Research Article

Related GMR Articles

11/03/2016
Expression analysis; Gene cloning; Mussel; Mytilus coruscus; Wnt7b gene

To understand the potential functions of Wnt7b in different developmental stages and adult tissues of the mussel Mytilus coruscus, the Wnt7b gene was cloned using the rapid amplification of cDNA ends technique. The full-length Wnt7b gene was 1910 bp long, with a 1059-bp open reading frame encoding 352 amino acids. The amino acid sequence of the M. ... more

Y.F. Xu; X. Liang; Y.R. Chen; Y.F. Li; J.L. Yang; Y.F. Xu; X. Liang; Y.R. Chen; Y.F. Li; J.L. Yang
08/10/2015
Callus; Gene cloning; Isoflavone synthase; Sequence analysis; Trifolium pretense

The aim of this study was to clone the isoflavone synthase (IFS) gene and establish the recombinant Minshan Trifolium pratense. The IFS gene was cloned from the callus of Minshan T. pratense using reverse transcription-polymerase chain reaction. The plant expression vector pRI101-AN-IFS was constructed and introduced into Agrobacterium tumefaciens strain LBA4404, and ... more

H.H. Hu; C.Q. Jing; R. Liu; W.D. Li; H.G. Feng
08/10/2015
Bambusa oldhamii; Gene cloning; Sequence analysis; TERMINAL FLOWER1; Transgenic Arabidopsis thaliana

TERMINAL FLOWER1 (TFL1) homologous genes play major roles in maintaining vegetative growth and inflorescence meristem characteristics in various plant species; however, to date, the function of the bamboo TFL1 homologous gene has not been described. In this study, a TFL1 homologous gene was isolated from Bambusa oldhamii and designated as BoTFL1- ... more

H.Y. Zeng; Y.T. Lu; X.M. Yang; Y.H. Xu; X.C. Lin
12/28/2015
Gene cloning; Hami melon; Real-time fluorescence quantitative PCR; Superoxide dismutase

Primers were designed according to the Cu/Zn-SOD gene sequences of cloned Cucurbits plants (cucumbers and watermelons) available in NCBI. Total RNA from Hami melon pulp was used as a template. Following RT-PCR amplification, a 403-bp fragment of the Hami melon Cu/Zn-SOD gene was obtained. According to alignment in BLAST and phylogenetic tree analysis, the cloned gene fragment was ... more

C.H. Shan; F.X. Tang; W. Chen; W.R. Ma
12/22/2015
Eruca vesicaria subsp sativa; Gene cloning; Nicotianamine synthase

Nicotianamine (NA) is a ubiquitous metabolite in plants that bind heavy metals, is crucial for metal homeostasis, and is also an important metal chelator that facilitates long-distance metal transport and sequestration. NA synthesis is catalyzed by the enzyme nicotianamine synthase (NAS). Eruca vesicaria subsp sativa is highly tolerant to Ni, Pb, and Zn. In this study, a ... more

B.L. Huang; C. Cheng; G.Y. Zhang; J.J. Su; Y. Zhi; S.S. Xu; D.T. Cai; X.K. Zhang; B.Q. Huang
11/30/2015
Barley; Drought stress; Gene cloning; Gene expression; HbSINA4; Hordeum vulgare

Hulless barley is an important crop cereal in Tibetan, China. Drought is a major abiotic stress in barley production. In this study, we cloned the drought-related HbSINA4 gene from the variety ‘Himalaya 10’ and analyzed its expression patterns under different drought and rehydration conditions. The cDNA of HbSINA4 was 1052 bp long, including an open reading frame of 771 ... more

H.J. Yuan; X.M. Luo; T.S. Nyima; Y.L. Wang; Q.J. Xu; X.Q. Zeng
01/03/2018
Gene cloning; Pistillata-like; qRT-PCR; RACE kit; Sedirea japonica

Members of the pistillata-like (PI-like) subfamily of plant MADS-box genes play multiple roles in regulation of reproductive meristems and the specification of sepal and petal identities. However, the function of PI-like genes in the Sedirea japonica is largely unknown. In this study, a full-length of PI-like gene in Sedirea japonica (designated PI-like, GenBank No ... more

S. Jiang; S. Niu; X. Yuan; F. Liang; S. Xu; M. Wang; B. Cui
08/19/2015
Aleurites moluccana L.; BCCP subunit gene; Expression analysis; Molecular cloning; Sequence analysis

Aleurites moluccana L. is grown as a roadside tree in southern China and the oil content of its seed is higher than other oil plants, such as Jatropha curcas and Camellia oleifera. A. moluccana is considered a promising energy plant because its seed oil could be used to produce biodiesel and bio-jet fuel. In addition, the bark, leaves, and kernels of A. ... more

W.Y. Xuan; Y. Zhang; Z.Q. Liu; D. Feng; M.Y. Luo
07/14/2015
Expression analysis; Gene duplication; Heat shock protein; Tomato

Heat shock protein 90 (Hsp90) is a protein produced by plants in response to adverse environmental stresses. In this study, we identified and analyzed Hsp90 gene family members using a bioinformatic method based on genomic data from tomato (Solanum lycopersicum L.). The results illustrated that tomato contains at least 7 Hsp90 genes distributed on 6 ... more

W.S. Zai; L.X. Miao; Z.L. Xiong; H.L. Zhang; Y.R. Ma; Y.L. Li; Y.B. Chen; S.G. Ye
11/19/2015
Apomixis; Expression analysis; Malus; Somatic embryogenesis receptor-like kinase

This study aims to explore the roles of somatic embryogenesis receptor-like kinase (SERK) in Malus hupehensis (Pingyi Tiancha). The full-length sequences of SERK1 in triploid Pingyi Tiancha (3n) and a tetraploid hybrid strain 33# (4n) were cloned, sequenced, and designated as MhSERK1 and MhdSERK1, respectively. Multiple alignments of amino acid ... more

L.J. Zhang; W.X. Dong; S.M. Guo; Y.X. Wang; A.D. Wang; X.J. Lu