Research Article

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03/12/2014
Ag-NOR; C-band; Cytogenetics; FISH; Loricaria cataphracta; NOR patterns

A cytogenetic analysis of Loricaria cataphracta revealed a diploid number of 2n = 64 chromosomes, distributed as 12 metacentric + 8 submetacentric + 2 subtelocentric + 42 acrocentric, with a fundamental number of 86. Analysis of the nucleolus organizing region (NOR) using silver nitrate impregnation and fluorescence in situ hybridization (18S rDNA probe) techniques ... more

F.E. Porto; B.S. Gindri; M.M.R. Vieira; L.A. Borin; A.L.B. Portela-Castro; I.C. Martins-Santos
06/02/2009
Genetic variability; Internal transcribed spacer; Melipona quinquefasciata; nuclear ribosomal DNA; Stingless bee

Melipona quinquefasciata is a ground-nesting South American stingless bee whose geographic distribution was believed to comprise only the central and southern states of Brazil. We obtained partial sequences (about 500-570 bp) of first internal transcribed spacer (ITS1) nuclear ribosomal DNA from Melipona specimens putatively identified as M. quinquefasciata collected ... more

J.O.P. Pereira; B.M. Freitas; D.M.M. Jorge; D.C. Torres; C.E.A. Soares; T.B. Grangeiro
09/30/2007
Ag-NOR; B chromosomes; C banding; Chromomycin A3; DAPI

We made a cytogenetic study of the fish Rhamdia quelen collected from the Bodoquena Plateau, an isolated national park region in Mato Grosso do Sul State, Brazil. The diploid number was 2n = 58, with 36 metacentric + 16 submetacentric + 6 subtelocentric chromosomes. We found one to three B chromosomes, which were metacentric and submetacentric and of medium size, showing both intra- ... more

V.P.O. Moraes; S.S. Cereali; O. Froehlich; A.L. Dias
03/04/2008
chromosome; Cytogenetics; Euphorbia heterophylla; Nucleolus

Euphorbia heterophylla L. (Euphorbiaceae) is a herbaceous species of great economic importance due to its invasive potential and consequent damage to agriculture and pasture land. For the first time, we provide information on its chromosome number, morphology, and behavior of mitotic chromosomes. Seeds were germinated and submitted to four treatments to obtain metaphases: 0.5% ... more

J.R. Aarestrup; D. Karam; G.W. Fernandes
12/11/2013
chromosome; Klinefelter’s syndrome; Male infertility; Preimplantation genetic diagnosis; Prenatal diagnosis

The purpose of this study was to detect chromosomal aberrations and azoospermia factor (AZF) microdeletions in male patients with reproductive problems and to summarize related clinical features to provide reliable information for evaluating prenatal and preimplantation diagnoses. A large cohort of 5083 men with various phenotypes of male infertility was analyzed via G-banding karyotyping ... more

Q. Quan; T.J. Li; X.P. Ding; J. Wei; L.X. Li; L. Fu
07/15/2013
45S rDNA; AgNOR-banding; Anura; chromosome; Evolution

Chromosome numbers, morphology, and nucleolus organizer region (NOR) locations are useful cytological characters for taxonomic and evolutionary studies. In this study, we provide the first cytogenetic analysis of Phyllomedusa bahiana and Phasmahyla spectabilis, and report new cytogenetic data on variation in NOR numbers and positions in Phyllomedusa rohdei and ... more

A. Barth; V.A. Souza; M. Solé; M.A. Costa
03/11/2013
Aegilops biuncialis; C-banding; chromosome; Fluorescence in situ hybridization; Karyotype

Aegilops biuncialis can be hybridized with wheat (Triticum spp) and has been used for wheat breeding and genetic studies. The A. biuncialis karyotype (Ub Ub Mb Mb) was investigated based on three A. biuncialis accessions grown in China. Two pairs of SAT chromosomes were identified as 1Ub and 5Ub ... more

J. Wang; W. Zhang; H. Zhao; F.R. Li; Z.G. Wang; J. Ji; X.Q. Zhang; D.W. Wang; J.M. Li
12/12/2014
Cannabis sativa; chromosome; Genetic diversity; Inter-simple sequence repeat

Hemp (Cannabis sativa) is an important fiber crop, and native cultivars exist widely throughout China. In the present study, we analyzed the genetic diversity of 27 important Chinese native hemp cultivars, by using inter-simple sequence repeats (ISSR) and chromosome markers. We determined the following chromosome formulas: 2n = 20 = 14m + 6sm; 2n = 20 = 20m; 2 ... more

L.G. Zhang; Y. Chang; X.F. Zhang; F.Z. Guan; H.M. Yuan; Y. Yu; L.J. Zhao
11/25/2015
chromosome; FISH; Multigene families

In this study, fluorescence in situ hybridization (FISH) analysis was used to determine and compare the numbers and chromosomal locations of two multigene families (rDNA and histone H3) in four Neotropical species of gomphocerine grasshoppers. FISH using the 18S rDNA probe identified a single site on the S9 chromosome of Amblytropidia sp and Cauratettix borelli, ... more

L.C. Silva-Neto; A.C.S. Bernardino; V. Loreto; R.C. Moura
10/25/2005
Africanized honeybee; Apidae; Apini; Enzymes; Esterase lipase; Glucosidases; Meliponini; Stingless bee

Hydrolytic enzymes from hypopharyngeal gland extracts of newly emerged, nurse and foraging workers of two eusocial bees, Scaptotrigona postica, a native Brazilian stingless bee, and the Africanized honey bee (Apis mellifera) in Brazil, were compared. The hypopharyngeal gland is rich in enzymes in both species. Fifteen different enzymes were found in the extracts, ... more

R.A.C. Costa; C. da Cruz-Landim

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