Research Article

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05/12/2016
Apoptosis; Brain damage; Hypoxia; miR-152; PTEN

Brain damage caused by perinatal asphyxia is dangerous for neonatal infants, but the mechanism by which it occurs remains elusive. In this study, microRNA-152 (miR-152) expression was induced by low oxygen levels in rat models of hypoxia brain damage, as well as in human brain microvascular endothelial cells (HBMECs) cultured in vitro. Analysis of the sequence of miR-152 revealed ... more

Y.H. Cao; D.G. Li; B. Xu; M.Q. Wang; N. Zhen; L.X. Man; Y.Y. Zhang; M. Chi; Y.H. Cao; D.G. Li; B. Xu; M.Q. Wang; N. Zhen; L.X. Man; Y.Y. Zhang; M. Chi
06/03/2016
CXCR4; EGFR2; Endometriosis; MicroRNA; PTEN; VEGFA

We examined the aberrant microRNA (miRNA) expression profile responsible for the changes in angiogenesis observed in endometriotic lesions. This study revealed characteristic miRNA expression profiles associated with endometriosis in endometrial tissue and endometriotic lesions from the same patient, and their correlation with the most important angiogenic and fibrinolytic factors. miRNA ... more

R.Q. Yang; H. Teng; X.H. Xu; S.Y. Liu; Y.H. Wang; F.J. Guo; X.J. Liu; R.Q. Yang; H. Teng; X.H. Xu; S.Y. Liu; Y.H. Wang; F.J. Guo; X.J. Liu
07/29/2016
Acquired drug-resistance; Pancreatic cancer; Pemetrexed; PI3K/Akt signal transduction pathway; PIK3CA; PTEN

The aim of this study was to investigate the expression of PTEN and PIK3CA in the pemetrexed-resistant human pancreatic cancer cell line Patu8988, and to evaluate their effects on the biological behavior of pancreatic cancer cells. PTEN and PIK3CA gene and protein expressions were detected by reverse transcriptase polymerase chain reaction (RT-PCR) and western blot, respectively, in a ... more

X. Shi; H.T. Gu; S.B. Lin; Y. Zhang; J. Yang; C.J. Qian; X. Shi; H.T. Gu; S.B. Lin; Y. Zhang; J. Yang; C.J. Qian
06/24/2016
pIRES2-EGFP-TRIM28; Transfection; TRIM28

The bovine TRIM28 gene was amplified from ovary tissue by using RT-PCR. The TRIM28 gene was inserted into the eukaryotic expression vector pIRES2-EGFP and transfected into bovine fetal fibroblasts by using Lipofectamine 3000. TRIM28 mRNA and protein were detected by fluorescence microscope and western blotting. The results showed that the full length of TRIM28 was cloned and pIRES2-EGFP- ... more

X. Ma; Z.C. Zhai; M.L. Zhang; B.H. Song; Y.R. Zhu; S.B. Yang; X.Q. Dong; L.Y. Su; C.F. Wang; H.X. Ma; W.M. Luan; X. Ma; Z.C. Zhai; M.L. Zhang; B.H. Song; Y.R. Zhu; S.B. Yang; X.Q. Dong; L.Y. Su; C.F. Wang; H.X. Ma; W.M. Luan
06/10/2016
Aldehyde dehydrogenase 2; Bioconversion; Nitric oxide; Nitroglycerin tolerance; Oxidative damage; Transfection

Medical nitroglycerin (glyceryl trinitrate, GTN) use is limited principally by tolerance typified by a decrease in nitric oxide (NO) produced by biotransformation. Such tolerance may lead to endothelial dysfunction by inducing oxidative stress. In vivo studies have demonstrated that aldehyde dehydrogenase 2 (ALDH2) plays important roles in GTN biotransformation and tolerance. ... more

