Research Article

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07/31/2015
Lactation; PCR; Serology; Sheep; Toxoplasmosis

The objective of this study was to verify whether Toxoplasma gondii is excreted in the milk of naturally infected sheep. In order to accomplish this, 275 lactating ewes were used; these were bred extensively in 17 estates distributed across nine cities. Polymerase chain reaction amplification was used to detect T. gondii DNA in milk samples, and the indirect ... more

deSantana Rocha; R.Lde Sousa Moura; B.M. Maciel; L.A. Guimarães; H.N.S. O’dwyer; A.D. Munhoz; G.R. Albuquerque
12/28/2015
Genetic relationship; PCR; Polymorphism; RAPD

The artichoke (Cynara scolymus L.) is an important food and medicinal crop that is cultivated in Mediterranean countries. Morphological characteristics, such as head shape and diameter, leaf shape, and bract shape, are mainly affected by environmental conditions. A molecular marker approach was used to analyze the degree of polymorphism between artichoke hybrid lines. The degree ... more

M.A. Sharaf-Eldin; A. Al-Tamimi; P. Alam; S.F. Elkholy; J.R. Jordan
12/28/2015
Forestry species; Genetic parameters; PCR

Nectandra megapotamica (Spreng.) Mez. is a tree species that naturally occurs in the Atlantic Forest, Brazil. The aim of this study was to evaluate the genetic diversity and structure of a natural population of 12 N. megapotamica individuals using random amplified polymorphic DNA markers. Eleven primers were used in this study, producing 81 bands, of which 98.99% were ... more

L.S. Costa; L.R.S. Reiniger; B.M. Heinzmann; L.P. Amaral; C.M.L. Serrote
11/19/2015
Fragile X syndrome; Intellectual disability; PCR; PCR-P; PCR-T

The aim of this study was to validate the molecular genetic diagnosis of patients suspected of Fragile X Syndrome (FXS) in the Laboratory of Human Cytogenetics and Molecular Genetics (LaGene) of the Department of Health of the State of Goiás, using polymerase chain reaction (PCR). Thirty-five patients referred by public health doctors to LaGene, indicating clinical diagnosis of FXS, were ... more

A.P. Amancio; C.Ade O. Melo; deM. Vieira; L.B. Minasi; deM. e Silva; C.C. da Silva; A.D. da Cruz
01/22/2016
Correlation; miR-21; Multiple myeloma; PCR

The aim of the present study is to examine the expression level of peripheral mir-21 in multiple myeloma (MM) patients and to determine its clinical significance. MM patients (30), monoclonal gammopathy of undetermined significance (MGUS) patients (14), and normal controls (20) were recruited to determine the serum level of β2-MG, IgA and IgM, IgG, λ, κ, TP, ALB, Hb, LDH, and Ca2+ ... more

J.H. Wang; W.W. Zhou; B.X. Liu; D.L. Man; Z.D. Yang; F.R. Liu; H. Shang; J.H. Wang; W.W. Zhou; B.X. Liu; D.L. Man; Z.D. Yang; F.R. Liu; H. Shang; J.H. Wang; W.W. Zhou; B.X. Liu; D.L. Man; Z.D. Yang; F.R. Liu; H. Shang
12/15/2017
Hemoglobinopathies; PCR; Thalassemias
The hemoglobinopathies are a group of hereditary alterations prevalent in
many parts of the world, but significantly affect the Brazilian population for its abundant
miscegenation. These alterations in structural genes that cause the formation of hemoglobin
variants, and/or regulatory genes, causing thalassemias. Currently, the number of identified
... more
M.H. Lagares; M.S. Rabelo; C.G.B.Dias penna; R.S. Mascarenhas; K.S.F. Silva; K.K.V.O. Moura
05/04/2018
Genetic polymorphisms; PCR; Scientometrics

Scientometrics is a quantitative evaluation of scientific and technological activities. The main objective is to point the number of methodologies used in scientific studies or even the structure of several research centers. This type of metric study belongs to the area of sociology of scientific knowledge. It covers quantitative analyzes of scientific activities ... more

A.M. Barbosa; K.S.F. De Silva; M.H. Lagares; D.A. Rodrigues; J.V.M. Martins; I.R. da Costa; K.K.V.O. Moura
04/06/2018
Electron microscope; GeneBank; ORF 103 gene; PCR; Phylogentic analysis; Sheeppox virus

Sheeppox virus (SPPV) is one of the listed and notifiable disease affect sheep production with major effect on the trade of new breed of sheep. This study was conducted to identify sheep pox by using cell culture, electron microscope (EM) and open reading frame (ORF) 103 gene during an outbreak of local breed of sheep occurred in Sharkia Governorate, Egypt in April ... more

E.B. Abd-Elfatah; M.F. El-Mekkawi; I.M. Bastawecy; E.M. Fawzi
02/26/2019
Bivalve; Diagnostic; PCR; Phylogeny; Protozoan

The parasitic protozoan Perkinsus marinus (Perkinsidae) is known to infect marine bivalves; unfortunately, treatment options are quite limited. The parasite is associated with mass mortalities worldwide and it requires notification to the World Organization for Animal Health (OIE). In Brazil, since the first report of P. marinus infecting ... more

M.P. Leibowitz; F.L. Pereira; C.A.G. Leal; E.A.P. Cunha; V.A.C. Azevedo; H.C.P. Figueiredo
07/31/2019
Bacterium; Brucella; Diagnostics; PCR; Zoonosis

Brucella spp. are the pathogens responsible for brucellosis, which is a cosmopolitan and serious disease for animals and humans. Recent studies have revealed the presence of Brucella DNA in the reproductive tract tissues of buffaloes based on microbiological cultures collected from vaginal swabs. We tested for Brucella abortus DNA in the ... more

H.D.M. Ayala; E.M.M. Monteiro; A.J.S. Souza; S.T.Rolim Filho; E.M. Barbosa; W.G. Vale; S. Filho; W.L.A. Pereira

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