Research Article

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2017 Aug 17
alpha-Amylases; Animal Husbandry; Animals; Chickens; Diet; Dietary Supplements; Digestion; Energy metabolism; male

This study aimed to evaluate the supplementation levels of an exogenous alpha-amylase in broilers diets and compare two indicators in determining the diets energy. The experiment was divided into two parallel evaluations, being one of performance and the other of metabolism. In performance assay, 1,700 one-day-old Cobb-500 male chicks were used. The animals were distributed in 50 experimental ... more

H.B. Oliveira; M.I.A. Silva; F.R. Mesquita
2017 Feb 08
Animals; Calcium; Calcium-Binding Proteins; Cation Transport Proteins; Drosophila; Drosophila melanogaster; Gene expression; Locomotion; Mitochondria; Mitochondrial Membrane Transport Proteins; Neurons; Proto-Oncogene Proteins c-bcl-2

The mitochondrial calcium uptake 1 (MICU1) is a regulatory subunit of the mitochondrial calcium uniporter that plays an important role in calcium sensing. It contains two EF-hand domains that are well conserved across diverse species from protozoa to plants and metazoans. The loss of MICU1 function in mammals is attributed to several neurological disorders that involve movement dysfunction. ... more

P.G. M'Angale; B.E. Staveley
2017 Apr 20
Animals; Cetacea; DNA Barcoding, Taxonomic; Electron Transport Complex IV; Endangered species; Genetic markers; Reference Standards; Sequence alignment

Molecular identification is very useful in cases where morphology-based species identification is not possible. Examples for its application in cetaceans include the identification of carcasses of stranded animals in advanced state of decomposition and body parts that are illegally traded. One DNA region that is often used for molecular identification is the Folmer region of the mitochondrial ... more

L.H.O. Falcão; A.S. Campos; J.E.P. Freitas; M.A.A. Furtado-Neto; V.V. Faria
2017 Aug 17
Animals; Diptera; DNA, Mitochondrial; Electron Transport Complex IV; Insect Proteins; Paraguay; Soybeans

We provided the first scientific record of Melanagromyza sojae (Zehntner, 1900), through molecular characterization of partial mtDNA COI gene, that confirms the occurrence of this pest in Paraguay. Previously reported in Brazil, an outbreak of larvae of M. sojae known as the soybean stem fly (SSF) that belongs to the family Agromyzidae, was also noted in soybean fields from the Canindeyú, Alto ... more

J.V.C. Guedes; J.A. Arnemann; L.E. Curioletti; L.M. Burtet; M.L. Ramírez-Paredes; D. Noschang; I. de Oliveira; W.T. Tay
Melatonin; Mitochondria; Myocardial ischemia/reperfusion injury

We aimed to evaluate the effect of melatonin on myo­cardial cell oncosis in the myocardial ischemia/reperfusion injury rat, and the role of the mitochondrial permeability transition pore (MPTP) therein. Sprague Dawley rats (N = 60) were randomly divided into five groups of 12 rats each: control, ischemia/reperfusion (I/R), melatonin treatment (MT), melatonin treatment + atractyloside (MT ... more

L.F. Liu; Z.H. Qian; Q. Qin; M. Shi; H. Zhang; X.M. Tao; W.P. Zhu
Hypertension; Mitochondria; Mitochondrial tRNA mutations; Pathogenic mutations

Mutations in mitochondrial DNA have been found to be associated with hypertension. Of these, mitochondrial transfer RNA (mt-tRNA) is a hot spot for these pathogenic mutations. It is generally believed that these mutations may result in the failure of mt-tRNA metabolism, thereby worsening mitochondrial dysfunction and resulting in hypertension. mt-tRNA is known for its high frequency of ... more

S.L. Yin; C. Lan; H. Pei; Z.Q. Zhu
Glycolytic potential; meat quality; Mitochondria; Muscle fiber type; Tibetan pigs

The myosin heavy chain (MyHC) composition, glycolytic potential, mitochondrial content, and gene expression related to energy metabolism were analyzed in eight muscles from Tibetan pigs, to study how meat quality develops in different muscle tissues. The muscles were classified into three clusters, based on MyHC composition: masseter, trapezius, and latissimus dorsi ... more

L.Y. Shen; J. Luo; H.G. Lei; Y.Z. Jiang; L. Bai; M.Z. Li; G.Q. Tang; X.W. Li; S.H. Zhang; L. Zhu
Chloroplast; Genetic relationship; Mitochondria; Nucleus; Prickly pear; Xoconostle

Molecular methods are powerful tools in characterizing and determining relationships between plants. The aim of this study was to study genetic divergence between 103 accessions of Mexican Opuntia. To accomplish this, polymerase chain reaction (PCR)-restriction fragment length polymorphism analysis of three chloroplast intergenic spacers (atpB-rbcL, trnL ... more

S. Samah; E. Valadez-Moctezuma; K.S. Peláez-Luna; S. Morales-Manzano; P. Meza-Carrera; R.C. Cid-Contreras; S. Samah; E. Valadez-Moctezuma; K.S. Peláez-Luna; S. Morales-Manzano; P. Meza-Carrera; R.C. Cid-Contreras
Electron transport chain; Energy metabolism; Gene expression; Oxidative phosphorylation

This study was carry out to evaluate mRNA expression of mitochondrial cytochrome c oxidase III in the Pectoralis superficialis muscle of 28-day-old quails fed diets containing 0, 8, and 12% glycerol. Total RNA was extracted (N = 10) and cDNA was amplified using specifics primers for qRT-PCR. Feed efficiency and feed intake were evaluated. COX III mRNA expression in breast muscle was ... more

S.C.C. Silva; E. Gasparino; E. Batista; F. Tanamati; A.P.D. Vesco; B. Lala; D.P. de Oliveira; S.C.C. Silva; E. Gasparino; E. Batista; F. Tanamati; A.P.D. Vesco; B. Lala; D.P. de Oliveira
2016 Dec 02
Animal Shells; Animals; Bivalvia; Breeding; Evolution, Molecular; Female; Fresh Water; Genetic variation; Genetics, Population; male; Microsatellite Repeats; Phenotype; Phylogeny; Quantitative trait loci

Four Hyriopsis cumingii populations, a breeding population (BP), a cultured population (FP), two wild populations from Poyang Lake (PY) and Dongting Lake (DT), and an H. schlegelii population were collected (JX), and the first filial generations (F1) were bred synchronously. The shell nacre polymorphisms, population genetic diversity, and genetic structures of the F1 of each population were ... more

L.M. Wu; Z.Y. Bai; X.J. Liu; C. Jin; H. Yin; J.L. Li