Research Article

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2017 Aug 17
Animals; Diptera; DNA, Mitochondrial; Electron Transport Complex IV; Glycine max; Insect Proteins; Paraguay

We provided the first scientific record of Melanagromyza sojae (Zehntner, 1900), through molecular characterization of partial mtDNA COI gene, that confirms the occurrence of this pest in Paraguay. Previously reported in Brazil, an outbreak of larvae of M. sojae known as the soybean stem fly (SSF) that belongs to the family Agromyzidae, was also noted in soybean fields from the Canindeyú, Alto ... more

J.V.C. Guedes; J.A. Arnemann; L.E. Curioletti; L.M. Burtet; M.L. Ramírez-Paredes; D. Noschang; I. de Oliveira; W.T. Tay
08/17/2017
Animals; Gene Amplification; Gene flow; Gene Transfer, Horizontal; Linkage disequilibrium; Microsatellite Repeats; Perciformes; Polymorphism, Genetic

In this study, 10 polymorphic microsatellite markers were developed in Scomber japonicus and were examined on 30 individuals collected from the North Pacific. The number of alleles per locus ranged from 4 to 17. The observed and expected heterozygosities per locus ranged from 0.2759 to 0.8621 and from 0.43071 to 0.9177, respectively. The polymorphism information content (PIC) was from 0.3931 ... more

F.F. Chen; C.Y. Ma; L.P. Yan; H. Zhang; W. Wang; Y. Zhang; L.B. Ma
08/17/2017
Animals; Enterotoxigenic Escherichia coli; Escherichia coli Infections; Escherichia coli Proteins; Feces; Humans; Molecular Diagnostic Techniques; Multiplex polymerase chain reaction; Sensitivity and Specificity; Water Microbiology

Diarrhea is considered the second most common cause of infant mortality worldwide. The disease can be caused by many different pathogens, including diarrheagenic Escherichia coli (DEC), which includes the pathotypes enterotoxigenic E. coli (ETEC), enteroinvasive E. coli (EIEC), enteroaggregative E. coli (EAEC), Shiga toxin-producing E. coli (STEC), and enteropathogenic E. coli (EPEC). To ... more

J.W. Vendruscolo; T.L. Waldrich; G.I.A. Saikawa; J.S. Pelayo; R.K.T. Kobayashi; G. Nakazato; S.P.D. Rocha
08/31/2017
Animals; DNA Barcoding, Taxonomic; DNA, Ribosomal Spacer; Genome, Insect; Hymenoptera; Lepidoptera; Microsatellite Repeats

In Brazil, the species Diatraea flavipennella and D. saccharalis play an important role in the sugar and alcohol agribusiness by causing many damages in sugarcane fields. The egg, larva, pupa, and adult stages are very morphologically similar between these species, and the identification can be confused. The internal transcribed spacer 2 (ITS 2) from ribosomal DNA has important features as ... more

M.S.Regueira Neto; R.P. Barros; M.A.J. Morais; V.Q. Balbino; V. Loreto
09/21/2017
Animals; Evolution, Molecular; Hybridization, Genetic; Lepidoptera; Polymorphism, Genetic

Helicoverpa armigera is the most significant pest of agriculture in Asia, Europe, Africa, and Australasia, causing damage to crops greater than US$2 billion annually and until 2013 it was not detected in Brazil. Helicoverpa zea is restricted to the American continent and is important to corn and a secondary pest of cotton and tomatoes. The wide range of crops exploited by H. armigera (mainly ... more

H.M. Lopes; C.S. Bastos; L.S. Boiteux; J. Foresti; F.A. Suinaga
09/27/2017
Animals; Blastocyst; Carps; Fish Proteins; Fructose-Bisphosphate Aldolase; Gene Expression Regulation, Developmental; Organ Specificity

Aldolase is a key enzyme involved in glycolysis, gluconeogenesis, and the pentose phosphate pathway. To establish the expression patterns of all three aldolase isozyme genes in different tissues and during early embryogenesis in lower vertebrates, as well as to explore the functional differences between these three isozymes, the grass carp was selected as a model owing to its relatively high ... more

J.J. Fan; J.J. Bai; D.M. Ma; L.Y. Yu; P. Jiang
09/21/2017
Animals; Brazil; Dogs; Feces; Genome, Viral; Parvoviridae Infections; Parvovirus, Canine; Phylogeny; Polymorphism, Genetic; Recombination, Genetic

With the objective of characterizing Canine parvovirus (CPV) from some suspected fecal samples of dogs collected from the Veterinarian Hospital in Belém city, five positive samples were found by PCR assay and an update molecular characterization was provided of the CPV-2 circulation in Belém. Through sequencing of the complete DNA sequences (NS1, NS2, VP1, and VP2 genes), the CPV-2 strain was ... more

S.P. Silva; L.N.P.P. Silva; E.D.L. Rodrigues; J.F. Cardoso; F.N. Tavares; W.M. Souza; C.M.P. Santos; F.M.S. Martins; I.S. Jesus; T.C. Brito; T.P.C. Moura; M.R.T. Nunes; L.M.N. Casseb; S. Filho; A.R. Casseb
09/21/2017
Animals; Cadmium; Cadmium Poisoning; Eels; Fish Proteins; Fresh Water; Liver; Metallothionein; Thailand; Water Pollution, Chemical

Cadmium (Cd) is produced mainly as a by-product of zinc mining. In Thailand, the largest zinc mine is located in the Mae Sot district, Tak Province. Samples of Monopterus albus were collected from paddy fields in 4 sites, three downstream and one upstream from the zinc mine. The upstream site was considered to be uncontaminated while the three downstream sites were considered to be ... more

M. Wahid; S. Prasarnpun; N. Yimtragool
09/27/2017
Animals; Gene Expression Regulation, Developmental; Rabbits; Retina; RNA, Messenger; Rod Opsins; Thy-1 Antigens

Melanopsin is the photopigment of intrinsically photosensitive retinal ganglion cells that mediate non-visual responses to light. The aim of this study was to describe and analyze melanopsin gene expression in the rabbit retina at different ages and compare its expression with the prototypic gene of retinal ganglion cells (Thy-1 gene). Expression levels of OPN4, Thy-1, and GADPH genes were ... more

M.M. Lira-Carrera; B.E. Gutiérrez-Amavizca; L.J. Álvarez-Araujo; M. Aguirre-Ramírez; J.A. Pérez-León
09/21/2017
Animals; Biomarkers, Tumor; Conserved sequence; Helminth Proteins; Protein Binding; Protein domains; Protein stability; Sequence Homology; Tumor Protein, Translationally-Controlled 1; Tylenchoidea

Plant parasitic nematodes infect a wide range of hosts representing the largest source of biotic stress experienced by plants. Meloidogyne genus comprises the most important parasitic nematodes, also known as root-knot nematodes. These parasitic organisms obtain nutrients to support their development through complex interactions with their hosts. The translationally-controlled tumor protein ( ... more

R.M.Moraes Filho; A.F. Menezes; L.S.S. Martins

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