Research Article

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03/11/2016
Cloning; EFNB2; Expression profiles; Pig

Ephrin-B2 (EFNB2) is a signaling molecule that plays an important role in cell adhesion, proliferation, and migration in humans. However, little is known about this molecule in pigs. In order to investigate whether EFNB2 is associated with the skeletal muscle in pigs, we cloned the full-length cDNA of EFNB2 (GenBank accession No. KF500033) from the longissimus dorsi ... more

D. Xu; H.M. Ma; D. Xu; H.M. Ma
02/05/2016
5-HT; Black tiger shrimp (Penaeus monodon); Cloning; PmMAT1; Quantitative real-time PCR

MAT1 (ménage à trois 1), an assembly factor and targeting subunit of the CDK-dependent kinase (CAK), can regulate the cell cycle, transcription, and DNA repair. This study was intended to investigate the role of MAT1 in the reproductive maturation of black tiger shrimp (Penaeus monodon). In this study, the P. monodon MAT1 (PmMAT1) gene was identified and ... more

Y. Wang; M.J. Fu; C. Zhao; W.Y. Bao; F.L. Zhou; Q.B. Yang; S.G. Jiang; L.H. Qiu; Y. Wang; M.J. Fu; C. Zhao; W.Y. Bao; F.L. Zhou; Q.B. Yang; S.G. Jiang; L.H. Qiu
06/03/2016
Copy number variation; DHRS4 gene cluster; DNA methylation; Natural antisense transcription; Transcriptional regulation

The dehydrogenase/reductase (SDR family) member 4 (DHRS4) gene is copied during mammalian evolution; therefore, while only one DHRS4 gene is expressed in the mouse genome, the gene cluster consists of two (DHRS4 and DHRS4L1) and three (DHRS4, DHRS4L2, and DHRS4L1) copies in chimpanzees and humans, respectively. In this study, ... more

Z. Su; G. Liu; X. Song; B. Liang; X. Chang; D. Huang; Z. Su; G. Liu; X. Song; B. Liang; X. Chang; D. Huang
08/12/2016
Copy number variation; MAP3K5; mRNA expression; Porcine; RACE; Residual feed intake

Mitogen-activated protein kinase kinase kinase 5 (MAP3K5) is essential for apoptosis, proliferation, differentiation, and immune responses, and is a candidate marker for residual feed intake (RFI) in pig. We cloned the full-length cDNA sequence of porcine MAP3K5 by rapid-amplification of cDNA ends. The 5451-bp gene contains a 5'-untranslated region (UTR) (718 bp), a coding region ... more

L. Pu; L.C. Zhang; J.S. Zhang; X. Song; L.G. Wang; J. Liang; Y.B. Zhang; X. Liu; H. Yan; T. Zhang; J.W. Yue; N. Li; Q.Q. Wu; L.X. Wang; L. Pu; L.C. Zhang; J.S. Zhang; X. Song; L.G. Wang; J. Liang; Y.B. Zhang; X. Liu; H. Yan; T. Zhang; J.W. Yue; N. Li; Q.Q. Wu; L.X. Wang
12/29/2015
Cloning; Expression; Heat shock protein 90 (HSP90); Pinctada martensii; Stress

Heat shock protein 90 (HSP90) is an important molecular chaperone required for proper folding of cellular proteins, and thus, it plays an essential role in protecting cells from damage during stress. In this study, an HSP90 cDNA designated PmHSP90 was cloned from the mantle tissue of the pearl oyster Pinctada martensii using reverse transcription polymerase chain ... more

H.Y. Liang; Z.X. Wang; Q.N. Lei; R.L. Huang; Y.W. Deng; Q.H. Wang; Y. Jiao; X.D. Du
12/14/2015
Cadmium; Cloning; Metallothionein-2; Pheretima aspergillum (E. Perrier)

Metallothioneins (MTs) are ubiquitous metal-binding, cysteine-rich proteins, associated with metal accumulation and thus providing protection against toxic heavy metals such as cadmium (Cd). To investigate the mechanisms of enrichment of Cd in the earthworm Pheretima aspergillum, we isolated and cloned metallothionein-2 (MT-2) cDNA (538 bp) from P. aspergillum, analyzed its ... more

L. Gong; W. Li; J. Li; W.E. Li; W.R. Wu; L.W. Yu
12/07/2015
Cloning; Expression profiles; Pig; Single nucleotide polymorphism; SLC13A5

Solute carrier family 13 (sodium-dependent citrate transporter member 5, SLC13A5) gene has been recently found to play an important role in intramuscular fat content in pigs. In this study, the full-length cDNA of porcine SLC13A5 was obtained from the longissimus dorsi muscle of Shaziling pigs, using the rapid amplification of cDNA ends technique. Full-length porcine ... more

L.Y. Wang; J. Jiang; H.M. Ma
11/25/2015
Cloning; Penta D allele; Sequencing; Short tandem repeat; Zhuang nationality

Kinship testing of a father and his son from Guangxi, China, the location of the Zhuang minority people, was performed using the PowerPlex® 18D System with a short tandem repeat typing kit. The results indicated that both the father and his son had an off-ladder allele at the Penta D locus, with a genetic size larger than that of the maximal standard allelic ladder. To further identify ... more

Y.L. Yang; J.G. Wang; D.X. Wang; W.Y. Zhang; X.J. Liu; J. Cao; S.L. Yang
10/19/2015
Aquaporin; Bioinformatics analysis; Cloning; Trichinella spiralis

Vaccination as a preventative strategy against Trichinella spiralis infection is an ongoing effort, although no ideal vaccine candidates have been identified until now. Identification of more effective antigens that have a role in essential life stages of the parasite and that may be effective vaccine candidates is therefore of importance. In the present study, we identified a ... more

J.M. Cui; N.Z. Zhang; W.H. Li; H.B. Yan; B.Q. Fu
10/16/2015
Cloning; Gene expression; Insulin-like growth factor-binding protein; Nanjiang brown goat

Insulin-like growth factor-binding proteins (IGFBPs) play a key role in modulating insulin-like growth factors (IGFs), and are considered candidate genes for growth traits in livestock. In this study, we identified the complete coding sequences of IGFBP-1 to -6 in the Nanjiang brown goat, and assessed gene tissue expression patterns by quantitative polymerase chain ... more

S.Y. Zhan; L. Chen; L. Li; L.J. Wang; T. Zhong; H.P. Zhang

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