Research Article

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09/28/2015
Alkaline phosphatase; Bone mesenchymal stem cells; Osteocalcin; Osteopontin; Pulsed electromagnetic field

We investigated the effects of pulsed electromagnetic fields (PEMFs) of 20 Hz/2 mT on the osteogenic and adipogenic differentiation of bone marrow stem cells (BMSCs). Sprague Dawley rat BMSCs were isolated and cultured in vitro. The BMSCs of the third passage were obtained and stimulated by PEMFs of 20 Hz/2 mT. The alkaline phosphatase (ALP) activity was measured according to the ... more

T. Lu; Y.X. Huang; C. Zhang; M.X. Chai; J. Zhang
08/14/2015
Cardiac function; Myocardial infarction; N-ethylmaleimide-sensitive factor; RNA interference

This study examined the effects of N-ethylmaleimide-sensitive factor (NSF) small interfering RNA (siRNA) on cardiac function following myocardial infarction (MI) in rats. Thirty-six adult Sprague Dawley rats were randomly divided into three equivalent groups. An acute MI model was established by ligating the anterior descending branch of the left coronary artery and confirmed by ... more

Y. Zhou; Y. Liu; S.X. Yang; Z. Wang
12/16/2015
HER2/neu; Prostate cancer; RNA interference; Small-interfering RNA

The aim of this study was to investigate the effect of a small interfering RNA (siRNA) targeting human epidermal growth factor receptor 2 (HER2/neu) on the proliferation and viability of prostate cancer PC-3M cells. Chemically synthesized siRNA targeting HER2/neu was transfected into PC-3M cells by using liposomes, and cells transfected with empty liposomes, a negative siRNA sequence, or ... more

C.Y. Liu; P.C. Xu; D.G. Chen; X.H. Fan; M.Q. Li; X. Yang; Y.P. Xu
12/02/2015
CapG; Cell growth; Invasion; Metastasis; Prostate cancer; RNA interference

This study investigated CapG gene expression in prostate cancer cell lines; in addition, we explored the effects of CapG suppression on DU145 cell growth, and the underlying mechanism with which CapG affects DU145 cell growth and invasiveness. The expression of CapG and 18 related genes in DU145 cells was analyzed by flow cytometry, quantitative polymerase chain reaction (qPCR), ... more

B.K. Li; K. Guo; C.Y. Li; H.L. Li; P.P. Zhao; K. Chen; C.X. Liu
04/04/2016
Livin; Nanoparticle; PC-3M; Prostate cancer; RNA interference; Survivin

Monomethoxypolyethylene glycol-chitosan (mPEG-CS) nanoparticles were used as interfering RNA carriers to transfect human prostate cancer PC-3M cells to evaluate the effects of livin and survivin gene silencing on the proliferation and apoptosis. mPEG-CS nanoparticles with sizes of approximately 60 nm were first synthesized by ionic crosslinking. Through electrostatic adsorption, mPEG-CS- ... more

A.Q. Yang; P.J. Wang; T. Huang; W.L. Zhou; J. Landman; A.Q. Yang; P.J. Wang; T. Huang; W.L. Zhou; J. Landman; A.Q. Yang; P.J. Wang; T. Huang; W.L. Zhou; J. Landman
1/23/2017
Apoptosis; Cell Line, Tumor; Cell proliferation; Female; Gene Expression Regulation, Neoplastic; Humans; MicroRNAs; Proto-Oncogene Proteins c-bcl-2; RNA interference; Uterine cervical neoplasms

Cervical carcinoma is a life-threatening illness posing considerable danger to women's health. microRNAs (miRNAs) have been shown to regulate multiple cellular events, including growth and proliferation, and miR-187 is thought to regulate the growth and apoptosis of certain cell types. Our study focused on the influence of miR-187 on the growth, proliferation, and apoptosis of SiHa cervical ... more

C.Y. He; J. Yang
10/05/2017
Bmice-2; ilkworm; RNA interference; RNAI vector
Investigate the function of BmICE-2 gene in silkworm cells by constructing a BmICE-2 interference vector and analysing its interference effect. Online RNAi design software was used to predict the interference target sequence of Bmice-2. An optimized sequence was selected and inserted into the backbone of interference microRNA. The backbone of interference vector ... more
H. Yi; X. Ma
12/21/2015
Alpha-fetoprotein; Hepatitis C virus; Hepatocellular carcinoma; Polymorphism; Serum level; VEGF

We evaluated the influence of the vascular endothelial growth factor (VEGF) -C936T polymorphism on prognosis of hepatocellular carcinoma (HCC), cirrhosis, and hepatitis C virus (HCV) infection. Serum VEGF and alpha-fetoprotein (AFP) levels were determined and used to characterize sensitivity and specificity. A total of 285 subjects were studied: 68 HCC, 118 cirrhosis, 43 HCV, and 56 healthy ... more

E.Y. Yvamoto; R.F. Ferreira; V. Nogueira; M.A.S. Pinhel; G.D. Tenani; J.G.S.C. Andrade; M.E.L. Baitello; M.L. Gregório; P.S. Fucuta; R.F. Silva; D.R.S. Souza; R.C.M.A. Silva
12/07/2015
A1298C polymorphism; Hepatocellular carcinoma; Methylenetetrahydrofolate reductase (MTHFR)

The A1298C polymorphism of the methylenetetrahydrofolate reductase (MTHFR) gene has been reported to be associated with hepatocellular carcinoma (HCC), but there are conflicting results from previous studies. The present study aimed to investigate the association between this polymorphism and the risk of HCC using a meta-analysis of the published studies. Published literature ... more

W. Chang; Q. Meng; J.H. Liu; L.X. Wu; Y. Chen; S.D. Chen
12/07/2015
Hepatocellular carcinoma; Meta-analysis; T241M polymorphism; XRCC3

Numerous studies have evaluated the association between the X-ray repair complementing defective repair in Chinese hamster cells 3 (XRCC3) T241M polymorphism and hepatocellular carcinoma (HCC) risk. However, the results of such investigations have proved inconsistent. Therefore, we performed a meta-analysis of the association between this polymorphism and HCC risk in the Chinese ... more

R.B. Ji; Y.S. Qian; A.R. Hu; Y.R. Hu

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