Research Article

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2/08/2017
Animals; Bayes Theorem; Brazil; Evolution, Molecular; Fishes; Genetic variation; Genetics, Population; Microsatellite Repeats

Genetic variability is one of the important criteria for species conservation decisions. This study aimed to analyze the genetic diversity and the population differentiation of two natural populations of Arapaima gigas, a species with a long history of being commercially exploited. We collected 87 samples of A. gigas from Grande Curuai Lake and Paru Lake, located in the Lower Amazon region of ... more

P.F. Fazzi-Gomes; N. Melo; G. Palheta; S. Guerreiro; M. Amador; A.K. Ribeiro-Dos-Santos; S. Santos; I. Hamoy
2/16/2017
Bayes Theorem; Casearia; Chloroplasts; Conservation of Natural Resources; Discriminant analysis; DNA, Chloroplast; Genetic variation; Genetics, Population; Haplotypes; Microsatellite Repeats

Casearia grandiflora (Salicaceae) is a typical Cerrado species adapted to disturbed environments, making it useful for restoration projects. Knowledge of genetic diversity is important for establishing conservation strategies for this species. This study aimed to compare chloroplast haplotype diversity and structure of C. grandiflora, under the assumption that protected areas hold greater ... more

M.F. Costa; A.A. Pereira; J.B. Pinheiro; M.I. Zucchi; A.S.F. Araújo; R.L.F. Gomes; S.E.S. Valente; M.E.A. Oliveira; A.C.A. Lopes
09/27/2017
Analysis of variance; Bertholletia; Dimensional Measurement Accuracy; Fruit; Genetic variation; Genotype; Phenotype; Plant breeding; Principal component analysis; Quantitative Trait, Heritable

Repeatability studies on fruit species are of great importance to identify the minimum number of measurements necessary to accurately select superior genotypes. This study aimed to identify the most efficient method to estimate the repeatability coefficient (r) and predict the minimum number of measurements needed for a more accurate evaluation of Brazil nut tree (Bertholletia excelsa) ... more

A.B. Baldoni; H. Tonini; F.D. Tardin; S.C.C. Botelho; P.E. Teodoro
12/19/2016
cluster analysis; Cold Temperature; DNA, Plant; Genetic markers; Microsatellite Repeats; Polymorphism, Genetic; Stress, Physiological; Zea mays

In this study, eight inbred maize lines with high or low tolerance to cold temperature were investigated using bulk segregant analysis (BSA). Genetic diversity and genetic relationships were investigated using 100 simple sequence repeat (SSR) markers linked to cold tolerance in maize. A total of 319 alleles were identified. Among these, 128 were high coldtolerant-specific alleles and 61 were ... more

M.Q.U. Farooqi; K.J. Sa; T.K. Hong; J.K. Lee
2/08/2017
cluster analysis; Disease resistance; Genetic variation; Genotype; Gossypium; Phenotype; Plant Diseases; Quantitative Trait, Heritable

In Pakistan, cotton crop has been under enormous threat of cotton leaf curl disease (CLCuD) over the last four decades. In order to estimate genetic diversity in cotton germplasm CLCuD resistance, we assessed 100 cotton genotypes for their CLCuD resistance/tolerance and other related agronomical traits. Various statistical analytical tools, including correlation analysis, cluster analysis, and ... more

M. Javed; S.B. Hussain; M. Baber
3/30/2017
Alleles; Animals; Brazil; Breeding; Chromosome Mapping; DNA; Genetic Drift; Genetic variation; Genetics, Population; Genotype; Goats; Heterozygote; Microsatellite Repeats

The aim of this study was to access the genetic diversity and relatedness between Canindé and British Alpine goat breeds in the States of Piauí and Ceará using microsatellite markers. Genomic DNA was isolated from hair samples of 99 goats belonging to six different flocks. A panel of polymorphic heterologous microsatellite loci was used to genotype individuals. The microsatellite markers ... more

T.S. Câmara; J.F. Nunes; F.M. Diniz; G.R. Silva; A.M. Araújo
12/19/2016
Conservation of Natural Resources; DNA, Plant; Endangered species; Fabaceae; Genetic markers; Genetic testing; Polymorphism, Genetic; Random Amplified Polymorphic DNA Technique; South America

Apuleia leiocarpa (Vogel) J.F. MacBride is a hardwood species native to South America, which is at serious risk of extinction. Therefore, it is of prime importance to examine the genetic diversity of this species, information required for developing conservation, sustainable management, and breeding strategies. Although scarcely used in recent years, random amplified polymorphic DNA markers ... more

K.H. Lencina; E.R. Konzen; S.M. Tsai; D.A. Bisognin
12/02/2016
DNA, Plant; Evolution, Molecular; Expressed sequence tags; Genetic variation; Genome, Plant; Microsatellite Repeats; Phylogeny; Saccharum

We analyzed 80 plants of the sugarcane (Saccharum spp) variety 'RB867515' in order to investigate its diversity and genetic structure at the molecular level. Four simple sequence repeat (SSR) loci (UGSM51, SMC1237, SEGMS1069, and UGSM38) and five expressed sequence tag (EST)-SSR loci (ESTA68, ESTB92, ESTB145, ESTC66, and ESTC84) were used as molecular markers. The polymorphic loci rate was 66. ... more

G.B. Maranho; R.C. Maranho; R. Desordi; A.F. das Neves; C.A. Mangolin; M.F.P.S. Machado
3/08/2017
Alleles; DNA, Plant; Expressed sequence tags; Genetic variation; Heterozygote; Microsatellite Repeats; Polymorphism, Genetic; Reproduction; Saccharum

The reduction in sugarcane productivity in subsequent cutting stages may be related to a gradual decrease of the allele number and mean observed heterozygosity (H) in the sugarcane ratoon. This hypothesis was tested assessing the number of alleles and H values in 10 expressed sequence tag microsatellites (Est-SSR loci) of the sugarcane varieties RB72454 and RB867515 in different cutting stages ... more

R. Augusto; R.C. Maranho; C.A. Mangolin; J.C.Bespalho Filho; M.F.P.S. Machado
4/05/2017
Chromosomes, Plant; DNA methylation; DNA, Plant; Epigenesis, Genetic; Evolution, Molecular; Genetic variation; Genome, Plant; Microsatellite Repeats; Polyploidy; Random Amplified Polymorphic DNA Technique; Triticum

We used the conventional and methylation-sensitive randomly amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) analyses to assess genome-wide changes and explore the relationships between genetic and epigenetic variations among individuals of a newly synthesized allohexaploid wheat line whose genomic constitution is identical to that of the natural common wheat, compared ... more

T. Qiu; Y.Z. Dong; X.M. Yu; N. Zhao; Y.F. Yang

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