Research Article

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The arboreal species Spondias mombin L. (Anacardiaceae) is widely distributed in Brazil, where the fruits, known by the common name of cajá, are an important commercial commodity. We evaluated genetic variability among 32 cajá accessions of the Germplasm Collection of Embrapa Meio-Norte using RAPD technique. Reaction conditions for efficient RAPD amplifications were optimized in ... more

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Leporinus friderici, native to the Amazon Basin and popularly known as “piau-três-pintas”, has great ecological and economic importance; it is widely fished and consumed throughout much of tropical South America. Knowledge of the genetic diversity of this native species is important to support management and conservation programs. We evaluated microsatellite loci amplification, ... more

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Genetic variability; Pennisetum purpureum Schum.; RAPD and ISSR markers

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AFLPs; conservation genetics; Genetic variability; Tropical forest fragment

Studies on intraspecific variation can contribute to the development of conservation strategies by identifying units of conservation for threatened species. Aspidosperma polyneuron is a tropical tree of seasonal semideciduous forests that is currently endangered and protected because it has been heavily logged for lumber, although it was once common in Brazil and neighboring ... more

J.O. Damasceno; E.A. Ruas; L.A. Rodrigues; C.F. Ruas; E. Bianchini; J.A. Pimenta; P.M. Ruas
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Gene expression; Genetic variability; Isoenzymes; Ontogenetic stages

Sib-seedlings of 95 strains of the strictly autogamous grass Hordeum euclaston were analyzed by horizontal polyacrylamide gel electrophoresis for four isoenzyme systems at a specific ontogenetic stage. We found differences in the activity of some genes among individuals of this species. Hence, an ontogenetic analysis was carried out to investigate 12 strains at five ontogenetic ... more

J.E. Georg-Kraemer; C.A.S. Ferreira; S.S. Cavalli
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Backcross; Genetic variability; Inbreeding; Microsatellite markers; Parental genome

The limited number of papaya varieties available reflects the narrow genetic base of this species. The use of backcrossing as a breeding strategy can promote increases in variability, besides allowing targeted improvements. Procedures that combine the use of molecular markers and backcrossing permit a reduction of the time required for introgression of genes of interest and appropriate ... more

H.C.C. Ramos; M.G. Pereira; F.F. Silva; L.S.A. Gonçalves; F.O. Pinto; G.Ade Souza Filho; T.S.N. Pereira
11/16/2010
AFLP; Amazon region; Anopheles darlingi; Genetic variability; Malaria vector; Population differences

Anopheles darlingi is the most important malaria vector in Central and South America. After a dramatic reduction of malaria cases in the whole Brazilian territory, with the lowest abundance being attained by 1970, the disease resurged in the Amazon region, where it is now a great public health concern. Consequently, better knowledge about vector species became urgent. We ... more

L.M. Santos; R.A. Gama; A.E. Eiras; C.G. Fonseca
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Biodiversity conservation; Coefficient of similarity; genetic breeding; Genetic variability; Grouping analyses; Molecular marker

The genetic diversity and characteristics of commercial interest of Passiflora species make it useful to characterize wild germplasm, because of their potential use for fruit, ornamental and medicinal purposes. We evaluated genetic diversity, using RAPD markers, of 32 genotypes of Passiflora cincinnata collected from the wild in the region of Vitória da Conquista, Bahia ... more

C.B.M. Cerqueira-Silva; L.D.H.C.S. Conceição; E.S.L. Santos; C.B. Cardoso-Silva; A.S. Pereira; A.C. Oliveira; R.X. Corrêa
10/26/2010
Coefficient of similarity; Conservation; genetic breeding; Genetic variability; Grouping analyses; Molecular marker

In spite of the importance of and the considerable variability observed in Passiflora (Passifloraceae), little is known about the genetic diversity of most of the species of this genus. We evaluated the genetic diversity by RAPD markers in 18 genotypes of Passiflora trintae. The 15 primers generated 112 markers, 84% of which were polymorphic. The genetic distance ... more

C.B.M. Cerqueira-Silva; C.B. Cardoso-Silva; E.S.L. Santos; L.D.H.C.S. Conceição; A.S. Pereira; A.C. Oliveira; R.X. Corrêa
09/08/2010
Anthracnose; common bean; Genetic variability; RAPD markers

Glomerella cingulata f. sp phaseoli is the sexual phase of the fungus Colletotrichum lindemuthianum, the causal agent of common bean anthracnose. This fungus is of great concern, because it causes large economic losses in common bean crops. RAPD markers of five populations of G. cingulata f. sp phaseoli from two Brazilian states were analyzed ... more

E.A. Souza; O.A. Camargo; J.M.A. Pinto

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