Research Article

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Herein, we describe 34 microsatellite loci developed using an enrichment genomic library for the tree species Hancornia speciosa Gomes (Apocynaceae). Thirty-five individuals were genotyped using 34 primers to analyze the polymorphisms at each locus. The number of alleles per locus ranged from 4 to 20. The average number of alleles was 8.11, and the expected heterozygosity ranged from ... more

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Cerrado; Computational search; Conservation planning; Genetic diversity; Optimization; R platform

Conservation strategies routinely use optimization methods to identify the smallest number of units required to represent a set of features that need to be conserved, including biomes, species, and populations. In this study, we provide R scripts to facilitate exhaustive search for solutions that represent all of the alleles in networks with the smallest possible number of populations. ... more

J.A.F. Diniz-Filho; J.V.B.P.L. Diniz; M.P.C. Telles; J.A.F. Diniz-Filho; J.V.B.P.L. Diniz; M.P.C. Telles; J.A.F. Diniz-Filho; J.V.B.P.L. Diniz; M.P.C. Telles
12/22/2015
Apocynaceae; Genetic resources; Mangabeira; Molecular markers

Mangabeira (Hancornia speciosa Gomes) is a fruit tree of the Apocynaceae family, which is native to Brazil and is a very important food resource for human populations in its areas of occurrence. Mangabeira fruit is collected as an extractive activity, and no domesticated varieties or breeding programs exist. Due to a reduction in the area of ecosystems where it occurs, ... more

H.J. Jimenez; L.S.S. Martins; A.V.V. Montarroyos; J.F.Silva Junior; A.L. Alzate-Marin; R.M.Moraes Filho
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Endangered species; Genetic variability; ISSR; Molecular markers; RAPD

The goal of this study was to characterize the structure of two natural populations of the coral tree using RAPD and ISSR markers. The study evaluated all individuals in two different areas in the northeastern region of Brazil: the first was in the riparian area, 10 km x 100 m along the edge of the lower São Francisco River, and the second was in the municipality of Pinhão, in a semiarid ... more

M.F.V. Melo; L.O. Gonçalves; A.R.C. Rabbani; S.V. Álvares-Carvalho; J.B. Pinheiro; M.I. Zucchi; R. Silva-Mann
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Anthropic effects; Endangered species; Genetic variability; Molecular markers; Population genetics

Phyla scaberrima (Verbenaceae) is a herbaceous peren­nial species that is distributed from Mexico (center of origin) to Colom­bia, growing in forest and swamp edges or grasslands from sea level up to an altitude of 1800 m. The chemical properties and uses in popular medicine have drastically affected the population size of this species. In this study, we investigated genetic ... more

L.G. Androcioli; E.A. Ruas; L.A. Rodrigues; C.F. Ruas; H.E.R. Perilla; P.M. Ruas
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Cerdocyon thous; Cerrado; Chrysocyon brachyurus; Cross-amplification; STR markers

The maned wolf (Chrysocyon brachyurus) and the crab-eating fox (Cerdocyon thous) are two wild-canid species found in the Brazilian Cerrado. We tested cross-amplification and transferability of 29 short tandem repeat primers originally developed for cattle and domestic dogs and cats on 38 individuals of each of these two species, collected in the Emas National Park, ... more

F.M. Rodrigues; M.P.C. Telles; L.V. Resende; T.N. Soares; J.A.F. Diniz-Filho; A.T.A. Jácomo; L. Silveira
11/02/2008
Allozymes; Asteraceae; Campo rupestre; Cerrado; Eremanthus glomerulatus; mtDNA

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A.G. Abreu; V.N. Solferini
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Cerrado; Pau-papel; Shotgun library; SSR; STR

We isolated and characterized 12 microsatellite loci for Tibouchina papyrus (Melastomataceae), an endangered species with narrow and disjunct range, endemics to a few localities in “cerrado rupestre” from Central Brazil. These microsatellites were obtained by sequencing of a genomic shotgun library for primer design. Leaves from 96 individuals collected in the three known local ... more

M.P.C. Telles; F.P. Peixoto; J.S. Lima; L.V. Resende; R.P. Vianello; M.E.M.T. Walter; R.G. Collevatti
03/01/2011
Cerrado; Dimorphandra mollis; DNA extraction; Metabolic compounds; “Fava d’anta”

Isolation of high-quality DNA from plants, especially plants from the Cerrado, is notoriously difficult because of polysaccharides and secondary compounds produced by plants from this biome. DNA isolation and its quality may be compromised by chemical defenses such as tannins and phenols. Quantitative plant defenses tend to have a cumulative effect, increasing in concentration during ... more

P.A. Moreira; D.A. Oliveira
09/08/2010
Cerrado; Loricariidae; Microsatellite; Shotgun library

We isolated and characterized 10 microsatellite loci in the armored catfish (Hypostomus gymnorhynchus, Loricariidae), using a genomic shotgun library to obtain the repetitive sequences. Twenty-four primers were designed and 14 individuals of H. gymnorhynchus from the Caiapó River, in central Brazil, were genotyped using these primers to analyze the polymorphism at each ... more

M.P.C. Telles; L.V. Resende; R.P.V. Brondani; R.G. Collevatti; M.C. Costa; N.J.Silva Júnior

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