Research Article

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07/14/2015
Epigenetics; High-resolution melting; Imprinted genes; Methylation; Polymerase chain reaction

High-resolution melting (HRM) is considered an inexpensive, rapid, and attractive methodology for methylation analysis. In the application of the polymerase chain reaction (PCR) to methylation analysis, amplification efficiencies are biased towards unmethylated, rather than methylated, templates: a phenomenon known as PCR bias. To overcome PCR bias, primers that include CpG site(s) and ... more

F.V.M. Rubatino; N.V. Carobin; M.L. Freitas; V.T. de Oliveira; R.X. Pietra; P.P.R. Oliveira; A.A. Bosco; F.S. Jehee
08/19/2016
Behavior; Environmental enrichment; Epigenetics; Neurodevelopment; Post-traumatic stress disorder

Maternal post-traumatic stress disorder (PTSD) increases the risk of adverse neurodevelopmental outcomes in the child. Epigenetic alternations may play an essential role in the negative effects of PTSD. This study was aimed to investigate the possible epigenetic alterations of maternal PTSD, which underpins the developmental and behavioral impact. 24 pregnant Sprague-Dawley (SD) rats ... more

X.G. Zhang; H. Zhang; X.L. Liang; Q. Liu; H.Y. Wang; B. Cao; J. Cao; S. Liu; Y.J. Long; W.Y. Xie; D.Z. Peng; X.G. Zhang; H. Zhang; X.L. Liang; Q. Liu; H.Y. Wang; B. Cao; J. Cao; S. Liu; Y.J. Long; W.Y. Xie; D.Z. Peng
10/14/2017
Biomarkers; Colorectal cancer; DKK2 gene; DNA methylation; Epigenetics
Purpose: Epigenetic changes can be detected in precancerous lesions, suggesting that may be involved in the early stages of carcinogenesis. The methylation of specifics genes has been correlated with the outcome of many different types of cancers. This study compared the levels of DNA methylation between normal and tumor tissues from patients with colorectal ... more
T.D. Silva; A.V. Felipe; V.M. Vidigal; S.S. Saad; N.M. Forones
01/31/2022
Development; Epigenetics; Evolution of polyploid anurans; Transgenerational inheritance

This review deals with innovative concepts of evolution in vertebrates, such as epigenetic mechanisms and transgenerational inheritance. Evolutionary models based on data of fossil records, cytogenetics and molecular genetics are indicated. The 2R-model of vertebrate evolution is focalized as well as the epigenetic mechanisms of gene regulation and variability of ... more

M.L. Beçak
02/22/2016
1p36 deletion syndrome; Deletion size; Monosomy 1p36 syndrome; Phenotype

The major clinical features of monosomy 1p36 deletion are developmental delay and hypotonia associated with short stature and craniofacial dysmorphisms. The objective of this study was to review the cases of 1p36 deletion that was reported between 1999 and 2014, in order to identify a possible correlation between the size of the 1p36-deleted segment and the clinical phenotype of the ... more

C.F. Rocha; R.B. Vasques; S.R. Santos; C.L.A. Paiva; C.F. Rocha; R.B. Vasques; S.R. Santos; C.L.A. Paiva
12/02/2016
Animal Shells; Animals; Bivalvia; Breeding; Evolution, Molecular; Female; Fresh Water; Genetic variation; Genetics, Population; male; Microsatellite Repeats; Phenotype; Phylogeny; Quantitative trait loci

Four Hyriopsis cumingii populations, a breeding population (BP), a cultured population (FP), two wild populations from Poyang Lake (PY) and Dongting Lake (DT), and an H. schlegelii population were collected (JX), and the first filial generations (F1) were bred synchronously. The shell nacre polymorphisms, population genetic diversity, and genetic structures of the F1 of each population were ... more

L.M. Wu; Z.Y. Bai; X.J. Liu; C. Jin; H. Yin; J.L. Li
12/02/2016
Brassica rapa; Chromosome Mapping; Crosses, Genetic; DNA, Plant; Frameshift mutation; Genetic markers; Haploidy; Phenotype; Sequence Analysis, DNA; Transcription factors; Trichomes

Trichomes are derived from the epidermis and constitute an ideal system for studying cell division in plants. Here, a Chinese cabbage doubled haploid (DH) line (FT) without trichomes was crossed with another DH line (PurDH-1) with trichomes to develop an F population for fine mapping of trichome control genes. Genetic analysis showed that the trichome phenotype was controlled by a single ... more

X.L. Ye; F.Y. Hu; J. Ren; S.N. Huang; W.J. Liu; H. Feng; Z.Y. Liu
12/02/2016
Animals; Body weight; Cattle; Genome-wide association study; Genotype; Models, Biological; Phenotype; Polymorphism, Single Nucleotide; Quantitative trait loci

This study aimed to investigate the predictability of a phenotype when using a dynamic model of cattle growth. Genotypic and phenotypic information on Nellore (Bos indicus) cattle were used in a genome-wide association analysis designed to contrast the biological interpretation of core parameters [conversion efficiency of metabolizable energy to net energy for gain (k) and adjusted final ... more

M.C. Freua; M.H.A. Santana; R.V. Ventura; J.B.S. Ferraz
12/02/2016
Animals; Avian Proteins; Body weight; Breeding; Chickens; Female; Gene expression; Gene Expression Regulation; male; Muscle development; Myostatin; Organ Specificity; Phenotype; Quantitative trait loci

Myostatin (MSTN) is expressed in the myotome and developing skeletal muscles, and acts to regulate the number of muscle fibers. Wuding chicken large body, developed muscle, high disease resistance, and tender, delicious meat, and are not selected for fast growth. Broiler chickens (Avian broiler) are selected for fast growth and have a large body size and high muscle mass. Here, 240 one-day-old ... more

L.X. Liu; T.F. Dou; Q.H. Li; H. Rong; H.Q. Tong; Z.Q. Xu; Y. Huang; D.H. Gu; X.B. Chen; C.R. Ge; J.J. Jia
12/02/2016
Animals; Avian Proteins; Chickens; Female; Genetic association studies; Genotype; Insulin-like growth factor I; Phenotype; Polymorphism, Single-Stranded Conformational; Quantitative trait loci; Reproduction; Selection, Genetic

The insulin-like growth factor, IGF-I, plays an important role in the development of growth and reproductive traits. Single-strand conformation polymorphism analysis was used to detect and analyze polymorphisms and expression profiles of the IGF-I gene and its association with growth and reproductive traits of Jinghai yellow chickens. A point mutation g.295T>C was detected in the IGF-I gene ... more

M.A. Abdalhag; T. Li; L. Duan; T. Zhang; G. Zhang; J. Wang; Y. Wang

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