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X.K. Xing; H.Y. Wu; H.G. Feng; Z.Q. Yuan; X.K. Xing; H.Y. Wu; H.G. Feng; Z.Q. Yuan; X.K. Xing; H.Y. Wu; H.G. Feng; Z.Q. Yuan
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The purpose of this study was to screen the key genes and pathways of renal cell carcinoma (RCC) and lay the foundation for its diagnosis and therapy. Microarray data of normal subjects and RCC patients at different stages of disease were used to screen differentially expressed genes (DEGs). Based on the DEGs in the four disease stages, four co-expression networks were constructed using ... more

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ELISA; Immunohistochemistry; Polyclonal antibody; Porcine β-defensin 2; Western blotting

Porcine β-defensin 2 (pBD2) is an antimicrobial peptide in pigs that plays an important role in the immune system by preventing bacterial invasion. To produce an anti-pBD2 antibody, which is not commercially available, we expressed and purified a soluble, his-tagged version of pBD2 (his-pBD2). Purified pBD2 was injected into New Zealand white rabbits to generate polyclonal antiserum. Anti ... more

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CK19 mRNA; KS1/4 mRNA; LUNX mRNA; Micrometastases; Non-small cell lung cancer; RT-PCR

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GA733-1/-2/-3 genes have been detected in various types of cancer, although their role has not been fully clarified. GA733-2 and GA733-1 have been correlated with lymph node metastases in laryngeal cancer and liver metastases, respectively. Only a few studies have elucidated the mechanisms regulating GA733-1/-2 expression and their effect on colorectal cancer. ... more

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Fermentation is an important industrial process for microbial metabolite development and has wide applications in various fields. Aspergillus is the most important genus of fungi used for the production of microbial enzymes such as cellulase. The Aspergillus genome encodes various cellulolytic enzymes. In this study, we assayed the gene expression ... more

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Detection via PCR is a fast, sensitive, and highly specific method. However, the cost for testing through this technique is quite high, mainly because of the costs of the kits that are used. We looked for the best cost-effective alternative for Dengue virus (DENV) detection via PCR through the evaluation, optimization, and comparison of RT-PCR (Reverse ... more

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