Research Article

Related GMR Articles

Cross-species amplification; Expressed sequence tags; Single sequence repeats; Sinonovacula constricta

Next-generation sequencing provides large-scale sequencing data with relative ease and at a reasonable cost, making it possible to identify a large amount of SSR markers in a timely and cost-effective manner. On the basis of the transcriptome database of Sinonovacula constricta obtained by Illumina/Solexa pyrosequencing, 60 polymorphic SSR markers were developed and characterized ... more

Y.H. Dong; H.H. Yao; C.S. Sun; D.M. Lv; M.Q. Li; Z.H. Lin; Y.H. Dong; H.H. Yao; C.S. Sun; D.M. Lv; M.Q. Li; Z.H. Lin; Y.H. Dong; H.H. Yao; C.S. Sun; D.M. Lv; M.Q. Li; Z.H. Lin
2016 Dec 02
DNA, Plant; Evolution, Molecular; Expressed sequence tags; Genetic variation; Genome, Plant; Microsatellite Repeats; Phylogeny; Saccharum

We analyzed 80 plants of the sugarcane (Saccharum spp) variety 'RB867515' in order to investigate its diversity and genetic structure at the molecular level. Four simple sequence repeat (SSR) loci (UGSM51, SMC1237, SEGMS1069, and UGSM38) and five expressed sequence tag (EST)-SSR loci (ESTA68, ESTB92, ESTB145, ESTC66, and ESTC84) were used as molecular markers. The polymorphic loci rate was 66. ... more

G.B. Maranho; R.C. Maranho; R. Desordi; A.F. das Neves; C.A. Mangolin; M.F.P.S. Machado
2017 Mar 08
Alleles; DNA, Plant; Expressed sequence tags; Genetic variation; Heterozygote; Microsatellite Repeats; Polymorphism, Genetic; Reproduction; Saccharum

The reduction in sugarcane productivity in subsequent cutting stages may be related to a gradual decrease of the allele number and mean observed heterozygosity (H) in the sugarcane ratoon. This hypothesis was tested assessing the number of alleles and H values in 10 expressed sequence tag microsatellites (Est-SSR loci) of the sugarcane varieties RB72454 and RB867515 in different cutting stages ... more

R. Augusto; R.C. Maranho; C.A. Mangolin; J.C.Bespalho Filho; M.F.P.S. Machado
2017 Apr 13
Amplified Fragment Length Polymorphism Analysis; Base Pairing; DNA Primers; Expressed sequence tags; Polymorphism, Genetic; Ricinus

The objective of this article was to develop TRAP (target region amplification polymorphism) primers for castor bean, with the goal of making functional markers available for genetic studies about the species. To do this, oligonucleotides were designed based on ESTs, obtained from the NCBI (National Center for Biotechnology Information) databank, which code enzymes involved in metabolic routes ... more

K.S. Simões; S.A. Silva; E.L. Machado; H.S. Brasileiro
2017 May 04
Alleles; Animals; Cyprinidae; Expressed sequence tags; Gene Transfer, Horizontal; Heterozygote; Microsatellite Repeats; Polymorphism, Genetic

We used next-generation sequencing technology to characterize 19 genomic simple sequence repeat (SSR) markers and 11 expressed sequence tag (EST) SSR markers from Leuciscus leuciscus baicalensis, a small freshwater fish that is widely distributed in Xinjiang, China. Primers were used to test for polymorphisms in three L. leuciscus baicalensis populations in Xinjiang. There were 4-27 (average ... more

D.J. Lei; G. Zhao; P. Xie; Y. Li; H. Yuan; M. Zou; J.G. Niu; X.F. Ma
2017 May 10
Alleles; Animals; Expressed sequence tags; Genetic markers; Heterozygote; Microsatellite Repeats; Perciformes; Polymorphism, Genetic; Transcriptome

Expressed sequence tags (ETSs) are the sources of microsatellite development. In this study, we isolated and characterized microsatellite markers for Odontobutis potamophila by using Illumina RNA-sequencing. We sequenced a large number of ESTs and screened 200 potential microsatellites. Consequently, a total of 56 novel polymorphic microsatellite repeat markers were identified in thirty-two ... more

P.P. Wang; M. Ren; S.Q. Chen; S.W. Yin; C. Zhao; H.Y. Zhang; X.R. Li; Q.Q. Cao; G.Q. Zhou
2017 May 31
Azospirillum brasilense; Expressed sequence tags; Genotype; Glutamate-Ammonia Ligase; Nitrate reductase; Nitrogen; Plant Proteins; Zea mays

The relative quantitative real-time expression of two expressed sequence tags (ESTs) codifying for key enzymes in nitrogen metabolism in maize, nitrate reductase (ZmNR), and glutamine synthetase (ZmGln1-3) was performed for genotypes inoculated with Azospirillum brasilense. Two commercial single-cross hybrids (AG7098 and 2B707) and two experimental synthetic varieties (V2 and V4) were raised ... more

L. Pereira-Defilippi; E.M. Pereira; F.M. Silva; G.V. Moro
2017 Aug 17
Animals; Expressed sequence tags; Malaysia; Microsatellite Repeats; Palaemonidae; Polymorphism, Genetic; Selective breeding

The Malaysian giant prawn is among the most commonly cultured species of the genus Macrobrachium. Stocks of giant prawns from four rivers in Peninsular Malaysia have been used for aquaculture over the past 25 years, which has led to repeated harvesting, restocking, and transplantation between rivers. Consequently, a stock improvement program is now important to avoid the depletion of wild ... more

K.H. Atin; A. Christianus; N. Fatin; A.C. Lutas; M. Shabanimofrad; B. Subha