Research Article

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The mRNA expression levels of key genes (Smads, MSTN, and MyoG) in the TGF-β/Smad signaling pathway in Hu sheep at different growth stages (2 days, 2 months, and 6 months of age) and in different skeletal muscles (longissimus dorsi muscle and soleus muscle) and different genders were detected; and correlation of the Smad family (Smad2 ... more

Q.Z. Wang; R. Su; X.Y. Lv; W. Gao; L. Chen; J.J. Bao; J.R. Yu; L.H. Wang; W. Sun; Q.Z. Wang; R. Su; X.Y. Lv; W. Gao; L. Chen; J.J. Bao; J.R. Yu; L.H. Wang; W. Sun
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Hu sheep; Maternal behavior; PRLR gene; SNP

The prolactin receptor gene (PRLR) plays an essential role in maternal behavior. The aim of the study was to detect PRLR mutations in exon 10, using a polymerase chain reaction-single stranded conformation polymorphism method, and to determine the association between mutations in this region with maternal behavior traits in Chinese Hu sheep. Polymorphisms were detected ... more

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Hu sheep; mRNA expression; NR5A2; Reproductive performance; Single nucleotide polymorphism

NR5A2 has been implicated in processes as diverse as steroidogenesis, cellular proliferation, ovarian follicular development, ovulation, and fertility in mammals. However, data about the relationship between NR5A2 and prolificacy in mammals are lacking. In the present study, we identified and characterized NR5A2 of Hu sheep, and investigated the correlation ... more

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Gene expression; Hu sheep; MSTN; Smads; YAP1

The temporal and spatial patterns of Smad and Yes-associated protein 1 (YAP1) expression were investigated in skeletal muscle (gastrocnemius muscle and extensor digitorum longus) at different growth stages (2 days old, 2 and 6 months old) in Hu sheep. Smads were differentially expressed in sheep skeletal muscle, with high expression in the gastrocnemius muscle ... more

W. Gao; R. Su; W. Sun; Q.Z. Wang; X.Y. Lv; J.J. Bao; J.R. Yu; L.H. Wang; H.H. Musa; L. Chen; W. Gao; R. Su; W. Sun; Q.Z. Wang; X.Y. Lv; J.J. Bao; J.R. Yu; L.H. Wang; H.H. Musa; L. Chen
08/05/2016
Hair follicle; Hu sheep; Patterns

The aim of this study was to detect candidate genes for the development of hair follicles in the Hu sheep breed. Seven genes have been detected in large, medium, and small wave follicles of Hu sheep using gene chip technology. The histological features of the follicles of newborn Hu-lambs were combined with fluorescence quantitative PCR technology to detect the correlation between the ... more

X.Y. Lv; R. Ni; W. Sun; R. Su; H.H. Musa; J.F. Yin; Q.Z. Wang; W. Gao; L. Chen; X.Y. Lv; R. Ni; W. Sun; R. Su; H.H. Musa; J.F. Yin; Q.Z. Wang; W. Gao; L. Chen
04/25/2016
Gene expression; Habitual abortion; MCP2; TLR4

Habitual abortion is associated with the altered expression of multiple genes. This study was carried out to investigate the relationship between expression of Toll-like receptor 4 (TLR4) and monocyte chemotactic protein 2 (MCP2 or CCL8) and habitual abortion. This was done by detecting and comparing their relative expression in peripheral blood and placental villi of patients and healthy ... more

X.P. Li; L.N. Song; L.P. Tian; Y.S. Zhang; X.P. Li; L.N. Song; L.P. Tian; Y.S. Zhang; X.P. Li; L.N. Song; L.P. Tian; Y.S. Zhang
04/25/2016
Exopalaemon carinicauda; Gene expression; Internal control; Ovarian development; β-actin

Actin is a highly conserved protein that is found in all eukaryotic cells, and has been widely used as an internal control gene in gene expression studies. In this study, we cloned an actin gene (named Ecβ-actin) from Exopalaemon carinicauda and determined its expression levels. The full-length cDNA of Ecβ-actin was 1335 bp long ... more

J.P. Liang; Y. Wang; Q.Q. Ge; J.T. Li; P. Liu; J. Li; G.X. Nie; J.P. Liang; Y. Wang; Q.Q. Ge; J.T. Li; P. Liu; J. Li; G.X. Nie; J.P. Liang; Y. Wang; Q.Q. Ge; J.T. Li; P. Liu; J. Li; G.X. Nie
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avTOR; Chickens; Feed efficiency; Gene expression

Avian target of rapamycin (avTOR) is a highly conserved serine-threonine kinase that serves as an intracellular energy and nutrient sensor and regulates cell division, growth, and apoptosis. The role of avTOR in mediating feed intake and growth in poultry is unknown. We studied avTOR signaling activities in duodenum and liver tissues at days 35 and 42 in chickens divergently selected for ... more

J. Lee; S.E. Aggrey; J. Lee; S.E. Aggrey
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Gene expression; IGF1R; Pyometra; Real-time quantitative PCR; Reference genes

Real-time quantitative polymerase chain reaction (RT-qPCR) is usually employed in gene expression studies in veterinary research, including in studies on canine pyometra. Canine pyometra is a common clinical disease in bitches. When using RT-qPCR, internal standards, such as reference genes, are necessary to investigate relative gene expression by quantitative measurements of mRNA levels ... more

M. Du; X. Wang; Y.W. Yue; P.Y. Zhou; W. Yao; X. Li; X.B. Ding; X.F. Liu; H. Guo; W.Z. Ma; M. Du; X. Wang; Y.W. Yue; P.Y. Zhou; W. Yao; X. Li; X.B. Ding; X.F. Liu; H. Guo; W.Z. Ma
05/25/2016
Electron transport chain; Energy metabolism; Gene expression; Oxidative phosphorylation

This study was carry out to evaluate mRNA expression of mitochondrial cytochrome c oxidase III in the Pectoralis superficialis muscle of 28-day-old quails fed diets containing 0, 8, and 12% glycerol. Total RNA was extracted (N = 10) and cDNA was amplified using specifics primers for qRT-PCR. Feed efficiency and feed intake were evaluated. COX III mRNA expression in breast muscle was ... more

S.C.C. Silva; E. Gasparino; E. Batista; F. Tanamati; A.P.D. Vesco; B. Lala; D.P. de Oliveira; S.C.C. Silva; E. Gasparino; E. Batista; F. Tanamati; A.P.D. Vesco; B. Lala; D.P. de Oliveira

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