Research Article

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CsICE1 is thought to be involved in hardiness resistance of tea plants. Using seedling cuttings of biennial Wuniuzao in this study, the pattern of CsICE1 expression under cold temperature (4°, -5°C), drought [20% polyethylene glycol 6000 (PEG-6000)], and plant hormone [200 mg/L abscisic acid (ABA), 1 mg/L brassinolide (BR)] treatment was studied by real-time quantitative PCR. ... more

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Bioinformatic analysis; Expression pattern; Fibroblast growth factor 5; Inner Mongolia Cashmere goat

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Banna mini-pig inbred line; Expression pattern; Gene structure; Phylogenetic analysis; Splice isoform; Testis-specific serine kinase

Testis-specific serine kinases (TSSKs) are a family of serine/threonine kinases highly expressed in the testes that are responsible for regulating many spermatogenesis-related protein activities. Mutations in this family have a positive relationship with oligospermia and azoospermia in human and mouse. Here, five members of the TSSK family from a Banna mini-pig inbred line (BMI) were cloned, ... more

P. Wang; H.L. Huo; S.Y. Wang; Y.W. Miao; Y.Y. Zhang; Q.L. Zhang; F.Q. Li; L.X. Liu; W.Z. Li; Y.Z. Zeng; J.L. Huo; H. Xiao
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MicroRNAs (miRNAs) are a class of non-coding endogenous negative regulators that regulate gene expression at both the transcriptional and post-transcriptional levels. However, little is known about the expression characteristics of miRNAs during pollen development in Brassica oleracea. In this study, five known and three novel miRNAs were identified and their expression patterns were ... more

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Crustacean; Expression pattern; Feminization-1 homolog b (Fem1b); Macrobrachium nipponense; Post-larval; RT-qPCR

Feminization-1 homolog b (Fem1b) is one of the genes essential for male development and play central roles in sex determination of Caenorhabditis elegans. In this study, we cloned and characterized the full-length Fem1b cDNA from the freshwater prawn Macrobrachium nipponense (MnFem1b) in different tissues and at different developmental stages. ... more

N.M.A. Rahman; H. Fu; H. Qiao; S. Jin; H. Bai; W. Zhang; F.W. Jiang; G. Liang; S. Sun; Y. Gong; F.F. Jiang; Y. Xiong; Y. Wu; N.M.A. Rahman; H. Fu; H. Qiao; S. Jin; H. Bai; W. Zhang; F.W. Jiang; G. Liang; S. Sun; Y. Gong; F.F. Jiang; Y. Xiong; Y. Wu
09/22/2015
Genetic diversity; Genic-SSRs; Nelumbo nucifera; Transcriptome

Nelumbo nucifera is an important economic vegetable and traditional medicine, but available genetic resources remain limited. Next generation sequencing has proven to be a rapid and effective means of identifying genic simple sequence repeat (genic-SSR) markers. This study developed genic-SSRs for N. nucifera using Illumina sequencing technology to assess diversity across ... more

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Heat shock protein; high temperature; Transcriptome; Tree peony leaf

The tree peony leaf is an important vegetative organ that is sensitive to abiotic stress and particularly to high temperature. This sensitivity affects plant growth and restricts tree peony distribution. However, the transcriptomic information currently available on the peony leaf in public databases is limited. In this study, we sequenced the transcriptomes of peony leaves subjected to ... more

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Assembly; Brontispa longissima; Transcriptome

The coconut leaf beetle, Brontispa longissima, is a de­structive pest of palm plants. Although its ecological and biological characteristics are well understood, its genetic information remains largely unknown. To advance our understanding of its molecular ba­sis of biology and ecology, we sequenced and analyzed its whole tran­scriptome by using high-throughput Illumina paired- ... more

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Eleocharis dulcis; High-throughput sequencing; Simple sequence repeat; Transcriptome

Water chestnut (Eleocharis dulcis) is an important aquatic crop in China; however, transcriptomic and genomic data in public databases are limited. To identify genes and development molecular markers, high-throughput transcriptome sequencing was applied to generate transcript sequences from water chestnut leaf. More than 24 million reads were obtained, trimmed, and assembled into ... more

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