Research Article

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08/07/2015
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There is limited information about microRNAs (miR­NAs) in H9N2 subtype influenza virus-infected chicken cells or tissues. In this study, 10,487,469 and 13,119,795 reads were obtained from in­fected and non-infected chicken embryo fibroblasts, respectively. Seven hundred and thirty-six and 1004 miRNAs, including mature miRNAs and precursors, were obtained from the infected and non-infected ... more

X. Peng; Q.S. Gao; L. Zhou; Z.H. Chen; S. Lu; H.J. Huang; C.Y. Zhan; M. Xiang
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Giant panda; Lactation; Mammary gland; miRNA

The giant panda (Ailuropoda melanoleuca) is one of the world’s most endangered mammals, and it has evolved several unusual biological and behavioral traits. During puberty, pregnancy, lactation, and involution, the mammary gland undergoes profound morphological and functional changes. A large number of microRNAs (miRNAs) have been identified to be involved in mammary gland ... more

C.D. Wang; K. Long; L. Jin; S. Huang; D.H. Li; X.P. Ma; M. Wei; Y. Gu; J.D. Ma; H. Zhang
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Breast cancer; ILIA; Insertion/deletion; miRNA; Polymorphism

The insertion/deletion polymorphism (rs3783553 TTCA/-) in the 3' untranslated region of interleukin-1A (IL1A) has been studied intensively and has been shown to affect tumor risk. We studied the frequency of the IL1A gene polymorphism rs3783553 and evaluated its relationship with breast cancer (BC). A hospital-based case-control study comprising 228 patients with histologically ... more

X. Huang; Y. Yang; Z.W. Cui; J. Wang; L.B. Gao; X. Huang; Y. Yang; Z.W. Cui; J. Wang; L.B. Gao
10/05/2018
DNA; miRNA; mRNA; Next-generation sequencing; Phalaenopsis; PhalDB

The orchid family Orchidaceae is one of the largest families of angiosperms; it includes approximately 35,000 species, displays a wide range of unique flower shapes and is a valued ornamental crop. Among the various species of orchids, the Phalaenopsis genus is the most widely commercialized among blooming potted plants. Our aim was to provide in-depth ... more

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Genetic analysis; Male sterility; Medicago truncatula; Tapetum

Heterosis, or enhancement through outbreeding, is one of the most promising approaches for increasing crop yield. Male sterility (MS), which promotes heterosis, has been widely applied in hybrid crop production. Medicago truncatula is a model legume species and is closely related to M. sativa, an important legume forage plant. Although the molecular mechanisms of MS in ... more

L.H. Shao; X.W. Zheng; D.X. Yi; C. Li; L.H. Shao; X.W. Zheng; D.X. Yi; C. Li
09/22/2015
Betula kirghisorum; Expression pattern; GRAS transcription factor; NaHCO3 stress; Subcellular localization

GRAS proteins are plant-specific transcription factors that are involved in the regulation of root and shoot growth. Here, we cloned BkGRAS2 from Betula kirghisorum (abbreviated to Bk) and analyzed the physicochemical properties and expression pattern of the encoded protein. BkGRAS2 had an open reading frame of 1614 bp encoding 537 amino acid residues. The deduced ... more

G.Y. Li; C.J. Yang; G.J. Liu
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CsICE1; Expression pattern; Real-time quantitative PCR; Tea plant

CsICE1 is thought to be involved in hardiness resistance of tea plants. Using seedling cuttings of biennial Wuniuzao in this study, the pattern of CsICE1 expression under cold temperature (4°, -5°C), drought [20% polyethylene glycol 6000 (PEG-6000)], and plant hormone [200 mg/L abscisic acid (ABA), 1 mg/L brassinolide (BR)] treatment was studied by real-time quantitative PCR. ... more

Z.T. Ding; C. Li; H. Shi; H. Wang; Y. Wang
09/22/2015
Bioinformatic analysis; Expression pattern; Fibroblast growth factor 5; Inner Mongolia Cashmere goat

Fibroblast growth factor 5 (FGF5) is a secreted signaling protein that belongs to the FGF family, and was found to be associated with hair growth in humans and other animals. The Inner Mongolia Cashmere goat (Capra hircus) is a goat breed that provides superior cashmere; this breed was formed by spontaneous mutation in China. Here, we report the cloning, molecular characterization, ... more

W.L. Bao; R.Y. Yao; Q. He; Z.X. Guo; C. Bao; Y.F. Wang; Z.G. Wang
07/06/2015
Chemoreception; Expression pattern; Rhynchophorus ferrugineus; Transcriptome

Olfaction is crucial for insects’ survival because it enables them to recognize various environmental information. It is primarily mediated by a large family of chemoreceptors, including olfactory receptors (ORs), gustatory receptors (GRs), and ionotropic receptors (IRs). Here, we assembled the transcriptome of the economically important pest of palms, Rhynchophorus ferrugineus, to ... more

W. Yan; L. Liu; W.Q. Qin; C.X. Li; Z.Q. Peng
11/23/2015
Banna mini-pig inbred line; Expression pattern; Gene structure; Phylogenetic analysis; Splice isoform; Testis-specific serine kinase

Testis-specific serine kinases (TSSKs) are a family of serine/threonine kinases highly expressed in the testes that are responsible for regulating many spermatogenesis-related protein activities. Mutations in this family have a positive relationship with oligospermia and azoospermia in human and mouse. Here, five members of the TSSK family from a Banna mini-pig inbred line (BMI) were cloned, ... more

P. Wang; H.L. Huo; S.Y. Wang; Y.W. Miao; Y.Y. Zhang; Q.L. Zhang; F.Q. Li; L.X. Liu; W.Z. Li; Y.Z. Zeng; J.L. Huo; H. Xiao

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