Research Article

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Chronic heart failure; Genotype; Glu298Asp; NOS3 gene; Polymorphism

The eNOS Glu298Asp (rs1799983) polymorphism of the NOS3 gene has been implicated as a risk factor for cardiovascular diseases; however, not all studies find significant associations. We examined this possibility in a Russian (Siberian) population. One hundred patients with chronic heart failure and 40 controls were investigated. PCR analysis was performed ... more

E.S. Goppe; P.E. Maltese; A. Chernova; S.Y. Nikulina; A. Semenchukov; A. Matveeva; E. Manara; M. Bertelli
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Endometriosis; eNOS; Genotype; Infertility; Polymorphism

Endometriosis is a chronic disease with adverse implications for fertility. We investigated the G894T polymorphism in the endothelial nitric oxide synthase gene in fertile and infertile women with endometriosis. This prospective study included a group of 52 women with a laparoscopic diagnosis of endometriosis. DNA was extracted from blood obtained from 47 patients ... more

R.C.P.Ce Silva; L.C. de Faria; K.S.Fe Silva; K.K.V.O. Moura; C.L.Ribeiro Júnior; L.A. Guillo
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Genetic diversity; Genetic variation; Mutagenesis; Mutation breeding; Oryza sativa

We studied the genetic variability due to mutation induced by γ-rays (10, 15, and 20 Kr) on various traits of twelve rice genotypes. Mutated and non-mutated seeds were sown in the field between July 2013 and 2014 using a split plot design. Yield and yield-related trait data was recorded, which showed significant (P < 0.05) genotypic and irradiation effects. Gamma radiation exerted non- ... more

M. Naeem; F. Ghouri; M.Q. Shahid; M. Iqbal; F.S. Baloch; L. Chen; S. Allah; M. Babar; M. Rana
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Eggplant; Genetic variation; Phomopsis blight; Random amplified polymorphic DNA; Resistance

Disease susceptibility and genetic variability in 10 eggplant genotypes were studied after inoculating Phomopsis vexans under confined field conditions. Random amplified polymorphic DNA (RAPD) markers were used to assess genetic variation and relationships among eggplant genotypes. The disease index of leaves ranged 0.208-13.79%, while fruit infection ranged 2.15-42.76%. Two varieties ... more

H.A. Asad; M.B. Meah; S.N. Begum; M.I. Khalil; M.Y. Rafii; M.A. Latif
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Chinese mitten crab; Ecdysteroid-regulated protein gene; Eriocheir sinensis; Genetic variation; MIH gene; UTRs

Crab grows by periodic molting, which is controlled by molt-inhibiting hormone (MIH) and ecdysteroids. Untranslated regions (UTRs) play crucial roles in the posttranscriptional regulation of gene expression. In this study, using crab collected from Changjiang (Yangtze), Huanghe (Yellow), Liaohe, and Yalujiang rivers, 33 haplotypes of the 3ꞌ-UTR of ecdysteroid-regulated protein (ERP ... more

X.G. Gao; H.S. Pu; C.B. He; X.B. Bao; W.D. Liu
01/08/2016
Allopolyploidization; Genetic variation; Qinghai-Tibet Plateau; Resynthesized Brassica napus

This study aimed to reveal the genetic and epigenetic variations involved in a resynthesized Brassica napus (AACC) generated from a hybridization between a B. rapa (AA) landrace and B. alboglabra (CC). Amplified fragment length polymorphism (AFLP), methylation-sensitive amplified polymorphism, and the cDNA-AFLP technique were performed to detect changes between ... more

H.D. Liu; Z.G. Zhao; D.Z. Du; C.R. Deng; G. Fu; H.D. Liu; Z.G. Zhao; D.Z. Du; C.R. Deng; G. Fu; H.D. Liu; Z.G. Zhao; D.Z. Du; C.R. Deng; G. Fu
12/19/2016
Arecaceae; Genetics, Population; Polymorphism, Genetic; Population Density; Seedlings; Waxes

The objective of this study was to assess the spatial genetic structure (SGS) at different life stages (cohorts) in a remnant population (N = 101) of Copernicia prunifera in the semiarid region of northeastern Brazil. Using seven inter-simple sequence repeat molecular markers, we were able to analyze 93 loci with 100% polymorphism. Seedlings had the highest level of genetic diversity (H = 0. ... more

F.A. Vieira; R.F. Sousa; C.G. Fajardo; M.M. Brandão
3/16/2017
Animals; China; DNA, Mitochondrial; Evolution, Molecular; Fishes; Genetics, Population; Haplotypes; Lakes; Phylogeny; Polymorphism, Genetic

Coilia ectenes is a commercially important fishery species in China. C. ectenes taihuensis is an endemic and dominant species found in Taihu Lake of China. When compared with C. ectenes, C. ectenes taihuensis lacks anadromous behavior, and can independently grow and reproduce in Taihu Lake. In this study, the mitochondrial DNA control region (D-loop) sequences were employed to investigate the ... more

J.J. Zhang; J.R. Duan; Y.F. Zhou; J.Y. Peng; D.A. Fang
12/19/2016
Animals; Feces; Foxes; Genetic markers; Genotyping Techniques; Heterozygote; Microsatellite Repeats

The Ezo red fox (Vulpes vulpes schrencki), a subspecies endemic to Hokkaido island, Japan, is a known host species for the tapeworm Echinococcus multilocularis. To develop tools for molecular ecological studies, we isolated 28 microsatellite regions from the genome of Ezo red fox, and developed 18 polymorphic microsatellite markers. These markers were characterized using 7 individuals and 22 ... more

T. Tada; Y. Seki; Y. Kameyama; Y. Kikkawa; K. Wada
1/23/2017
Breeding; Crosses, Genetic; Disease resistance; Genes, Plant; Genetic markers; Microsatellite Repeats; Oryza; Plant Diseases

Rice (Oryza sativa L.) blast disease is one of the most destructive rice diseases in the world. The fungal pathogen, Magnaporthe oryzae, is the causal agent of rice blast disease. Development of resistant cultivars is the most preferred method to achieve sustainable rice production. However, the effectiveness of resistant cultivars is hindered by the genetic plasticity of the pathogen genome. ... more

N. Hasan; M.Y. Rafii; A. Rahim; S.A. Nusaibah; N. Mazlan; S. Abdullah

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