Research Article

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Comparative transcriptome; De novo assembly; Illumina sequencing; Lymph organ; Procambarus clarkii; White spot syndrome virus

The red swamp crayfish (Procambarus clarkii) is one of the most economically important farmed aquatic species in China. Compared with its relatively well-known antibacterial and antifungal mechanisms, the antiviral mechanism is still unclear. We used Illumina-based RNA sequencing and bioinformatic technology to obtain high-quality sequence reads from the ... more

Z.Q. Du; Z.Q. Du
FIASCO; Genetic markers; Lateolabrax japonicus; Microsatellites

Lateolabrax japonicus, an economically important species, is widely consumed in the offshore coasts of China, Korea, and Japan. We identified 10 new L. japonicus microsatellite markers, using a modified protocol of fast isolation by AFLP of sequences containing repeats. Thirty L. japonicus individuals were collected from Xiamen, China, to ... more

B.B. Li; Z.B. Li; J.B. Shangguan; Y.F. Ning; Y. Yuan; Y.S. Huang; X.Q. Mao
Censored data; GBLUP; Mixed model; Survival models

Age at the time of slaughter is a commonly used trait in animal breeding programs. Since studying this trait involves incomplete observations (censoring), analysis can be performed using survival models or modified linear models, for example, by sampling censored data from truncated normal distributions. For genomic selection, the greatest genetic gains can be ... more

V.S. Santos; M. Filho; M.D.V. Resende; C.F. Azevedo; P.S. Lopes; S.E.F. Guimarães; F.F. Silva
Epinephelus coioides; IGF1Ra; IGF1Rb; Nucleotide diversity; Polymorphism

Orange-spotted grouper (Epinephelus coioides) is one of the most important marine fish and has a high market value. The insulin-like growth factor type 1 receptor (IGF1R) is a component of the insulin-like growth factor signaling system, and demonstrates important roles during growth. Based on information from livestock, we ... more

L. Guo; S. Yang; M.M. Li; Z.N. Meng; H.R. Lin; L. Guo; S. Yang; M.M. Li; Z.N. Meng; H.R. Lin
Calf testis; Germline stem cells; Glial cell line-derived neurotrophic factor; Leukemia-inhibitory factor; Proliferation; Sperm differentiation

Germline stem cells are the only such capable of transmitting genetic information in vivo. The isolation and culture of these cells in vitro provide a unique model to understand sperm differentiation and hence, spermatogenesis and male fertility. In this study, we isolated, purified, and cultured germline stem cells from the testes of newborn ... more

P. Zheng; H. Huang; Y.G. Tian; Y. Zeng; E.O. Adegoke; X. Wang; G.X. Zhang; P. Zheng; H. Huang; Y.G. Tian; Y. Zeng; E.O. Adegoke; X. Wang; G.X. Zhang
Dry matter intake; Feed intake; Performance traits; Residual feed intake; Variance partitioning

Feed intake, feed efficiency, and weight gain are important economic traits of beef cattle in feedlots. In the present study, we investigated the physiological processes underlying such traits from the point of view of systems genetics. Firstly, using data from 1334 Nellore (Bos indicus) cattle and 943,577 single nucleotide polymorphisms (SNPs), a genome- ... more

M.H.A. Santana; M.C. Freua; D.N. Do; R.V. Ventura; H.N. Kadarmideen; J.B.S. Ferraz
Astyanax altiparanae; Geophagus brasiliensis; Microsatellite; RAPD; South America

Streams are very important environments for Neotropical freshwater fish fauna, and possess a high number of species. These small drainages are also highlighted by their intrinsic biological and physicochemical features; however, knowledge on the genetic distribution of fish in these drainages is limited. Therefore, in the present study ... more

D.G. Ferreira; S.C. Lima; W. Frantine-Silva; J.F. Silva; C. Apolinário-Silva; S.H. Sofia; S. Carvalho; B.A. Galindo; D.G. Ferreira; S.C. Lima; W. Frantine-Silva; J.F. Silva; C. Apolinário-Silva; S.H. Sofia; S. Carvalho; B.A. Galindo
Atg6; Beclin 1; Buffy; Parkinson’s disease; Pi3K59F; α-synuclein

Autophagy is a cellular mechanism implicated in the pathology of Parkinson’s disease. The proteins Atg6 (Beclin 1) and Pi3K59F are involved in autophagosome formation, a key step in the initiation of autophagy. We first used the GMR-Gal4 driver to determine the effect of reducing the expression of the genes encoding these proteins on the developing ... more

P.G. M’Angale; B.E. Staveley; P.G. M’Angale; B.E. Staveley
GHR; Growth curve; Sex-linked dwarf chickens

Sex-linked dwarf (SLD) chickens have been widely used in cross breeding of broilers and laying hens. To study the molecular mechanisms underlying growth hormone receptor (GHR) in SLD chickens, the expression profiles of GHR were measured in three growth related tissues (liver, breast, and thigh) in male and female S2 SLD chickens at ... more

H.Y. Huang; Z.H. Zhao; S.F. Li; Z. Liang; C.M. Li; Q.B. Wang
Dipterians; Forensic entomology; Forensic genetics; Short tandem repeats; Uniparental markers

The use of insects to answer questions in criminal investigations, as well as a combination of forensic genetic techniques to obtain human DNA from the organisms, especially necrophagous dipterians, have gained ground in recent decades among researchers and professionals in this area. The objective of our study was to evaluate and ... more

T.C. Oliveira; A.B.R. Santos; K.C.N. Rabelo; C.A. Souza; S.M. Santos; S. Crovella