Research Article

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Herein, we describe 34 microsatellite loci developed using an enrichment genomic library for the tree species Hancornia speciosa Gomes (Apocynaceae). Thirty-five individuals were genotyped using 34 primers to analyze the polymorphisms at each locus. The number of alleles per locus ranged from 4 to 20. The average number of alleles was 8.11, and the expected heterozygosity ranged from ... more

A.J.L. Rodrigues; A.T. Yamaguishi; L.J. Chaves; A.S.G. Coelho; J.S. Lima; M.P.C. Telles
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Cerrado; Genetic variability; Hancornia speciosa; Mangabeira; Molecular markers; Transferability frequency

Hancornia speciosa, popularly known as mangabeira, is a fruit tree native to the Brazilian Cerrado that shows great economic potential, due to its multiple uses. Intraspecific classification of this species is difficult because it shows high morphological diversity. An early study of the species reported that there are six botanic varieties that differ morphologically mainly in ... more

C.A. Nogueira; N.B. Stafuzza; T.P. Ribeiro; A.D.L. Prado; I.P.P. Menezes; N. Peixoto; P.J. Gonçalves; L.M. Almeida
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Cerrado; Conservation; Euphorbiaceae; LI-COR; Medicinal plant

Croton antisyphiliticus Mart. is a medicinal plant native to Cerrado vegetation in Brazil, and it is popularly used to treat urogenital tract infections. The objective of the present study was to assess the genetic variability of natural C. antisyphiliticus populations using AFLP molecular markers. Accessions were collected in the states of Minas Gerais, São Paulo, and ... more

T.G. Oliveira; A.M.S. Pereira; J.S. Coppede; S.C. França; L.C. Ming; B.W. Bertoni; T.G. Oliveira; A.M.S. Pereira; J.S. Coppede; S.C. França; L.C. Ming; B.W. Bertoni
12/13/2006
Cerdocyon thous; Cerrado; Chrysocyon brachyurus; Cross-amplification; STR markers

The maned wolf (Chrysocyon brachyurus) and the crab-eating fox (Cerdocyon thous) are two wild-canid species found in the Brazilian Cerrado. We tested cross-amplification and transferability of 29 short tandem repeat primers originally developed for cattle and domestic dogs and cats on 38 individuals of each of these two species, collected in the Emas National Park, ... more

F.M. Rodrigues; M.P.C. Telles; L.V. Resende; T.N. Soares; J.A.F. Diniz-Filho; A.T.A. Jácomo; L. Silveira
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Tomoplagia reticulata and T. pallens are sibling species that are specialists on Eremanthus glomerulatus. Besides adult terminalia, they show slight morphological differences and distinct geographic distributions. Once, however, they were found sympatrically. Using data from allozyme and mtDNA, we examined patterns of intra- and interspecific genetic structure, and ... more

A.G. Abreu; V.N. Solferini
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Cerrado; Pau-papel; Shotgun library; SSR; STR

We isolated and characterized 12 microsatellite loci for Tibouchina papyrus (Melastomataceae), an endangered species with narrow and disjunct range, endemics to a few localities in “cerrado rupestre” from Central Brazil. These microsatellites were obtained by sequencing of a genomic shotgun library for primer design. Leaves from 96 individuals collected in the three known local ... more

M.P.C. Telles; F.P. Peixoto; J.S. Lima; L.V. Resende; R.P. Vianello; M.E.M.T. Walter; R.G. Collevatti
03/01/2011
Cerrado; Dimorphandra mollis; DNA extraction; Metabolic compounds; “Fava d’anta”

Isolation of high-quality DNA from plants, especially plants from the Cerrado, is notoriously difficult because of polysaccharides and secondary compounds produced by plants from this biome. DNA isolation and its quality may be compromised by chemical defenses such as tannins and phenols. Quantitative plant defenses tend to have a cumulative effect, increasing in concentration during ... more

P.A. Moreira; D.A. Oliveira
09/08/2010
Cerrado; Loricariidae; Microsatellite; Shotgun library

We isolated and characterized 10 microsatellite loci in the armored catfish (Hypostomus gymnorhynchus, Loricariidae), using a genomic shotgun library to obtain the repetitive sequences. Twenty-four primers were designed and 14 individuals of H. gymnorhynchus from the Caiapó River, in central Brazil, were genotyped using these primers to analyze the polymorphism at each ... more

M.P.C. Telles; L.V. Resende; R.P.V. Brondani; R.G. Collevatti; M.C. Costa; N.J.Silva Júnior
08/03/2010
Cerrado; Erythrinidae; Microsatellite; Shotgun library

We identified 14 microsatellite loci for the wolf fish, Hoplias malabaricus (Erythrinidae), from a genomic shotgun library. Twenty-five primers were designed, and 48 individuals of H. malabaricus from four localities of northwest Goiás, in central Brazil, were genotyped to characterize the polymorphism at each locus. Fourteen primers amplified clearly interpretable ... more

S.G.C.A. Gondim; L.V. Resende; R.P.V. Brondani; R.G. Collevatti; N.J. Silva-Júnior; R.R. Pereira; M.P.C. Telles
11/27/2013
Alouatta caraya; Assignment test; Brazil; Cerrado; Pedigree; Philopatry

We here investigated the kin structure and pattern of dispersal in the black-and-gold howler monkey (Alouatta caraya, Platyrrhini, Atelidae) based on genotype differences at nine microsatellite loci of 48 individuals from eight social groups along the riparian forest of the Tocantins River, Brazil. The genetic diversity (HE = 0.647) was similar to or higher ... more

R.G. Collevatti; A.C. Souza-Neto; N.J. Silva-Jr.; M.P.C. Telles

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