Research Article

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09/30/2007
Acrididae; Gene mapping; Hsp; In situ hybridization; Schistocerca pallens; Single-copy genes

There have been many studies on Schistocerca gregaria and Locusta migratoria, which are important grasshopper pests in many parts of the world. However, the main pest grasshopper species in Brazil, S. pallens, Rhammatocerus schistocercoides and Stiphra robusta, are very poorly characterized genetically. We adapted a permanent in situ ... more

T.T. Rieger; S.V. Oliveira-Silva; I.A. Pachêco; B.S. Chagas; J.F. Santos
11/29/2011
C-banding; In situ hybridization; Leaf rolling; Triticale; X Triticosecale Wittmack

Leaf rolling occurs in some cereal genotypes in response to drought. We identified and made a phenotypic, cytological and physiological analysis of a leaf-rolling genotype (CMH83) of hexaploid triticale (X Triticosecale Wittmack) that exhibited reduced plant height, rolled and narrow leaves. Gliadin electrophoresis of seed protein showed that CMH83 was genetically stable. ... more

E.N. Yang; Z.J. Yang; J.F. Zhang; Y.C. Zou; Z.L. Ren
10/03/2011
Broad-complex; Gene expression; In situ hybridization; RACE-PCR; Real-time PCR

Broad-Complex (BR-C) is an early ecdysone-responsive gene encoding a family of zinc-finger transcription factors that function during metamorphosis in insects. We identified two full-length cDNAs of BR-C Z6 in the giant tiger shrimp (Penaeus monodon). They were 2422 and 2060 bp in length, containing open reading frames of 1440 and 1443 bp, corresponding to ... more

A. Buaklin; S. Klinbunga; P. Mensveta
03/09/2011
Bacterial artificial chromosomes; Chromosome variation; In situ hybridization; Pepper

Carotenoids are responsible for a range of fruit colors in different hot pepper (Capsicum) varieties, from white to deep red. Color traits are genetically determined by three loci, Y, C1, and C2, which are associated with carotenogenic genes. Although such genes have been localized on genetic maps of Capsicum and anchored in Lycopersicon ... more

V. Andrade-Souza; M.G.C. Costa; C.X. Chen; F.G. Gmitter; M.A. Costa
03/09/2011
Gene expression; In situ hybridization; Planarian; Regeneration; STAG

We examined the spatial and temporal expression of the planarian Dugesia japonica STAG-related gene (DjStag), in both intact and regenerating planarians, by whole-mount in situ hybridization and relative quantitative real-time PCR. The first localized transcripts of DjStag were detected in the blastemas three days after amputation, in all regenerates including those ... more

Z.Q. Yuan; B.S. Zhao; J.Y. Zhang
12/02/2013
Expression analysis; Hyriopsis cumingii; In situ hybridization; Laminin receptor precursor; Shell repair

Hyriopsis cumingii is an economically important freshwater pearl mussel with high pearl quality that is endemic in China. Investigation of genes relevant to shell formation is important for increased pearl output. The substances that form mollusk shells are secreted by epithelial cells in the mantle, the proliferation of which influences secretion ability. This study focused on ... more

X.Z. Chang; J.L. Li; Z.Y. Bai; X.L. Li
01/23/2015
Cashmere goats; In situ hybridization; Real-time polymerase chain reaction; RORα; RORα expression

The expression of retinoid-acid-related orphan receptor α (RORα) was evaluated at the mRNA level using real-time polymerase chain reaction (qRT-PCR), and its expression localization was determined by in situ hybridization of adult Inner Mongolian cashmere goats at different times of the year. In situ hybridization demonstrated that RORα ... more

Y.H. Zhao; Z.H. Liu; L. Wang; H.M. Xiao; C.G. Du; Y.J. Zhang; R. Su; J.Q. Li
05/11/2007
Adrenomedullin; Leukocyte; RNA; RT-PCR

Blood samples are used as a biological source to discover biomarkers of hematological and non-hematological disorders. The present study shows the impact of different experimental conditions associated with cell lysis buffer, TRI-reagent protocol and blood cell storage buffer and their correlation with the quantity, quality and Adrenomedullin gene expression levels of total ... more

I.A.G. Pérez; S.P. Santana; T.D. Argudin; D.O.P. Gardon
12/08/2011
Cytokinin; Duplication; Maize (Zea mays); Phylogenetic analysis; RT-PCR; Two-component signal system

Cytokinins play many vital roles in plant development and physiology. In plants, cytokinin signals are sensed and transduced by the two-component signal system. This signaling cascade is typically composed of three proteins: a sensory histidine kinase, a histidine phosphotransfer protein, and a response regulator. Through a comprehensive genome-wide analysis of the maize (Zea mays ... more

Z.X. Chu; Q. Ma; Y.X. Lin; X.L. Tang; Y.Q. Zhou; S.W. Zhu; J. Fan; B.J. Cheng
11/22/2011
Cell wall; Polygalacturonase; RT-PCR; Sweet pepper; Transmission electron microscopy; Ultrastructure

Activity and expression of polygalacturonase (PG), a hydrolytic enzyme involved in ultrastructural changes in the pericarp of sweet pepper (Capsicum annaum), were investigated at different ripening stages of the pepper cultivars Mandi and Talanduo. Molecular cloning of CaPG was carried out by constructing a cDNA library from three stages of fruit ripening. Morphological ... more

S.S. Ahmed; Z.H. Gong; M.A. Khan; Y.X. Yin; W.L. Guo; J. Imran

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