Research Article

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In the present study, we report on the case of a 43-year-old male patient seeking for fertility assistance, who showed a seminal analysis and testicular biopsy of complete azoospermia. Peripheral blood culture for chromosome studies revealed a karyotype of 46 chromosomes with a ring-Y-chromosome that lost the long arm heterochromatin. Molecular analysis of genomic DNA from the patient detected ... more

F.M. Carvalho; E.V. Wolfgramm; I. Degasperi; B.M. Verbeno; B.A. Vianna; F.F. Chagas; F. Paula; I.D. Louro; A.M.S. Perroni
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Germplasm characterization; Molecular markers; Passion flower; Principal coordinate analysis

Inter simple sequence repeat (ISSR) molecular markers were developed and used to investigate interspecific genetic variation in 25 wild species of Passiflora preserved in an active germplasm bank (BAG-Passifloras); intraspecific diversity was also analyzed in P. cincinnata accessions. Of 31 primers tested, 20 identified polymorphic loci with a total of 331 bands, ... more

A.G.R. Sousa; M.M. Souza; C.A.F. Melo; G.A. Sodré
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Incidence; Lineage group; Molecular markers; Rice; Sheath rot disease

To examine the impact of regional and seasonal variations on the incidence and severity of sheath rot, a major seed-borne disease of rice caused by Sarocladium oryzae, data on incidence and severity were collected from 27 selected fields in the Gazipur, Rangpur, Bogra, Chittagong, Comilla, Gopalgonj, Jessore, Manikgonj, and Bhola districts of Bangladesh in rain-fed and irrigated ... more

T.Islam Chowdhury; S. Mian; M.A.Taher Mia; M.Y. Rafii; M.A. Latif
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Apocynaceae; Genetic resources; Mangabeira; Molecular markers

Mangabeira (Hancornia speciosa Gomes) is a fruit tree of the Apocynaceae family, which is native to Brazil and is a very important food resource for human populations in its areas of occurrence. Mangabeira fruit is collected as an extractive activity, and no domesticated varieties or breeding programs exist. Due to a reduction in the area of ecosystems where it occurs, ... more

H.J. Jimenez; L.S.S. Martins; A.V.V. Montarroyos; J.F.Silva Junior; A.L. Alzate-Marin; R.M.Moraes Filho
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Molecular markers; Saccharum officinarum; Somaclonal variants

Sugarcane culture is an important source of income for the Brazilian economy. The aim of this study was to identify somaclonal variation in sugarcane varieties RB943365 and RB92579 arising from micropropagation using inter-simple sequence repeat (ISSR) DNA markers. The evaluated plants were generated from the in vitro propagation of shoot tips grown in MS medium supplemented with ... more

B.S. Hsie; J.Z. Brito; M.X.Vila Nova; L.R. Borges-Paluch; M.V. Silva; V.M.S.T. Donato
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FATP1 gene; Meat quality traits; Molecular markers; Qinchuan cattle; Tissue expression

Fatty acid transport protein 1 (FATP1), an integral membrane protein that facilitates long-chain fatty acid influx, is involved in the genetic network for oleic acid synthesis. The aim of this study was to examine the association of FATP1 polymorphisms with live animal meat quality traits in Chinese Qinchuan cattle. Quantitative real-time PCR analysis demonstrated that FATP1 ... more

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In vitro; Micropropagation; Molecular markers; Regeneration; Shoot tips; Somaclonal variation

Crambe (Crambe abyssinica) is a non-edible annual herb, which was first cultivated to extract oil for industry, and now has great potential for biodiesel production. The objective of this investigation was to evaluate the genetic stability of micropropagated plants of the C. abyssinica Hochst cultivar ‘FMS brilhante’ using polymerase chain reaction techniques based on ... more

E.T. Werner; T.C.B. Soares; A.B.P.L. Gontijo; J.D.Souza Neto; J.A.Tdo Amaral
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Effect evaluation; Grain gene; Molecular markers; Rice; Verification

Grain size is an important trait that directly influences the yield of rice. Validation and evaluation of grain genes is important in rice genetic studies and for breeding. In a population of 240 recombinant inbred lines (RILs) derived from a cross between an extra-large grain japonica variety TD70 and a small grain indica variety Kasalath, we mapped 19 QTLs controlling grain ... more

Y.D. Zhang; J. Zheng; Z.K. Liang; Y.L. Liang; Z.H. Peng; C.L. Wang
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Glycine max; Molecular markers; NIR; SSR

The present study was undertaken to detect and map the quantitative trait loci (QTL) related to soybean oil content. We used 244 progenies derived from a bi-parental cross of the Lineage 69 (from Universidade Estadual Paulista “Júlio de Mesquita Filho”/Faculdade de Ciências Agrárias e Veterinárias - Breeding Program) and Tucunaré cultivar. A total of 358 simple sequence repeat (SSR; ... more

D.C. Leite; J.B. Pinheiro; J.B. Campos; A.O. Di Mauro; S.H. Unêda-Trevisoli; D.C. Leite; J.B. Pinheiro; J.B. Campos; A.O. Di Mauro; S.H. Unêda-Trevisoli; D.C. Leite; J.B. Pinheiro; J.B. Campos; A.O. Di Mauro; S.H. Unêda-Trevisoli
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DarT_seq; Early selection; Linkage disequilibrium; Molecular markers; Selective accuracy

Genome wide selection (GWS) is essential for the genetic improvement of perennial species such as Citrus because of its ability to increase gain per unit time and to enable the efficient selection of characteristics with low heritability. This study assessed GWS efficiency in a population of Citrus and compared it with selection based on ... more

I.B. Gois; A. Borém; M. Cristofani-Yaly; M.D.V. de Resende; C.F. Azevedo; M. Bastianel; V.M. Novelli; M.A. Machado; I.B. Gois; A. Borém; M. Cristofani-Yaly; M.D.V. de Resende; C.F. Azevedo; M. Bastianel; V.M. Novelli; M.A. Machado; I.B. Gois; A. Borém; M. Cristofani-Yaly; M.D.V. de Resende; C.F. Azevedo; M. Bastianel; V.M. Novelli; M.A. Machado

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