Research Article

Related GMR Articles

Calpastatin; Cyprinus carpio; Real-time PCR; Tissue expression

Calpastatin, an important protein used to regulate meat quality traits in animals, is encoded by the CAST gene. The aim of the present study was to clone the cDNA sequence of the CAST gene and detect the expression of CAST in the tissues of Cyprinus carpio. The cDNA of the C. carpio CAST gene, amplified using rapid amplification of cDNA ends ... more

W.X. Chen; Y. Ma
Coronavirus; COVID-19; D614G; Mutation; Population genetics; SARS-CoV-2

The COVID-19 pandemic is caused by the worldwide spread of the RNA virus SARS-CoV-2. Because of its mutational rate, wide geographical distribution, and host response variance this coronavirus is currently evolving into an array of strains with increasing genetic diversity. Most variants apparently have neutral effects for disease spread and symptom severity. ... more

F. Antoneli; T.N. Furuyama; I.M.V.G. Carvalho; M.R.S. Briones; L.M.R. Janini
Amplicon sequencing; AmpliSeq; Coronavirus; COVID-19; High-throughput sequencing; Viral genome

The SARS-CoV-2 pandemic has demonstrated the need for genomic epidemiology surveillance. To date, various methodologies have been applied, including metagenomic approaches and amplicon-based sequencing associated with high-throughput sequencing platforms. We adapted some details in amplicon-based sequencing using a SARS-CoV-2 community panel (Illumina AmpliSeq), ... more

C.P. Targueta; R.S. Braga-Ferreira; A.A. de Melo; J.S. de Curcio; R. Nunes; R.O. Dias; F. Mello-Andrade; D.M. Silva; E.P. Silveira-Lacerda; T.G. Castro; T.M.A. Pedroso; L.A. Pereira; A.F. Mendonça; R.M. Almeida; V.L. Silva; M.P.C. Telles
Chicken telomerase reverse transcriptase; Real-time PCR; Small-hairpin RNA

Here, we investigated the effects of blocking chicken telomerase reverse transcriptase (chTERT) in MDCC-MSB1 cells, using small-hairpin RNAs (shRNAs) to interfere with gene expression. shRNAs specific to chTERT mRNA were designed, cloned into DNA plasmid vectors, and transfected into MDCC-MSB1 cells. The transfected chTERT RNAs were expressed by the RNA polymerase ... more

N. Jiang; X.B. Zheng; Z.Y. Zhao; Z.G. Qin; T.J. Liu; N. Jiang; X.B. Zheng; Z.Y. Zhao; Z.G. Qin; T.J. Liu; N. Jiang; X.B. Zheng; Z.Y. Zhao; Z.G. Qin; T.J. Liu
GM crops; Real-time PCR; Transgenic maize

Conventional and genetically modified (GM) maize cultivars have been widely planted in Brazil to produce grains for processed food, feed, or to be consumed fresh as corn ears. This study used real-time PCR to detect GM maize in processed products and fresh commercial corn ears produced in the last two years in South Brazil. Eighteen conventional and GM maize ... more

C.A.M. Oliveira; C.M. Kommers; F.K.M. Lehmann; A.S.K. Fonseca; N. Ikuta; V.R. Lunge
Diagnosis; Enzyme-linked immunospot; Meta-analysis; Tuberculous pleurisy

Current methods for diagnosing tuberculous pleurisy are poor. Some studies have explored the diagnostic value of a pleural effusion enzyme-linked immunospot (ELISPOT) assay, but its accuracy remains controversial. Therefore, we performed a meta-analysis of the existing evidence on the ability of the ELISPOT assay to diagnose tuberculous pleurisy. We systematically searched PubMed, Google ... more

Z.Z. Li; W.Z. Qin; L. Li; Q. Wu; Y.J. Wang
Chronic obstructive pulmonary disease; Gene polymorphism; Meta-analysis; Vitamin D-binding protein

Gene polymorphism of vitamin D-binding protein (VDBP) correlates with chronic obstructive pulmonary disease (COPD), but the results remain inconclusive. We aimed to explore the association between VDBP gene polymorphism and COPD. We searched MEDLINE, Embase, Web of Science, and China National Knowledge Infrastructure for publications addressing the association between VDBP gene ... more

Y.L. Wang; H. Kong; W.P. Xie; H. Wang
COL1A1; Gene polymorphism; Meta-analysis; Osteoporosis

Studies investigating the association between the COL1A1 gene -1997G/T polymorphism and the risk of osteoporosis in postmenopausal women have reported conflicting results. We performed a meta-analysis based on the evidence currently available from the literature to make a more precise estimation of this relationship. We conducted searches of the published literature in the ... more

K.H. Yu; J. Tang; C.Q. Dai; Y. Yu; J.J. Hong
Cervical carcinoma; Gene polymorphism; Meta-analysis; XRCC1

Numerous studies have evaluated the association between Arg399Gln polymorphism of DNA repair gene XRCC1 and cervical carcinoma risk. However, the specific association is still controversial. To assess the relationship between XRCC1 Arg399Gln polymorphism and cervical carcinoma, we conducted a comprehensive meta-analysis of 10 case-control studies with 2051 cervical carcinoma cases and ... more

D.Y. Liu; H.C. Liang; X.M. Xiao
Interleukin 21; Meta-analysis; Polymorphism; Systemic lupus erythematosus

Several case-control studies have been conducted to investigate the association between Interleukin-21 (IL-21) polymorphisms and systemic lupus erythematosus (SLE) susceptibility, and most of the studies focused on IL-21 rs907715 and rs2221903 polymorphisms. Given the inconsistent results from these studies, the present meta-analysis aimed to obtain a more precise estimate of the ... more

J.H. Qi; J. Qi; L.N. Xiang; G. Nie