X.Y. Hu; Q. Fang; D. Ma; L. Jiang; Y. Yang; J. Sun; C. Yang; J.S. Wang; X.Y. Hu; Q. Fang; D. Ma; L. Jiang; Y. Yang; J. Sun; C. Yang; J.S. Wang
04/26/2016
Antimutagenesis; Apoptosis; Campomanesia adamantium; Micronucleus; Mutagenesis

Campomanesia adamantium (Cambess.) O. Berg. is originally from Brazil. Its leaves and fruits have medicinal properties such as anti-inflammatory, antidiarrheal and antiseptic properties. However, the mutagenic potential of this species has been reported in few studies. This study describes the mutagenic/antimutagenic, splenic phagocytic, and apoptotic activities of C. ... more

M.D. Martello; N. David; R. Matuo; P.C. Carvalho; S.D. Navarro; A.C.D. Monreal; A.L. Cunha-Laura; C.A.L. Cardoso; C.A.L. Kassuya; R.J. Oliveira; M.D. Martello; N. David; R. Matuo; P.C. Carvalho; S.D. Navarro; A.C.D. Monreal; A.L. Cunha-Laura; C.A.L. Cardoso; C.A.L. Kassuya; R.J. Oliveira; M.D. Martello; N. David; R. Matuo; P.C. Carvalho; S.D. Navarro; A.C.D. Monreal; A.L. Cunha-Laura; C.A.L. Cardoso; C.A.L. Kassuya; R.J. Oliveira
04/25/2016
Apoptosis; Ndfipl; Parkinson’s disease; Ubiquitination

The aim of the current study was to examine the protective effects and mechanisms of Ndfipl on neurocytes in an experimental in vitro Parkinson’s disease model induced by MPP+. The cell model was developed with dominant negative expression and suppressed expression of Ndfipl by means of transient transfection of Ndfipl-dominant negative and -inhibitory vectors. In total, four ... more

L.F. Xing; H.P. Guo; D.T. Wang; L.H. Sun; S.Y. Pan; L.F. Xing; H.P. Guo; D.T. Wang; L.H. Sun; S.Y. Pan; L.F. Xing; H.P. Guo; D.T. Wang; L.H. Sun; S.Y. Pan
04/07/2016
Apoptosis; Autophagy; Beclin-1; Colorectal cancer; MicroRNA-30a; Prognosis

We investigated the associations between Beclin-1 and microRNA-30a (miR-30a) expression and the severity and treatment response in colorectal cancer (CRC). Our sample size consisted of 139 CRC patients who were treated with surgery alone. Immunohistochemistry was used to investigate the expression and prognostic significance of Beclin-1 in CRC, while the weak expression of Beclin-1 in ... more

L. Liu; T. Meng; Q.S. Wang; H.Z. Jin; Z.Q. Sun; B. Jin; F. Fang; H.J. Wang; L. Liu; T. Meng; Q.S. Wang; H.Z. Jin; Z.Q. Sun; B. Jin; F. Fang; H.J. Wang; L. Liu; T. Meng; Q.S. Wang; H.Z. Jin; Z.Q. Sun; B. Jin; F. Fang; H.J. Wang
04/04/2016
Apoptosis; Gastric cancer; miR-451; MMP-2; Proliferation; Propofol

Propofol is an extensively used intravenous anesthetic agent. The aim of the present study was to evaluate the effects of propofol on the behavior of human gastric cancer cells and the molecular mechanisms associated with this activity. The effects of propofol on proliferation and apoptosis in the SGC-7901 gastric cancer cell line were detected by an MTT assay and measurement of caspase-3 ... more

Z. Peng; Y. Zhang; Z. Peng; Y. Zhang; Z. Peng; Y. Zhang
04/04/2016
Apoptosis; Colorectal cancer; Invasion; MiR-874; Prognosis; Proliferation
X.J. Wang; M. Xia; W.P. Bi; X.J. Wang; M. Xia; W.P. Bi; X.J. Wang; M. Xia; W.P. Bi; X.J. Wang; M. Xia; W.P. Bi

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