Research Article

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Alligator sinensis; cDNA cloning; Homology analysis; Insulin

Insulin is an important endocrine hormone that plays a critical physiological role in regulating metabolism and glucostasis in vertebrates. In this study, the complete cDNA of Alligator sinensis preproinsulin gene was cloned for the first time by reverse transcription-polymerase chain reaction and rapid amplification of cDNA ends methods; the amino acid sequence encoded and ... more

R. Zhang; S.Z. Zhang; E. Li; C. Wang; C.L. Wang; X.B. Wu
Biological evolution; Gossypium raimondii; Phylogeny; Sequence analysis; Synteny; TIFY gene family

The highly conserved TIFY domain is included in the TIFY protein family of transcription factors, which is important in plant development. Here, 28 TIFY family genes were identified in the Gossypium raimondii genome and classified into JAZ (15 genes), ZML (8), PPD (3), and TIFY (2). The normal (TIF[F/Y]XG) motif was dominant in the TIFY ... more

D.H. He; Z.P. Lei; B.S. Tang; H.Y. Xing; J.X. Zhao; Y.L. Jing
Aleurites moluccana L.; BCCP subunit gene; Expression analysis; Molecular cloning; Sequence analysis

Aleurites moluccana L. is grown as a roadside tree in southern China and the oil content of its seed is higher than other oil plants, such as Jatropha curcas and Camellia oleifera. A. moluccana is considered a promising energy plant because its seed oil could be used to produce biodiesel and bio-jet fuel. In addition, the bark, leaves, and kernels of A. ... more

W.Y. Xuan; Y. Zhang; Z.Q. Liu; D. Feng; M.Y. Luo
Callus; Gene cloning; Isoflavone synthase; Sequence analysis; Trifolium pretense

The aim of this study was to clone the isoflavone synthase (IFS) gene and establish the recombinant Minshan Trifolium pratense. The IFS gene was cloned from the callus of Minshan T. pratense using reverse transcription-polymerase chain reaction. The plant expression vector pRI101-AN-IFS was constructed and introduced into Agrobacterium tumefaciens strain LBA4404, and ... more

H.H. Hu; C.Q. Jing; R. Liu; W.D. Li; H.G. Feng
Bambusa oldhamii; Gene cloning; Sequence analysis; TERMINAL FLOWER1; Transgenic Arabidopsis thaliana

TERMINAL FLOWER1 (TFL1) homologous genes play major roles in maintaining vegetative growth and inflorescence meristem characteristics in various plant species; however, to date, the function of the bamboo TFL1 homologous gene has not been described. In this study, a TFL1 homologous gene was isolated from Bambusa oldhamii and designated as BoTFL1- ... more

H.Y. Zeng; Y.T. Lu; X.M. Yang; Y.H. Xu; X.C. Lin
Genotype; Hepatitis B virus; Sequence analysis

Hepatitis B virus genotype C (HBV/C) has the largest number of subgenotypes (C1-C16) that vary with geography and isolates. HBV/C prevails in Southeast Asia (C1, C5-C16), East Asia (C2), Oceania (C3), and Australia (C4). Suitable reference strains for different subgenotypes could greatly facilitate research into HBV/C, but unfortunately they are scarce. We retrieved 974 HBV/C full-length ... more

H.L. Zhu; C.T. Wang; J.B. Xia; X. Li; Z.H. Zhang
cDNA cloning; Cellular localization; Cherax quadricarinatus; Cyclin B; Oogenesis; Spatiotemporal expression profile

Cyclin B is a regulatory subunit of maturation-promoting factor (MPF), which has a key role in the induction of meiotic maturation of oocytes. MPF has been studied in a wide variety of animal species; however, its expression in crustaceans is poorly characterized. In this study, the complete cDNA sequence of Cyclin B was cloned from the red claw crayfish, Cherax quadricarinatus, ... more

L.M. Wang; W.W. Lv; D. Zuo; Z.J. Dong; Y.L. Zhao
cDNA cloning; Chinese perch; Fasting-re-feeding; Gene expression; Myostatin

Myostatin (MSTN) is an important member of the transforming growth factor-β (TGF-β) superfamily and is a muscle growth inhibitor. In the present study, we cloned the Chinese perch MSTN cDNA sequence and analyzed its expression patterns under various conditions. The MSTN full cDNA sequence was 3347 bp long, including an open-reading frame of 1131 bp, which encoded 376 amino acids. Sequence ... more

L. Liu; Y.L. Li; S.D. Xu; K.Z. Wang; P. Wu; W.Y. Chu; X.Q. Wang; L. Liu; Y.L. Li; S.D. Xu; K.Z. Wang; P. Wu; W.Y. Chu; X.Q. Wang
Exopalaemon carinicauda; Gene expression; Internal control; Ovarian development; β-actin

Actin is a highly conserved protein that is found in all eukaryotic cells, and has been widely used as an internal control gene in gene expression studies. In this study, we cloned an actin gene (named Ecβ-actin) from Exopalaemon carinicauda and determined its expression levels. The full-length cDNA of Ecβ-actin was 1335 bp long ... more

J.P. Liang; Y. Wang; Q.Q. Ge; J.T. Li; P. Liu; J. Li; G.X. Nie; J.P. Liang; Y. Wang; Q.Q. Ge; J.T. Li; P. Liu; J. Li; G.X. Nie; J.P. Liang; Y. Wang; Q.Q. Ge; J.T. Li; P. Liu; J. Li; G.X. Nie