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The objective of this study was to verify whether Toxoplasma gondii is excreted in the milk of naturally infected sheep. In order to accomplish this, 275 lactating ewes were used; these were bred extensively in 17 estates distributed across nine cities. Polymerase chain reaction amplification was used to detect T. gondii DNA in milk samples, and the indirect ... more

deSantana Rocha; R.Lde Sousa Moura; B.M. Maciel; L.A. Guimarães; H.N.S. O’dwyer; A.D. Munhoz; G.R. Albuquerque
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Tail fat content affects meat quality, and it varies in different sheep breeds. Theoretically, lipid metabolism contributes to variation in tail fat content. Tail length, tail width, and tail girth were measured in live Tong sheep (with both short fat tail and long fat tail), Shaanbei fine wool sheep (long thin tail), Tan sheep (short fat tail), Kazakh sheep (hip fat tail), and Tibetan sheep ( ... more

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Liver tissue; RNA-seq; Sheep; Transcriptome

Research on gene regulation has been made possible with the help of RNA sequencing applications such as RNA-Seq technology for high-throughput sequencing platforms. Recent studies have explored the transcriptomes from different tissues of domestic animals using RNA-Seq technology, but little research has been done to study the transcriptomes of breeds of sheep having different adipose ... more

X. Cheng; S.G. Zhao; Y. Yue; Z. Liu; H.W. Li; J.P. Wu; X. Cheng; S.G. Zhao; Y. Yue; Z. Liu; H.W. Li; J.P. Wu
Animals; Apoptosis; Cells, Cultured; Chorionic Gonadotropin; Dose-Response Relationship, Drug; Female; Gene Expression Regulation; Gonadotropins, Equine; Horses; In Vitro Oocyte Maturation Techniques; Oocytes; Receptors, FSH; Receptors, LH; Receptors, LHRH; Sheep

We assessed the effects of equine chorionic gonadotropin (eCG) on oocyte in vitro maturation (IVM), apoptosis, and follicle-stimulating hormone receptor (FSHR), luteinizing hormone receptor (LHR), and gonadotropin-releasing hormone receptor (GnRHR) expression and mRNA levels. Cumulus-oocyte complexes (COCs) were recovered from sheep ovaries and pooled in groups, before being cultured in IVM ... more

S.C. Wei; Z.D. Gong; H.W. Zhao; H.Q. Liang; L.J. Lai; Y.Y. Deng
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Fiber diameter is a useful indicator of wool traits and it is the main determinant of wool quality and value. A comparative study was conducted to analyze the abundance and expression of 13 candidate genes using expression profile microarray analysis and to identify novel molecular markers associated with wool traits to provide a molecular basis for improving wool quality in sheep. Genes ... more

Y.Z. Tian; T. Usman; K.C. Tian; J. Di; X.X. Huang; X.M. Xu; H. Tulafu; W.W. Wu; X.F. Fu; Y. Bai; M. Tuerxun; A. Maimaiti
Copy number of variations; Insertions / deletions; Ovis aries; Sheep; SNPs

We report the whole ram genome of Jordanian Awassi (Ovis aries), screened using the power of Illumina HiSeq sequencing technology, providing insights into the ram's genomic structure. Generated data will help in the assessment of naturally occurring genetic variation and population structure of this sheep breed using different structure variation markers. ... more

N. Haddad; H. Migdadi; R. Al-Atiyat; K. Jawasre; S. Awabdeh; W. Obeidat; R. AlOmari; M. Aldamra; H. Ababneh; M.J. Tabbaa; M. Brake; M. Farooq
Antimicrobial peptides; Cathelicidins (CATHL); Tianzhu white yak

Cathelicidins are a major family of antimicrobial peptides (AMPs), an important component of innate immune system, playing a critical role in host defense and disease resistance in virtually all living species. Polymorphism and functional studies on cathelicidin of Tianzhu white yak contribute to understanding the specific innate immune mechanism in animals living at high altitudes in ... more

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Antimicrobial peptides; Chromatography; Membrane permeabilization; Phytopathogenic fungi; Reactive oxygen species

Coffee, an agronomical crop of great economic importance, is also among the most commonly traded commodities in worldwide markets. Antimicrobial peptides, which play a role in plant defense, have been identified and isolated particularly from seeds. We isolated and immunolocalized Cc-LTP2, a new lipid transfer protein (LTP) from Coffea canephora ... more

G.C.V. Bard; U. Zottich; T.A.M. Souza; S.F.F. Ribeiro; G.B. Dias; S. Pireda; M. Da Cunha; R. Rodrigues; L.S. Pereira; O.L.T. Machado; A.O. Carvalho; V.M. Gomes; G.C.V. Bard; U. Zottich; T.A.M. Souza; S.F.F. Ribeiro; G.B. Dias; S. Pireda; M. Da Cunha; R. Rodrigues; L.S. Pereira; O.L.T. Machado; A.O. Carvalho; V.M. Gomes