Research Article

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Cadmium; Cloning; Metallothionein-2; Pheretima aspergillum (E. Perrier)

Metallothioneins (MTs) are ubiquitous metal-binding, cysteine-rich proteins, associated with metal accumulation and thus providing protection against toxic heavy metals such as cadmium (Cd). To investigate the mechanisms of enrichment of Cd in the earthworm Pheretima aspergillum, we isolated and cloned metallothionein-2 (MT-2) cDNA (538 bp) from P. aspergillum, analyzed its ... more

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Cloning; Expression profiles; Pig; Single nucleotide polymorphism; SLC13A5

Solute carrier family 13 (sodium-dependent citrate transporter member 5, SLC13A5) gene has been recently found to play an important role in intramuscular fat content in pigs. In this study, the full-length cDNA of porcine SLC13A5 was obtained from the longissimus dorsi muscle of Shaziling pigs, using the rapid amplification of cDNA ends technique. Full-length porcine ... more

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11/25/2015
Cloning; Penta D allele; Sequencing; Short tandem repeat; Zhuang nationality

Kinship testing of a father and his son from Guangxi, China, the location of the Zhuang minority people, was performed using the PowerPlex® 18D System with a short tandem repeat typing kit. The results indicated that both the father and his son had an off-ladder allele at the Penta D locus, with a genetic size larger than that of the maximal standard allelic ladder. To further identify ... more

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Aquaporin; Bioinformatics analysis; Cloning; Trichinella spiralis

Vaccination as a preventative strategy against Trichinella spiralis infection is an ongoing effort, although no ideal vaccine candidates have been identified until now. Identification of more effective antigens that have a role in essential life stages of the parasite and that may be effective vaccine candidates is therefore of importance. In the present study, we identified a ... more

J.M. Cui; N.Z. Zhang; W.H. Li; H.B. Yan; B.Q. Fu
10/05/2015
Cloning; MAO-A; Trophectoderm; X chromosome inactivation

Somatic cell nuclear transfer (SCNT) may affect epigenetic mechanisms and alter the expression of genes related to embryo development and X chromosome inactivation (XCI). We characterized allele-specific expression of the X-linked gene monoamine oxidase type A (MAO-A) in the trophectoderm (TF) of embryos produced by SCNT. Total RNA was isolated from individual biopsies (N = 25), and the ... more

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Cloning; EFNB2; Expression profiles; Pig

Ephrin-B2 (EFNB2) is a signaling molecule that plays an important role in cell adhesion, proliferation, and migration in humans. However, little is known about this molecule in pigs. In order to investigate whether EFNB2 is associated with the skeletal muscle in pigs, we cloned the full-length cDNA of EFNB2 (GenBank accession No. KF500033) from the longissimus dorsi ... more

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02/05/2016
5-HT; Black tiger shrimp (Penaeus monodon); Cloning; PmMAT1; Quantitative real-time PCR

MAT1 (ménage à trois 1), an assembly factor and targeting subunit of the CDK-dependent kinase (CAK), can regulate the cell cycle, transcription, and DNA repair. This study was intended to investigate the role of MAT1 in the reproductive maturation of black tiger shrimp (Penaeus monodon). In this study, the P. monodon MAT1 (PmMAT1) gene was identified and ... more

Y. Wang; M.J. Fu; C. Zhao; W.Y. Bao; F.L. Zhou; Q.B. Yang; S.G. Jiang; L.H. Qiu; Y. Wang; M.J. Fu; C. Zhao; W.Y. Bao; F.L. Zhou; Q.B. Yang; S.G. Jiang; L.H. Qiu
02/05/2016
Cloning; Copy number variation; mRNA expression; Porcine; Signal peptide CUB EGF-like domain-containing protein 3 gene

The signal peptide CUB EGF-like domain-containing protein 3 (SCUBE3) gene is a member of SCUBE gene family and plays important roles in bone cell biology and the determination of limb bone length. In this study, the full-length transcript of porcine SCUBE3 was cloned using reverse transcription-polymerase chain reaction and rapid amplification of cDNA ends. The ... more

X. Liu; L.G. Wang; L.C. Zhang; H. Yan; K.B. Zhao; J. Liang; N. Li; L. Pu; T. Zhang; L.X. Wang; X. Liu; L.G. Wang; L.C. Zhang; H. Yan; K.B. Zhao; J. Liang; N. Li; L. Pu; T. Zhang; L.X. Wang
09/28/2015
Gene expression; Hypoxia; Muscle; Pig

Hypoxia influences many physiological processes, such as respiration, cardiovascular, neurophysiology, and digestion. Skeletal muscle is an important motor organ, which relies on oxygen of oxidation; however, the study of hypoxia in skeletal muscle is lacking. In order to understand the effect of hypoxia on skeletal muscle, we determined the expression level of four hypoxia-related genes ( ... more

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