Research Article

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Deep RNA sequencing (RNA-seq) provides a practical and inexpensive alternative for exploring genomic data in non-model organisms. The functional annotation of non-model mammalian genomes, such as that of goats, is still poor compared to that of humans and mice. In the current study, we performed a whole transcriptome analysis of an intestinal mucous membrane lymph node to comprehensively ... more

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Lactation; PCR; Serology; Sheep; Toxoplasmosis

The objective of this study was to verify whether Toxoplasma gondii is excreted in the milk of naturally infected sheep. In order to accomplish this, 275 lactating ewes were used; these were bred extensively in 17 estates distributed across nine cities. Polymerase chain reaction amplification was used to detect T. gondii DNA in milk samples, and the indirect ... more

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Different tail type; FAS; HSL; mRNA and protein expression; PPARγ; Sheep

Tail fat content affects meat quality, and it varies in different sheep breeds. Theoretically, lipid metabolism contributes to variation in tail fat content. Tail length, tail width, and tail girth were measured in live Tong sheep (with both short fat tail and long fat tail), Shaanbei fine wool sheep (long thin tail), Tan sheep (short fat tail), Kazakh sheep (hip fat tail), and Tibetan sheep ( ... more

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Antimicrobial peptides; Innate immunity; Sheep; Sheep beta defensin-2; Single nucleotide polymorphism

The northern region of Brazil produces a large number of sheep, with Pará being the largest sheep breeding state in the region. In the Amazon region, livestock production is a challenge due to the high diversity of pathogens affecting humans and animals. Defensins are antimicrobial peptides acting as a first barrier against micro-organisms and present high variation in different organisms ... more

B.B. Souza; E.M. Barbosa; J.S.N. Azevedo; J.E.G. Campelo; L.F.S. Rodrigues; L.M.L. Pinheiro; S.C.B. Silva; A.S. Schierholt; P.H. Souza; E.C. Gonçalves; S. Filho
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Animals; Apoptosis; Cells, Cultured; Chorionic Gonadotropin; Dose-Response Relationship, Drug; Female; Gene Expression Regulation; Gonadotropins, Equine; Horses; In Vitro Oocyte Maturation Techniques; Oocytes; Receptors, FSH; Receptors, LH; Receptors, LHRH; Sheep

We assessed the effects of equine chorionic gonadotropin (eCG) on oocyte in vitro maturation (IVM), apoptosis, and follicle-stimulating hormone receptor (FSHR), luteinizing hormone receptor (LHR), and gonadotropin-releasing hormone receptor (GnRHR) expression and mRNA levels. Cumulus-oocyte complexes (COCs) were recovered from sheep ovaries and pooled in groups, before being cultured in IVM ... more

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Animals; Gene expression profiling; Gene Expression Regulation; Genetic markers; Oligonucleotide Array Sequence Analysis; Phenotype; Quantitative trait loci; Sheep; Wool

Fiber diameter is a useful indicator of wool traits and it is the main determinant of wool quality and value. A comparative study was conducted to analyze the abundance and expression of 13 candidate genes using expression profile microarray analysis and to identify novel molecular markers associated with wool traits to provide a molecular basis for improving wool quality in sheep. Genes ... more

Y.Z. Tian; T. Usman; K.C. Tian; J. Di; X.X. Huang; X.M. Xu; H. Tulafu; W.W. Wu; X.F. Fu; Y. Bai; M. Tuerxun; A. Maimaiti
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Copy number of variations; Insertions / deletions; Ovis aries; Sheep; SNPs

We report the whole ram genome of Jordanian Awassi (Ovis aries), screened using the power of Illumina HiSeq sequencing technology, providing insights into the ram's genomic structure. Generated data will help in the assessment of naturally occurring genetic variation and population structure of this sheep breed using different structure variation markers. ... more

N. Haddad; H. Migdadi; R. Al-Atiyat; K. Jawasre; S. Awabdeh; W. Obeidat; R. AlOmari; M. Aldamra; H. Ababneh; M.J. Tabbaa; M. Brake; M. Farooq
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Bias curve; Isoform expression level; Multi-sample; RNA-seq; Structured sparse regression

With the rapid development of next-generation high-throughput sequencing technology, RNA-seq has become a standard and important technique for transcriptome analysis. For multi-sample RNA-seq data, the existing expression estimation methods usually deal with each single-RNA-seq sample, and ignore that the read distributions are consistent across multiple samples. In the current study, we ... more

L. Zhang; X.J. Liu; L. Zhang; X.J. Liu