Research Article

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08/03/2010
Cerrado; Erythrinidae; Microsatellite; Shotgun library

We identified 14 microsatellite loci for the wolf fish, Hoplias malabaricus (Erythrinidae), from a genomic shotgun library. Twenty-five primers were designed, and 48 individuals of H. malabaricus from four localities of northwest Goiás, in central Brazil, were genotyped to characterize the polymorphism at each locus. Fourteen primers amplified clearly interpretable ... more

S.G.C.A. Gondim; L.V. Resende; R.P.V. Brondani; R.G. Collevatti; N.J. Silva-Júnior; R.R. Pereira; M.P.C. Telles
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Alouatta caraya; Assignment test; Brazil; Cerrado; Pedigree; Philopatry

We here investigated the kin structure and pattern of dispersal in the black-and-gold howler monkey (Alouatta caraya, Platyrrhini, Atelidae) based on genotype differences at nine microsatellite loci of 48 individuals from eight social groups along the riparian forest of the Tocantins River, Brazil. The genetic diversity (HE = 0.647) was similar to or higher ... more

R.G. Collevatti; A.C. Souza-Neto; N.J. Silva-Jr.; M.P.C. Telles
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Cerrado; Conservation; Genetic variability; Germplasm; Multivariate techniques

The fragmentation of the original vegetation of the Cerrado biome, caused by the expansion of agricultural areas, mainly in central-west Brazil, calls for an assessment of the native population of this vegetation, especially of the species of interest for domestication and sustainable use. The purpose of this study was to characterize the genetic diversity of 140 gabiroba mother plants ( ... more

E.S. de Assis; E.Fdos Reis; J.F.N. Pinto; L.A.S. Contim; L.A.S. Dias
08/21/2013
Cagaita; Cerrado; Genetic diversity; Microsatellite; Shotgun library

Microsatellite markers were developed for population genetic analyses of the Neotropical tree Eugenia dysenterica DC (Myrtaceae), after construction of a shotgun genomic library for microsatellite discovery. Nine primers were designed, of which 5 yielded amplified product. These primers were polymorphic for 97 individuals collected in 3 distinct localities. The number of alleles ... more

M.P.C. Telles; J.B. Silva; L.V. Resende; R.P. Vianello; L.J. Chaves; T.N. Soares; R.G. Collevatti
09/26/2012
Cerrado; Chiroptera; Conservation; Genetic diversity; Neotropical bat; Shotgun genomic library

Glossophaga soricina is a widespread Neotropical nectarivorous bat. We characterized 10 microsatellite loci isolated from a shotgun genomic library. We analyzed tissues from wing membrane of 67 individuals collected from two populations of Central Brazil (Brasília and Alto Paraíso). The number of alleles per locus ranged from 2 to 20, and the observed and expected ... more

M. Oprea; F.P. Peixoto; L.V. Resende; R.G. Collevatti; M.P.C. Telles
06/15/2012
Cerrado; Climatic change; Geographic range shift; Microsatellites

Many species are expected to suffer strong shifts in their geographic ranges due to climate changes in the next 50 years, with severe consequences for biodiversity patterns and population structure. We used here an ensemble forecast approach for obtaining species’ range in which multiple species distribution models and climatic models were combined to model loss of genetic variability in ... more

J.A.F. Diniz-Filho; R.G. Collevatti; L.J. Chaves; T.N. Soares; J.C. Nabout; T.F. Rangel; D.B. Melo; J.S. Lima; M.P.C. Telles
03/22/2012
Cerrado; Dimorphandra mollis; DNA extraction; High-quality DNA; Polysaccharide-free DNA; Secondary metabolites

Dimorphandra mollis (Leguminosae), known as faveiro and fava d’anta, is a tree that is widely distributed throughout the Brazilian Cerrado (a savanna-like biome). This species is economically valuable and has been extensively exploited because its fruits contain the flavonoid rutin, which is used to produce medications for human circulatory diseases. Knowledge ... more

H.A.V. Souza; L.A.C. Muller; R.L. Brandão; M.B. Lovato
06/11/2015
Babassu; Cerrado; Habitat fragmentation

Attalea vitrivir Zona (synonym Orbignya oleifera) is one of the six species of Arecaceae known as “babassu”. This species is used to make cosmetics, food, and detergents due to the high concentration of oil in the seeds. It is found only in fragmented areas of southern Bahia State and northern Minas Gerais State, southeast Brazil, and this fragmentation has affected ... more

R.R.M. Santos; M.M. Cavallari; M.A.S. Pimenta; A.G. Abreu; M.R. Costa; M.L. Guedes
02/26/2019
Brazilian fruit; Conservation; Germplasm; Hancornia speciosa; SSR; Variability

The mangaba, Hancornia speciosa, (Apocynaceae) is a fruit tree native to Brazil with predominantly extractivist production. The fruit can be consumed in natura; however, it is widely consumed as frozen pulp and ice cream produced by agro-industry companies. We evaluated the genetic diversity of 213 individuals that make up the mangaba GeneBank of ... more

A.V.C. Silva; M.F. Vitória; V.R.R. Azevedo; A.J. Sá; A.L.S. Nascimento; M.N. Cardoso; A.N.R. Soares; J.F.Silva Júnior1; A.S. Lédo
03/09/2007
Azoospermia; Infertility; Molecular markers; Ring-Y

In the present study, we report on the case of a 43-year-old male patient seeking for fertility assistance, who showed a seminal analysis and testicular biopsy of complete azoospermia. Peripheral blood culture for chromosome studies revealed a karyotype of 46 chromosomes with a ring-Y-chromosome that lost the long arm heterochromatin. Molecular analysis of genomic DNA from the patient detected ... more

F.M. Carvalho; E.V. Wolfgramm; I. Degasperi; B.M. Verbeno; B.A. Vianna; F.F. Chagas; F. Paula; I.D. Louro; A.M.S. Perroni

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