing oxidative stress in living tissues. These foods support adequate nutrition, maintain health, and improve quality of life. Vanillic acid (VA) is a phenolic compound used ... more

I.V. Almeida; F.M.L. Cavalcante; V.E.P. Vicentini
12/23/2016
Animals; DNA, Ribosomal; Fishes; In Situ Hybridization, Fluorescence; Karyotype; Karyotyping; Nucleolus organizer region

The spotted knifejaw, Oplegnathus punctatus, is an important aquaculture fish species in China. To better understand the chromosomal microstructure and the karyotypic origin of this species, cytogenetic analysis was performed using Giemsa staining to identify metaphase chromosomes, C-banding to detect C-positive heterochromatin, silver staining to identify the nucleolus organizer regions (Ag- ... more

P.Z. Li; D.D. Cao; X.B. Liu; Y.J. Wang; H.Y. Yu; X.J. Li; Q.Q. Zhang; X.B. Wang
12/23/2016
Actins; Animals; Fishes; Genetic markers; Real-time polymerase chain reaction; Reference Standards; RNA, Ribosomal, 18S

Tambaqui, Colossoma macropomum, is the main native freshwater fish in Brazilian aquaculture. Therefore, intensive research pressure has been applied to the species to support new technologies for tambaqui farming. Molecular biology represents a tool that can be used to investigate every field of applied biology, from fish physiology to the effects of climate change. Based on the importance of ... more

A.R. Nascimento; G.F. Silva; G.F. Gualberto; F.L. Almeida
12/23/2016
Animals; Cloning, Molecular; Disease resistance; Flavonoids; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Plant; Genes, Plant; Insecta; Metabolic Networks and Pathways; Pinus; Plant Diseases

Pinus massoniana Lamb. is an important timber and turpentine-producing tree species in China. Dendrolimus punctatus and Dasychira axutha are leaf-eating pests that have harmful effects on P. massoniana production. Few studies have focused on the molecular mechanisms underlying pest resistance in P. massoniana. Based on sequencing analysis of the transcriptomes of insect-resistant P. massoniana ... more

Z.Q. Yang; H. Chen; J.H. Tan; H.L. Xu; J. Jia; Y.H. Feng

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