Research Article

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2017 Mar 30
Alleles; Amelogenin; Asians; China; Chromosomes, Human, X; Chromosomes, Human, Y; DNA fingerprinting; Ethnicity; Female; Forensic genetics; Gene frequency; Genetics, Population; Haplotypes; Humans; male; Microsatellite Repeats; Paternity; Polymorphism, Genetic

The sex-linked short tandem repeats (STR), Y-STR and X-STR, are important for autosomal STRs in forensic paternity testing. We evaluated the forensic parameters of 19 Y-STRs and 16 X-STRs in the Han population of Shandong province, China. A Goldeneye 20Y kit (DYS391, DYS389I, DYS390, DYS389II, DYS348, DYS456, Y-GATA-H4, DYS447, DYS19, DYS392, DYS393, DYS388, DYS439, DYS635, DYS448, DYS460, ... more

H.M. Gao; C. Wang; S.Y. Han; S.H. Sun; D.J. Xiao; Y.S. Wang; C.T. Li; M.X. Zhang
4/5/2017
Alleles; DNA; DNA Primers; Forensic genetics; Gene frequency; Genetics, Population; Humans; Microsatellite Repeats; Molecular Diagnostic Techniques; Multiplex polymerase chain reaction; Polymorphism, Genetic

This study aimed to develop a short tandem repeat (STR) multiplex system, made up of 22 highly informative STR loci, for application in forensic genetics. The system comprised 21 polymorphic autosomal loci (D3S1358, TH01, D21S11, D18S51, Penta E, D5S818, D13S317, D7S820, D16S539, CSF1PO, Penta D, vWA, D8S1179, TPOX, FGA, D2S441, D17S1301, D19S433, D18S853, D20S482, and D14S1434) and the ... more

R.G. Rodovalho; E.L. Rodrigues; G.S. Santos; L.M. Cavalcanti; P.R. Lima; A.G. Rodovalho; R.G. Vital; M.A.D. Gigonzac; A.D. da Cruz
5/31/2017
Bromeliaceae; Gene Amplification; Gene frequency; Heterozygote; Inbreeding; Microsatellite Repeats; Polymorphism, Genetic

The Alcantarea patriae is a Bromeliaceae endemic to the inselbergs of the Atlantic Forest. This taxon, described in the year of 2007 by Versieux & Wanderley, presents restricted and fragmented distribution outside conservation units. Studies to evaluate the genetic structure of its populations can contribute to the conservation and management strategies for the species. In this study, 31 ... more

A.G. Pereira; U.C.S. Bernardi; V.C. Manhães; R.S. Ferreira; F.D. Miranda
8/17/2017
Eucalyptus; Gene frequency; Genotype; Linkage disequilibrium; Microsatellite Repeats; Polymorphism, Genetic

Eucalyptus urophylla is an important species in the Brazilian forest sector due to its rapid growth rates and resistance to disease. The aim of this study was to verify Mendelian inheritance, genetic linkage, and genotypic disequilibrium for 15 microsatellite loci, with the goal of producing a robust set of genetic markers. Mendelian inheritance and genetic linkage analyses were carried out ... more

S. Pupin; L.N. Rosse; I.C.G. Souza; J. Cambuim; C.L. Marino; M.L.T. Moraes; A.M. Sebbenn
8/31/2017
Arecaceae; Evolution, Molecular; Gene flow; Gene frequency; Heterozygote; Linkage disequilibrium; Microsatellite Repeats; Polymorphism, Genetic

We assessed the level and distribution of genetic diversity in three species of the economically important palm genus Astrocaryum located in Pará State, in northern Brazil. Samples were collected in three municipalities for Astrocaryum aculeatum: Belterra, Santarém, and Terra Santa; and in two municipalities for both A. murumuru: Belém and Santo Antônio do Tauá and A. paramaca: Belém and ... more

N.P. Oliveira; M.S.P. Oliveira; L.C. Davide; S. Kalisz
9/27/2017
Arecaceae; environment; Gene frequency; Genotype; Microsatellite Repeats; Phenotype; Polymorphism, Genetic; Selection, Genetic

This study uses ISSR molecular markers to characterize the demographic pattern, and spatial genetic structure (SGS) at different life stages of development (cohorts) in a natural population of Copernicia prunifera in the Rio Grande do Norte State, Brazil. All individuals were sampled and georeferenced in a 0.55-ha plot. The demographic analyses showed a clustered pattern in the first-distance ... more

L.G. Pinheiro; K.P.T. Chagas; A.S.M. Freire; M.C. Ferreira; C.G. Fajardo; F.A. Vieira
9/27/2017
Ecuador; Gene frequency; Humans; Microsatellite Repeats; Polymorphism, Genetic; Population

One hundred and eighty-two samples of unrelated people who requested the paternity test at the Molecular Biology and Genetics Laboratory of the Catholic University of Cuenca-Ecuador in the province of Azuay were studied, except for the D1S1656 (180 samples) and SE33 (89 samples) markers. The STRs D22S1045, D3S1358, VWA, D16S539, D2S1338, D8S1179, D21S11, D18S51, D19S433, TH01, FGA, D1S1656, ... more

P.P. Orellana; C.F. Andrade; C.L. Arciniegas; G.C. Iannacone
9/27/2017
Animals; Anovulation; Brain; Female; Interleukin-6; Leptin; Lipid metabolism; Mice; Mice, Obese; Receptor Protein-Tyrosine Kinases; Receptors, GABA; Receptors, Muscarinic; Receptors, Serotonin; Synaptic Transmission

Polycystic ovary syndrome is the classic example of loss of functional cyclicity and anomalous feedback. In this case, the excessive extra-glandular production and conversion of androgens to estrogens are the pathophysiological basis of the chronic anovulation. The literature describes an experimental model of the polymicrocystic ovary in obese diabetic mice with insulin resistance. The fact ... more

M.A. Azevedo; I.D.C.G. Silva
2/8/2017
Brazil; Breeding; Crosses, Genetic; Fusarium; Genetic variation; Genotype; Multivariate analysis; Plant Diseases; Tracheophyta

The multivariate analyses are useful tools to estimate the genetic variability between accessions. In the breeding programs, the Ward-Modified Location Model (MLM) multivariate method has been a powerful strategy to quantify variability using quantitative and qualitative variables simultaneously. The present study was proposed in view of the dearth of information about popcorn breeding ... more

R.N.F. Kurosawa; A.T. do Amara Junior; F.H.L. Silva; D. Santos; M. Vivas; S.H. Kamphorst; G.F. Pena
2/8/2017
Brazil; Breeding; Crosses, Genetic; Genetics, Population; Genotype; Phenotype; Zea mays

In Brazil, the total production of maize has increased continuously due to the advances in technology but also to the new frontiers for the maize crop, including the expansion of the second crop (safrinha; usually maize after soybean). Therefore, the exploitation of new sources of germplasm seems to be imperative to attend the actual and future demands for modern cultivars adapted to the ... more

C.M. Silva; J.B.Miranda Filho; U.C. Mendes; E.F. Reis

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