Research Article

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Spontaneous mutations are a common phenomenon, occurring in both germ-line and somatic genomes. They may have deleterious consequences including the development of genetic disorders or, when occurring in somatic tissues, may participate in the process of carcinogenesis. Similar to many mutational hotspots, the G1138A mutation in the fibroblast growth factor receptor 3 (FGFR3 ... more

P.L. Reddy; R.P. Grewal
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Cyclin D1; Multiple myeloma; Single nucleotide polymorphism

Cyclin D1 is an important cell cycle regulator implicated in the pathogenesis of many cancer types. In particular, translocation and overexpression of cyclin D1 are common events in multiple myeloma (MM), suggesting that it may drive the initiation and progression of this malignancy. However, the association between genetic polymorphisms of cyclin D1 and the risk for developing MM remains ... more

S. Wang; Y. Huang; R. Su; Z. Fang; M. Han
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Biomarker; Diagnosis; HCC; miR-21; Prognosis

The diagnostic and prognostic value of miR-21 has been examined for hepatocellular carcinoma (HCC), with inconsistent results. Present meta-analysis summarized the diagnostic accuracy and the predictive role for survival of miR-21 in patients with HCC. All eligible studies were searched using PubMed, EMBASE, and Chinese National Knowledge Infrastructure (CNKI) databases up to October 2014 ... more

S.R. Yan; Z.J. Liu; S. Yu; Y.X. Bao
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Esophageal cancer; Esophageal cancer cell lines; miR-21; Transfection

The aim of this study was to investigate the expression of miR-21 in esophageal cancer and the impact of miR-21 on apoptosis, invasion, and the expression of target genes in esophageal cancer cells. Fluorescence quantitative polymerase chain reaction analysis was used to detect the expression of miR-21 in human esophageal tissues, adjacent tissues, and an esophageal cancer cell line (TE- ... more

S.W. Wen; Y.F. Zhang; Y. Li1; Z.X. Liu; H.L. Lv; Z.H. Li; Y.Z. Xu; Y.G. Zhu; Z.Q. Tian
05/28/2019
Glioblastoma; ionizing radiation; MicroRNAs; miR-21; Neurospheres; Temozolomide

Glioblastomas are the most common primary malignant brain tumors, and despite current advances in therapy, they are still extremely resistant to treatment. The tumorigenic potential of glioblastomas is due to a subpopulation of neoplastic cells, called cancer stem cells (CSCs), which in culture form a suspension cluster called neurospheres. Their properties include ... more

A.R. Rodrigues; F.S.L. Neto; L.G. Lourenço; F.A. Trevisan; M.L.A. Cirino; B. Nery; F.M. Peria; G. Pereira-da-Silva; M.F.G.S. Tazima; L.F. Tirapelli; D.G. Tiezzi; C.G.Carlotti Junior; D.P.C. Tirapelli
07/31/2015
Lactation; PCR; Serology; Sheep; Toxoplasmosis

The objective of this study was to verify whether Toxoplasma gondii is excreted in the milk of naturally infected sheep. In order to accomplish this, 275 lactating ewes were used; these were bred extensively in 17 estates distributed across nine cities. Polymerase chain reaction amplification was used to detect T. gondii DNA in milk samples, and the indirect ... more

deSantana Rocha; R.Lde Sousa Moura; B.M. Maciel; L.A. Guimarães; H.N.S. O’dwyer; A.D. Munhoz; G.R. Albuquerque
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Genetic relationship; PCR; Polymorphism; RAPD

The artichoke (Cynara scolymus L.) is an important food and medicinal crop that is cultivated in Mediterranean countries. Morphological characteristics, such as head shape and diameter, leaf shape, and bract shape, are mainly affected by environmental conditions. A molecular marker approach was used to analyze the degree of polymorphism between artichoke hybrid lines. The degree ... more

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Forestry species; Genetic parameters; PCR

Nectandra megapotamica (Spreng.) Mez. is a tree species that naturally occurs in the Atlantic Forest, Brazil. The aim of this study was to evaluate the genetic diversity and structure of a natural population of 12 N. megapotamica individuals using random amplified polymorphic DNA markers. Eleven primers were used in this study, producing 81 bands, of which 98.99% were ... more

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Fragile X syndrome; Intellectual disability; PCR; PCR-P; PCR-T

The aim of this study was to validate the molecular genetic diagnosis of patients suspected of Fragile X Syndrome (FXS) in the Laboratory of Human Cytogenetics and Molecular Genetics (LaGene) of the Department of Health of the State of Goiás, using polymerase chain reaction (PCR). Thirty-five patients referred by public health doctors to LaGene, indicating clinical diagnosis of FXS, were ... more

A.P. Amancio; C.Ade O. Melo; deM. Vieira; L.B. Minasi; deM. e Silva; C.C. da Silva; A.D. da Cruz
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High-throughput; Homologous recombination; In vivo cloning; PCR

In this study, we optimized a restriction-ligation-free (RLF) method to save time and cost of constructing multiple plasmids with the same gene insert, and examined the efficacy of RLF on high-throughput multi-plasmid cloning. This method utilizes the precise DNA repair and recombination systems within Escherichia coli, which allows to bypass the in vitro restriction and ... more

Y. Wang; Y. Liu; J. Chen; M.J. Tang; S.L. Zhang; L.N. Wei; C.H. Li; D.B. Wei

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