Research Article

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3/15/2017
Animals; Breeding; Cells, Cultured; Female; Gene expression profiling; Gene Expression Regulation; Goats; Oocytes; Oogenesis; Single-Cell Analysis; Transcriptome

Juvenile in vitro embryo transfer is an important animal reproductive technology that can shorten the generation interval of livestock, explore the reproductive potential of dams with excellent genetic traits, accelerate genetic progress and production efficiency of the herd, and provide a wealth of genetic resources for livestock breeding. However, oocytes from kids do not develop as well as ... more

X.Y. Yin; G.H. Cheng; H.Y. Guo; Q. Wang; Y.J. Li; H. Zhang
03/16/2017
Animals; Aquaculture; Cichlids; Fish Proteins; Gene expression; Gene Expression Regulation, Developmental; Muscle, Skeletal; Myostatin; Ponds

Tissue growth in most fishes occurs by muscular hyperplasia and hypertrophy, which are influenced by different regulatory factors, such as myostatin. The current study evaluated the influence of cultivation in hapas and earthen ponds on the diameter of white muscle fibers and on the myostatin (MSTN-1) gene in GIFT and Supreme varieties of tilapia. Fish of both varieties were reared for 204 ... more

E.C.S. Lima; J.A. Povh; R.A.A. Otonel; J.H. Leonhardt; A.A. Alfieri; S.A. Headley; F.P. Souza; A.R. Poveda-Parra; P.J. Furlan-Murari; N.M. Lopera-Barrero
3/16/2017
Animals; Aquaculture; Cichlids; Fish Proteins; Gene expression; Gene Expression Regulation, Developmental; Muscle, Skeletal; Myostatin; Ponds

Tissue growth in most fishes occurs by muscular hyperplasia and hypertrophy, which are influenced by different regulatory factors, such as myostatin. The current study evaluated the influence of cultivation in hapas and earthen ponds on the diameter of white muscle fibers and on the myostatin (MSTN-1) gene in GIFT and Supreme varieties of tilapia. Fish of both varieties were reared for 204 ... more

E.C.S. Lima; J.A. Povh; R.A.A. Otonel; J.H. Leonhardt; A.A. Alfieri; S.A. Headley; F.P. Souza; A.R. Poveda-Parra; P.J. Furlan-Murari; N.M. Lopera-Barrero
2/16/2017
14-3-3 Proteins; Adaptation, Physiological; Animals; Fish Proteins; Fishes; Gene expression; Gene Expression Regulation; Gills; Salinity

Fishes adapt to salinity changes primarily through osmotic pressure regulation, a process often associated with several genes, including 14-3-3a, NKCCla, A-14, and Na-K-ATPaseβ. The present study investigated the differential expression of genes 14-3-3a, NKCCla, A-14, and Na-K-ATPaseβ in the gill tissue of Mugil cephalus acclimated to low salinity. Susceptibility relationships between the four ... more

L. Li; M. Jiang; X.Q. Shen
3/30/2017
Animals; Cattle; Female; Gene expression; In Vitro Techniques; Mammary Glands, Animal; Mastitis, Bovine; Milk; Models, Animal; Perfusion; Real-time polymerase chain reaction; Ubiquitin

Intramammary infections are one of the main causes of productivity loss in dairy cows. To better understand the immune system response and to avoid the use of live animals, we validated the use of isolated bovine udder as an ex situ model. Six mammary glands were collected from cows ready for culling. Three udders were perfused with Tyrode's solution and three were not-perfused. During six ... more

I.S.B. Pinto; I. Fonseca; H.M. Brandão; J.C. Gern; A.S. Guimarães; W.A. Carvalho; M.A.V.P. Brito; L.F. Viccini; M.F. Martins
12/02/2016
Adult; Alanine; Arginine; Asian People; Diabetes Mellitus, Type 2; Female; Genetic predisposition to disease; Genotype; Glutamine; Humans; Leucine; male; Middle Aged; Polymorphism, Single Nucleotide; Regression Analysis; Superoxide dismutase; Threonine

The most common type of endocrine disease is type 2 diabetes mellitus (T2DM); genetic factors contribute to the development to T2DM. In this study, we investigated the role of the Leu53Leu, Arg213Gly, and Ala40Thr polymorphisms in extracellular superoxide dismutase (EC-SOD) gene in the development of T2DM in a Chinese population. DNA was extracted from peripheral blood samples obtained from ... more

Y.M. Yang; X.R. Xie; A.L. Jin
12/02/2016
Adult; Aged; Aged, 80 and over; Asian People; China; Chromosome aberrations; Chromosomes, Human; Female; Humans; Karyotyping; Lymphoma, Non-Hodgkin; male; Middle Aged; Young Adult

The incidence of non-Hodgkin lymphoma (NHL) in China is increasing and is attracting attention as a topic of research. The percentage of NHL cases in ethnic Uighur people is also gradually increasing. We therefore recruited Uighur people with NHL to investigate the correlation between genetic alternations and clinical/pathological features in an attempt to determine their clinical significance ... more

L. Xu; X.G. Zou; X. Wang; A.B.L.M.T. Hairesa; J.J. Liu
12/02/2016
Actinin; Adult; Ankle Injuries; Asian People; China; Female; Gene frequency; Genetic association studies; Genetic predisposition to disease; Humans; male; Polymorphism, Single Nucleotide; Young Adult

In this study, we investigated the association between ACTN3 R577X polymorphism and non-acute ankle sprain by measuring the allele frequency and genotype distribution of ACTN3 in a Chinese Han population. We recruited 100 patients with non-acute ankle sprain and 100 healthy controls with no history of ankle injuries. Mass spectrometric analysis of single nucleotide polymorphism was used to ... more

B. Qi; J.Q. Liu; G.L. Liu
12/02/2016
Administration, Oral; Animals; Bone marrow cells; Cell proliferation; Cells, Cultured; Guatemala; Humans; Lymphocytes; male; Mexico; Micronucleus tests; Mutagenicity Tests; Plant Extracts; Rats; Rubus; Sister chromatid exchange; Toxicity Tests, Subchronic

Rubus coriifolius Focke is a wild plant from the Rosaceae family. It grows in both Guatemala and Mexico. The polar extract of the aerial parts of this plant has antibacterial, anti-inflammatory, and anti-protozoal activities. These properties may explain the traditional use of this plant. In vivo and in vitro assays were used to assess the genotoxic and toxic effects of an ethanol extract of ... more

S. González-Hernández; D. González-Ramírez; M.I. Dávila-Rodríguez; A. Jimenez-Arellanez; M. Meckes-Fischer; S. Said-Fernández; E.I. Cortés-Gutiérrez
12/02/2016
Female; Humans; Macrophage Activation; Macrophages; male; Prognosis; Proportional Hazards Models; Stomach neoplasms; Survival rate

Tumor-associated macrophages (TAMs), which play a crucial role in the tumor microenvironment, can be divided into M1 and M2 phenotypes, these phenotypes may exert opposite effects on the prognoses of patients with gastric cancer (GC). The association between TAMs and GC is contentious. Thus, a meta-analysis of 12 studies (incorporating 1388 patients) retrieved from the Cochrane Library, PubMed ... more

X.L. Wang; J.T. Jiang; C.P. Wu

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