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Alleles; Antineoplastic Combined Chemotherapy Protocols; Carcinoma, Non-Small-Cell Lung; Cisplatin; DNA-Binding Proteins; Genotype; Humans; Lung Neoplasms; Neoplasm Staging; Polymorphism, Genetic; Treatment outcome; X-ray Repair Cross Complementing Protein 1

Non-small cell lung cancer (NSCLC) is the most common cancer globally. The XRCC1 protein interacts with ligase and poly(ADP-ribose) polymerase to repair cisplatin-induced DNA damage. The authors of previous studies have reported XRCC1 Arg399Gln, Arg280His, and Arg194Trp polymorphisms and advanced NSCLC prognosis, but the results are inconclusive. We investigated the association between ... more

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Animals; Antimutagenic Agents; Cell line; Cell proliferation; DNA damage; Dose-Response Relationship, Drug; Plant Extracts; Rats; Vanillic Acid

The consumption of healthy and natural foods has increased over the last few years, primarily because these foods are rich in substances with biological properties of interest, such as exerting anticancer effects and decreasing oxidative stress in living tissues. These foods support adequate nutrition, maintain health, and improve quality of life. Vanillic acid (VA) is a phenolic compound used ... more

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Adipose tissue; Animals; Cell differentiation; Cell proliferation; Estradiol; Female; Pregnancy; Progesterone; Rats; Stem cells

Stem cell therapy faces many problems including poor survival rates and low viability. Enhancing the biological functions of stem cells improves efficacy of therapies. Estrogen, whose levels are elevated during pregnancy, affects the properties of bone marrow mesenchymal stem cells. Evidence suggests that adipose-derived stem cells (ADSCs), which are a type of adult mesenchymal stem cells, can ... more

J.N. Li; Y. Zhang; Y.F. Wang; J.Y. Chen
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Animals; Biomarkers; Drugs, Chinese Herbal; Endometrium; Estrous cycle; Female; Flavonoids; Immunohistochemistry; Rats

This study aimed to investigate the effect of icariin (ICA) on thin endometrium in a rat model. To this end, 6- to 8-week-old female Sprague Dawley rats (105) were randomly divided into 7 groups: untreated, vehicle-treated (lavage with NaCl), high-dose ICA (lavage with ICA at 200 mg∙kg∙day), medium-dose ICA (lavage ICA at 100 mg∙kg∙day), low-dose ICA (lavage with ICA at 50 mg∙kg∙day), sham ... more

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Animals; Carbohydrates; Cell Line, Tumor; Cell proliferation; DNA damage; Food Handling; Food Quality; Food storage; Fruit and Vegetable Juices; Rats; Ultraviolet Rays; Vitamins; Vitis

Integral grape juice is extracted from the grape through processes that allow the retention of their natural composition. However, due to the severity of some processes, fruit juices can undergo changes in their quality. The present study evaluated the cytotoxic and mutagenic effects of integral grape juice by a cytokinesis-blocked micronucleus assay in Rattus norvegicus hepatoma cells (HTC) ... more

E. Düsman; I.V. Almeida; E.P. Pinto; L. Lucchetta; V.E.P. Vicentini
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Animals; Cell Line, Tumor; Cell Movement; Cell proliferation; Endoglin; Female; Gene Expression Regulation, Neoplastic; Humans; Mice; Mice, Nude; MicroRNAs; Neoplasm Invasiveness; Neoplasm Transplantation; Neovascularization, Pathologic; Thyroid Neoplasms

Thyroid cancer is the most common type of endocrine tumor. MicroRNAs (miRNAs) play a critical role in a variety of diseases, especially cancer occurrence and progression. However, the specific mechanism by which miRNAs trigger disease states has not been fully elucidated. This study aims to investigate the role of miR-27a in thyroid cancer cells. A wound healing assay was adopted to examine ... more

Y.L. Wang; W.G. Gong; Q.L. Yuan
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Animals; Biomarkers; Cell line; G-Protein-Coupled Receptor Kinase 2; Humans; Mechanical Phenomena; Mice; Osteoblasts; Phosphorylation; Receptors, Purinergic P2Y2; Signal transduction

Bone desensitization after mechanical loading is essential for bone to adapt to its mechanical environment. However, the desensitization mechanism is unknown. Previous studies suggest that G protein-coupled receptors (GPCRs), including P2Y and parathyroid hormone receptors, play important roles in osteoblast mechanobiology. Thus, for the present research, we examined the role of G protein- ... more

Y. Xing; Y. Gu; X. Shan; L. Wang; J. You
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Algorithms; Amino acid sequence; Amino acids; Animals; binding sites; Computational biology; Databases, Protein; Humans; Hydrophobic and Hydrophilic Interactions; Models, Theoretical; Neural Networks, Computer; Phosphorylation; Proteins; Reproducibility of Results; Support vector machine

Phosphorylation is an important part of post-translational modifications of proteins, and is essential for many biological activities. Phosphorylation and dephosphorylation can regulate signal transduction, gene expression, and cell cycle regulation in many cellular processes. Phosphorylation is extremely important for both basic research and drug discovery to rapidly and correctly identify ... more

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Animals; Bone morphogenetic protein 2; Genetic Therapy; Humans; Mandible; Osteogenesis, Distraction; Rabbits; Transforming Growth Factor beta1; Vascular Endothelial Growth Factor A

Transforming growth factor-β1 (TGF-β1) is a member of the TGF-β superfamily, and plays an important role in promoting various stages of intramembranous and endochondral bone formation. It is one of the major growth factors that influence new bone formation in the distraction gap during distraction osteogenesis (DO). The major problem of DO is the time required for the treatment. Reports show ... more

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Animals; Gene frequency; Heterozygote; Humans; Polymorphism, Genetic; Software

The gene diversity or expected heterozygosity (H) is based on the allele frequency and is often used as a measure of genetic variability of populations. Knowing the pattern of spatial distribution of H can be useful for determining strategies of conservation and sampling of collections of individuals. In addition, it can allow one to detect genetic boundaries in a landscape. We adapted a ... more

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