Research Article

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06/13/2005
Fibroblasts; Liposomes; Livestock cells; Transfection

The development of an efficient transfection system in livestock cells is an important step towards investigating gene transfer and the functioning and production of transgenic animals. Important factors involved in cationic liposome mediated gene transfer were evaluated through in vitro transfection of bovine, caprine and ovine fibroblast cells. Transfection of plasmid DNA complexes of ... more

R.R. Oliveira; D.M. de Carvalho; S. Lisauskas; E. Mello; G.R. Vianna; M.A.N. Dode; R. Rumpf; F.J.L. Aragão; E.L. Rech; E.L. Rech
03/03/2005
Assisted reproduction technology; Bovine; Fibroblasts; Transfection; Transgenic animals

An association of two techniques, nuclear transfer (NT), and transfection of somatic animal cells, has numerous potential applications and considerable impact, mainly in agriculture, medicine, pharmacy, and fundamental biology. In addition, somatic cell nuclear transfer is the most efficient alternative to produce large transgenic animals. We compared in vitro and in vivo ... more

L.T. Iguma; L.T. Iguma; E.O. Melo; M.M. Franco; I. Pivato; G.R. Vianna; R.V. Sousa; M.A.N. Dode; F.J.L. Aragão; E.L. Rech; E.L. Rech; R. Rumpf; S.F.C. Lisauskas
01/24/2014
Fibroblasts; High glucose; Interleukin-1β; Matrix metalloproteinase-2; Mineralocorticoid receptor antagonists

The aim of this study was to determine the influence of high glucose (HG) and interleukin (IL)-1β on human cardiac fibroblast (HCF) functions, and to evaluate the effects of eplerenone in these responses. HCFs were cultured in normal or HG media in the absence or presence of IL-1β and/or eplerenone. We assessed matrix metalloproteinase-2 (MMP-2) activity in the supernatant by in-gel ... more

J.F. Chi; H. Uzui; H.Y. Guo; T. Ueda; J.D. Lee
3/8/2017
Adult; Aged; Cytokines; Endothelial cells; Female; Fibroblasts; Humans; Macrophages; male; Mast Cells; Middle Aged; Periapical Diseases; Periapical Granuloma; Periapical Tissue; Radicular Cyst; Stem Cell Factor

Stem cell factor (SCF), an important stem cell cytokine, has multiple functions. Fibroblasts (FBs), mature mast cells, endothelial cells (ECs), and eosinophil granulocytes can produce SCF in the inflammatory process. Therefore, we aimed to observe SCF expression in FBs, ECs, and macrophages (MPs) in periapical tissues in human chronic periapical disease and investigate the effects of cells ... more

S.Q. Shen; R. Wang; S.G. Huang
10/05/2017
ASCS; Blood bioactive scaffolds; Cell therapy; Fibroblasts; Scaffolds

This study aimed to compare the performance of bloodderived scaffolds with a well-known and accepted scaffold, chitosan, in maintaining cell cultures of ASCs and fibroblasts for future wound healing applications. Cells were characterized, immunophenotyped and cultivated into the following scaffolds: 1) Chitosan (CH, control), 2) Platelet gel (PG), 3) Chitosan ... more

H.Caputo Nunes; R.Rossi Ferreira; A.Carolina P. Pasian; A. Moroz; B. Martinucci; M.Janegitz A. Valeri; S.Alexandre Santos; Hde Souza Vieira; S.Luis Felisbino; E. Deffune; F.Karina Delella
12/29/2015
Expression profile; GRAS; Jatropha curcas L.; Phylogeny; Physic nut

GRAS proteins play vital roles in plant growth and development. Physic nut (Jatropha curcas L.) was found to have a total of 48 GRAS family members (JcGRAS), 15 more than those found in Arabidopsis. The JcGRAS genes were divided into 12 subfamilies or 15 ancient monophyletic lineages based on the phylogenetic analysis of GRAS proteins from both flowering and ... more

Z.Y. Wu; P.Z. Wu; Y.P. Chen; M.R. Li; G.J. Wu; H.W. Jiang
12/22/2015
Expression profile; GAS6; Immunohistochemistry; Pig; SNP

The growth arrest-specific 6 gene (GAS6) is a member of the family of plasma vitamin K-dependent proteins, which are able to bind to phospholipids using an N-terminal gamma-carboxyglutamic acid domain. A recent report has demonstrated that the GAS6 gene can promote fat deposition and is associated with an increased number of fat cells in mice. In order to investigate ... more

D. Xu; J. Jiang; J. He; C.Q. He; H.M. Ma
10/19/2015
CDC16; Chromosomal localization; Expression profile; Immunohistochemistry; Pig

Cell division cycle16 (CDC16) is a core component among the eight protein subunits of the anaphase-promoting complex (APC). APC is a cyclin degradation system that governs the exit of cells from mitosis. Not much information is available for CDC16 in pig. In this study, a 2284-bp cDNA of porcine CDC16 was obtained by rapid amplification of cDNA ends (RACE). Porcine ... more

D. Xu; J. Jiang; H. Ma