Research Article

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07/18/2019
Abscisic acid; Coffea arabica; Gibberellic acid; qRT-PCR

During maturation in seeds, metabolic processes dependent on gene expression are controlled by hormones, including abscisic acid and gibberellin. These hormones determine dormancy or the capacity for germination in seeds and may have different expression levels in the endosperm and embryo of coffee seeds. We quantified gene expression in the biosynthetic pathway of ... more

F.Cdos Santos; S.D.V.F. Rosa; S.V.B. Coelho; A.C.S. Clemente; T.B. Fantazzini; C.C. Pereira; F. Caixeta; N.A.B. Fávaris; J.L. Baúte
10/21/2015
NUP98-96; qRT-PCR; R. glutinosa; RACE technology; Sequence analysis

Nucleoporin 98 (NUP98) and nucleoporin 96 (NUP96) are essential components of the nuclear pore complex (NPC) in eukaryote cells. However, there is a lack of available information about complete Rehmannia glutinosa NUP98-96 (rgNUP98-96) sequences. Here, the full-length cDNA sequence of rgNUP96-98 was isolated from R. glutinosa using rapid amplification of cDNA ... more

Y.H. Yang; M.J. Li
12/29/2015
Developmental stages; Four-seed pod mutant; High-throughput Illumina sequencing; Soybean; Transcriptome analysis

Seed number per pod is an important component of yield traits in soybean (Glycine max L.). In 2010, we identified a natural mutant with an increased number of four-seed pods from a soybean variety named ‘Jinong 18’ (JN18). Subsequent observations indicated that the trait was stably inherited. To identify and understand the function of genes associated with this mutant trait, we ... more

Z.Z. Liu; D. Yao; J. Zhang; Z.L. Li; J. Ma; S.Y. Liu; J. Qu; S.Y. Guan; D.D. Wang; L.D. Pan; D. Wang; P.W. Wang
12/11/2015
Aedes aegypti; Isodillapiole; qRT-PCR

The yellow fever mosquito Aedes (Stegomyia) aegypti is the main vector of dengue arbovirus and other arboviruses. Dengue prevention measures for the control of A. aegypti involve mainly the use of synthetic insecticides. The constant use of insecticides has caused resistance in this mosquito. Alternative studies on plant extracts and their products have ... more

V.S. Lima; A.C. Pinto; M.S. Rafael
11/24/2015
cDNA clone; Indigenous Chinese goats; PCR-SSCP; qRT-PCR; RERG gene

The current study aimed to investigate the coding sequence, polymorphisms, and expression of the RERG gene in indigenous Chinese goats. cDNA of RERG, obtained through reverse transcription PCR was analyzed using bioinformatic techniques. Polymorphisms in the exon regions of the RERG gene were identified and their associations with growth traits in three varieties of ... more

M.X. Sui; H.H. Wang; Z.W. Wang
04/07/2016
AHL25; Arabidopsis thaliana; Artificial micro-RNA; AtExpA8; qRT-PCR

Micro-RNAs are cellular components regulating gene expression at the post-transcription level. In the present study, artificial micro-RNAs were used to decrease the transcript level of two genes, AtExpA8 (encoding an expansin) and AHL25 (encoding an AT-hook motif nuclear localized protein) in Arabidopsis thaliana. The backbone of the Arabidopsis ... more

A. Ud-Din; M. Rauf; S. Ghafoor; M.N.K. Khattak; M.W. Hameed; H. Shah; S. Jan; K. Muhammad; A. Rehman; Inamullah; A. Ud-Din; M. Rauf; S. Ghafoor; M.N.K. Khattak; M.W. Hameed; H. Shah; S. Jan; K. Muhammad; A. Rehman; Inamullah; A. Ud-Din; M. Rauf; S. Ghafoor; M.N.K. Khattak; M.W. Hameed; H. Shah; S. Jan; K. Muhammad; A. Rehman; Inamullah
12/15/2017
Immune response; Malaria; Phylogenetic relationship; qRT-PCR; Resistance to insecticides
Anopheles darlingi is the most anthropophilic mosquito related to Plasmodium infection
of malaria, causing significant morbidity and mortality in South America. Pyrethroid chemical has been
used to control mosquitos. We analyzed the expression of trypsin-3 and phosrestin II genes implicated
to feeding and resistance to insecticides, immune response and ... more
Ohse; K. C; Matiolli; C. C; Scarpassa; V. M; Santos; V. S; Tadei; W. P; Rafael; M. S
01/03/2018
Gene cloning; Pistillata-like; qRT-PCR; RACE kit; Sedirea japonica

Members of the pistillata-like (PI-like) subfamily of plant MADS-box genes play multiple roles in regulation of reproductive meristems and the specification of sepal and petal identities. However, the function of PI-like genes in the Sedirea japonica is largely unknown. In this study, a full-length of PI-like gene in Sedirea japonica (designated PI-like, GenBank No ... more

S. Jiang; S. Niu; X. Yuan; F. Liang; S. Xu; M. Wang; B. Cui
02/11/2016
Biomass allocation; Chlorophyll; Coffea arabica; Crop yield; Plant density

The present study was developed to respond to the need for an increase in crop yield in the mountain region of Caparaó (southern Espírito Santo State, Brazil), an area of traditional coffee production. This study aimed to analyze the diversity and characterize the crop yield of genotypes of Coffea arabica L. with potential for cultivation in high plant density systems. In addition ... more

W.N. Rodrigues; M.A. Tomaz; M.A.G. Ferrão; L.D. Martins; T.V. Colodetti; S.V.B. Brinate; J.F.T. Amaral; F.M. Sobreira; M.A. Apostólico; W.N. Rodrigues; M.A. Tomaz; M.A.G. Ferrão; L.D. Martins; T.V. Colodetti; S.V.B. Brinate; J.F.T. Amaral; F.M. Sobreira; M.A. Apostólico
11/21/2016
Coffea arabica; Genetic divergence; Multivariate analysis; Plant breeding

In a coffee plantation of a coffee ‘Acaiá’ cultivar (Coffea arabica), on the Midwest of Minas Gerais in Capitólio city, a different kind of coffee tree was found (1989), possibly due to a mutation. It presented larger leaves and grains than those of conventional coffee trees and was named as “Big Coffee VL.” The aim of this study was to estimate the ... more

J.A. Silva; S.P. Carvalho; A.T. Bruzi; R.J. Guimarães; L.L. Oliveira; L.C. Simões; J.A. Silva; S.P. Carvalho; A.T. Bruzi; R.J. Guimarães; L.L. Oliveira; L.C. Simões

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