Research Article

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5-Enopyruvylshikimate-3-phosphate synthase; Clone; Escherichia coli; Pantoea sp

The shikimate pathway enzyme 5-enopyruvylshikimate-3-phosphate synthase (EPSPS) is the target of the broad spectrum herbicide glyphosate. A novel aroA gene encoding an EPSPS from Pantoea sp was identified and subcloned into the pET-28a vector to construct the recombinant pET-AroAPantoea sp plasmid. Amino acid sequence analysis indicated that AroA ... more

F. Liu; Y.P. Cao
06/17/2016
4D8; Clone; Hyalomma asiaticum

Hyalomma asiaticum tick, an important ectozoic parasite causes tickle, pain, anemia, weight loss, and paralysis in its hosts, which include humans, cattle, sheep, horses, camels, and hares. The 4D8 gene can be a potential vaccine candidate antigen for H. asiaticum. In the present study, we cloned and expressed the 4D8 gene of H. asiaticum from Xinjiang Province ... more

Z.Q. Liu; J. Xia; G.L. Wang; N. Kuermanali; Z.Q. Liu; J. Xia; G.L. Wang; N. Kuermanali
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Beta vulgaris; Bioinformatics; EST; GSS; MicroRNA

Highly conserved endogenous non-coding microRNAs (miRNAs) play important roles in plants and animals by silencing genes via destruction or blocking of translation of homologous mRNA. Sugar beet, Beta vulgaris, is one of the most important sugar crops in China, with properties that include wide adaptability and strong tolerance to salinity and impoverished soils. Seedlings of ... more

J.L. Li; J. Cui; D.Y. Cheng
10/30/2015
Bacteria; Bioinformatics; Fungi; Laccase; Oomycetes; Plant pathogen

To date, research on laccases has mostly been focused on plant and fungal laccases and their current use in biotechnological applications. In contrast, little is known about laccases from plant pathogens, although recent rapid progress in whole genome sequencing of an increasing number of organisms has facilitated their identification and ascertainment of their origins. In this study, a ... more

B.Z. Feng; P.Q. Li; L. Fu; X.M. Yu
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Alveolar bone cell; Bioinformatics; Biological pathway; Diabetes

Alveolar bone osteoblasts are widely used in dental and related research. They are easily affected by systemic diseases such as diabetes. However, the mechanism of diabetes-induced alveolar bone absorption remains unclear. This study systematically explored the changes in human alveolar bone cell-related gene expression and biological pathways, which may facilitate the investigation of ... more

X. Sun; Q.H. Ren; L. Bai; Q. Feng
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Bioinformatics; Chordates; Evolutionary biology; Myogenic regulatory factors

MyoD and MyoG are transcription factors that have essential roles in myogenic lineage determination and muscle differentiation. The purpose of this study was to compare multiple amino acid sequences of myogenic regulatory proteins to infer evolutionary relationships among chordates. Protein sequences from Mus musculus (P10085 and P12979), human Homo sapiens (P15172 and ... more

L.C. Oliani; K.C.F. Lidani; J.E. Gabriel
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Bioinformatics; Identification; MicroRNA; Middleware; RNA sequencing; Web server

This report describes the miRQuest - a novel middleware available in a Web server that allows the end user to do the miRNA research in a user-friendly way. It is known that there are many prediction tools for microRNA (miRNA) identification that use different programming languages and methods to realize this task. It is difficult to understand each tool and apply it to diverse datasets ... more

R.R. Aguiar; L.A. Ambrosio; G. Sepúlveda-Hermosilla; V. Maracaja-Coutinho; A.R. Paschoal; R.R. Aguiar; L.A. Ambrosio; G. Sepúlveda-Hermosilla; V. Maracaja-Coutinho; A.R. Paschoal; R.R. Aguiar; L.A. Ambrosio; G. Sepúlveda-Hermosilla; V. Maracaja-Coutinho; A.R. Paschoal
06/18/2019
Bioinformatics; Conserved domains; Homology modeling

The Allium genus stands out for its uses in human food and also for its medicinal properties. Many representatives of the Amaryllidaceae family are known for producing mannose binding lectins (MBL). In plants, lectins act as reserves of proteins that can be used for plant growth and development and also in defense against herbivores and pathogens, being ... more

H.J. Jimenez; L.S.S. Martins; R. Carvalho; A.V.V. Montarroyos; R.M.Moraes Filho
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Alpha-galactosidase; Bioinformatics; Functional prediction; Hypothetical proteins; Penicillium rubens

Advances in biotechnology and molecular biology fields have allowed the sequencing of species genomes to improve the understanding of their genetics. As a consequence of the large number of whole genome sequencing projects, various DNA and protein sequences predicted by bioinformatics still without an identified function and are annotated in data banks as ... more

F.F. Silva; D.B. Gonçalves; D.O. Lopes
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Analysis workflow; Bioinformatics; Corpse identification; Forensic samples

The forensic community is concerned about the quality control of mitotypes reported, as probability estimates may be biased to the disadvantage of a suspect when there is incomplete data. This is particularly important when using new technology such as Massively Parallel Sequencing (MPS), which performs simultaneous analysis of millions of DNA fragments, generating ... more

C. Bottino; R. Silva; R.S. Moura-Neto