Research Article

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12/19/2016
Animals; Feces; Foxes; Genetic markers; Genotyping Techniques; Heterozygote; Microsatellite Repeats

The Ezo red fox (Vulpes vulpes schrencki), a subspecies endemic to Hokkaido island, Japan, is a known host species for the tapeworm Echinococcus multilocularis. To develop tools for molecular ecological studies, we isolated 28 microsatellite regions from the genome of Ezo red fox, and developed 18 polymorphic microsatellite markers. These markers were characterized using 7 individuals and 22 ... more

T. Tada; Y. Seki; Y. Kameyama; Y. Kikkawa; K. Wada
12/23/2016
Actins; Animals; Fishes; Genetic markers; Real-time polymerase chain reaction; Reference Standards; RNA, Ribosomal, 18S

Tambaqui, Colossoma macropomum, is the main native freshwater fish in Brazilian aquaculture. Therefore, intensive research pressure has been applied to the species to support new technologies for tambaqui farming. Molecular biology represents a tool that can be used to investigate every field of applied biology, from fish physiology to the effects of climate change. Based on the importance of ... more

A.R. Nascimento; G.F. Silva; G.F. Gualberto; F.L. Almeida
2/16/2017
Animals; Gene expression profiling; Gene Expression Regulation; Genetic markers; Oligonucleotide Array Sequence Analysis; Phenotype; Quantitative trait loci; Sheep; Wool

Fiber diameter is a useful indicator of wool traits and it is the main determinant of wool quality and value. A comparative study was conducted to analyze the abundance and expression of 13 candidate genes using expression profile microarray analysis and to identify novel molecular markers associated with wool traits to provide a molecular basis for improving wool quality in sheep. Genes ... more

Y.Z. Tian; T. Usman; K.C. Tian; J. Di; X.X. Huang; X.M. Xu; H. Tulafu; W.W. Wu; X.F. Fu; Y. Bai; M. Tuerxun; A. Maimaiti
2/16/2017
Cell Nucleus; Chloroplasts; DNA Primers; Genetic markers; Microsatellite Repeats; Mimosa; Sequence Analysis, DNA; Species Specificity; Tetraploidy

Mimosa scabrella Benth., popularly known as ''bracatinga'', is a pioneer and endemic species of Brazil, occurring in Mixed Ombrophilous Forest associated with Brazilian Atlantic Rainforest biomes. It is a fast-growing tree of the Fabaceae family that facilitates the dynamics of ecological succession. SSR development, when there is no genome sequence, is time and labor intensive and there are ... more

F.A. Saiki; A.P. Bernardi; M.S. Reis; H. Faoro; E.M. Souza; F.O. Pedrosa; A. Mantovani; A.F. Guidolin
3/16/2017
Animals; Base Sequence; Conserved sequence; Disease resistance; Evolution, Molecular; Genetic markers; Genetic variation; Genotype; Microsatellite Repeats; Phylogeny; Plant Diseases; Plant Proteins; Psidium; Tylenchoidea

Guava (Psidium guajava L.) crop is severely affected by the nematode Meloidogyne enterolobii. Native Psidium species have been reported as sources of resistance against this nematode. Knowledge on the molecular relationship between Psidium species based on plant resistance gene analogs (RGA) can be useful in the genetic breeding of guava for resistance to M. enterolobii. In this study, RGA ... more

L.R. Noia; A.C. Tuler; A. Ferreira; M.F.S. Ferreira
4/20/2017
Animals; Cetacea; DNA Barcoding, Taxonomic; Electron Transport Complex IV; Endangered species; Genetic markers; Reference Standards; Sequence alignment

Molecular identification is very useful in cases where morphology-based species identification is not possible. Examples for its application in cetaceans include the identification of carcasses of stranded animals in advanced state of decomposition and body parts that are illegally traded. One DNA region that is often used for molecular identification is the Folmer region of the mitochondrial ... more

L.H.O. Falcão; A.S. Campos; J.E.P. Freitas; M.A.A. Furtado-Neto; V.V. Faria
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Alleles; Animals; Expressed sequence tags; Genetic markers; Heterozygote; Microsatellite Repeats; Perciformes; Polymorphism, Genetic; Transcriptome

Expressed sequence tags (ETSs) are the sources of microsatellite development. In this study, we isolated and characterized microsatellite markers for Odontobutis potamophila by using Illumina RNA-sequencing. We sequenced a large number of ESTs and screened 200 potential microsatellites. Consequently, a total of 56 novel polymorphic microsatellite repeat markers were identified in thirty-two ... more

P.P. Wang; M. Ren; S.Q. Chen; S.W. Yin; C. Zhao; H.Y. Zhang; X.R. Li; Q.Q. Cao; G.Q. Zhou
7/6/2017
Adult; Animals; Case-control studies; Dermatophagoides pteronyssinus; Female; Humans; Interleukin-18; male; Middle Aged; Polymorphism, Single Nucleotide; Promoter Regions, Genetic; Rhinitis, Allergic, Perennial; Thailand

Allergic rhinitis (AR) is an IgE-mediated inflammation of the nasal membranes, which is naturally triggered by aeroallergens. House dust mites (HDM) are the most common inhalant allergens. Interleukin-18 (IL-18) has been established as an essential cytokine that can activate the generation of IgE. This randomized controlled study aimed to identify the possible relationship of the genetic ... more

A. Tungtrongchitr; J. Jumpasri; N. Sookrung; N. Visitsunthorn; P. Tantilipikorn; O. Piboonpocanan; N. Indrawattana; R. Tungtrongchitr; W. Chaicumpa
2016 Dec 19
Amniotic fluid; Chromosomes, Human; Chromosomes, Human, Pair 13; Chromosomes, Human, Pair 18; Chromosomes, Human, Pair 21; Chromosomes, Human, X; Chromosomes, Human, Y; Female; Gene frequency; Genetic variation; Humans; Iran; male; Microsatellite Repeats; Pregnancy; Prenatal diagnosis; Whites

Quantitative fluorescent polymerase chain reaction (QF-PCR), in recent years, has been accepted as a rapid, high throughput, and sensitive method for prenatal diagnosis of common chromosomal aneuploidies. Since short tandem repeats (STRs) are the cornerstone of QF-PCR technique, selection of the most polymorphic STR markers is an essential step for a successful QF-PCR assay. The genetic ... more

J. Saberzadeh; M.R. Miri; M.B. Tabei; M. Dianatpour; M. Fardaei
2017 Mar 15
Adolescent; Adult; Aged; Aged, 80 and over; Asians; Child; Child, Preschool; China; Duffy Blood-Group System; Female; Gene frequency; Genotype; Humans; Infant; Kell Blood-Group System; male; Middle Aged; MNSs Blood-Group System; Young Adult

Human blood groups are a significant resource for patients, leading to a fierce international competition in the screening of rare blood groups. Some rare blood group screening programs have been implemented in western countries and Japan, but not particularly in China. Recently, the genetic background of ABO and Rh blood groups for different ethnic groups or regions in China has been focused ... more

G.Y. Lin; X.L. Du; J.J. Shan; Y.N. Zhang; Y.Q. Zhang; Q.H. Wang

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