Research Article

Related GMR Articles

Genetic variability; Internal transcribed spacer; Melipona quinquefasciata; nuclear ribosomal DNA; Stingless bee

Melipona quinquefasciata is a ground-nesting South American stingless bee whose geographic distribution was believed to comprise only the central and southern states of Brazil. We obtained partial sequences (about 500-570 bp) of first internal transcribed spacer (ITS1) nuclear ribosomal DNA from Melipona specimens putatively identified as M. quinquefasciata collected ... more

J.O.P. Pereira; B.M. Freitas; D.M.M. Jorge; D.C. Torres; C.E.A. Soares; T.B. Grangeiro
Aspergillus flavus; Genetic diversity; Inter-simple sequence repeats; Internal transcribed spacer; Molecular markers; Random amplified polymorphic DNA

The Aspergillus genus belongs to a filamentous fungal group characterized by wide dispersion in the environment. Some species are associated with diseases, especially in immunocompromised patients, while others are of economical importance due to aflatoxin production or biotechnological applications. Its species identification is nowadays performed by traditional techniques combined ... more

P.P. Batista; J.F. Santos; N.T. Oliveira; A.P.D. Pires; C.M.S. Motta; E.A.LunaAlves Lima
Internal transcribed spacer; nuclear ribosomal DNA; Phylogenetic relationships; Phytolaccaceae

The phylogeny of a phylogenetically poorly known family, Phytolaccaceae sensu lato (s.l.), was constructed for resolving conflicts concerning taxonomic delimitations. Cladistic analyses were made based on 44 sequences of the internal transcribed spacer of nuclear ribosomal DNA from 11 families (Aizoaceae, Basellaceae, Didiereaceae, Molluginaceae, Nyctaginaceae, ... more

J. Lee; S.Y. Kim; S.H. Park; M.A. Ali
India; Internal transcribed spacer; Invasion management; Kashmir Himalaya; Molecular authentication; Myriophyllum aquaticum

Myriophyllum aquaticum (Vell.) Verdc. (family Haloragaceae) is one of the most invasive and destructive South American aquatic plant species and is present in a wide range of geographic regions, including the Kashmir Himalaya. Confusion regarding the taxonomic delimitation of M. aquaticum in the Himalayan region impedes effective and targeted management. Hence, our goal ... more

M.A. Shah; M.A. Ali; F.M. Al-Hemaid; Z.A. Reshi
Dendrobium; Internal transcribed spacer; Molecular marker; Simple sequence repeat

The Orchidaceae is one of the largest and most diverse families of flowering plants. The Dendrobium genus has high economic potential as ornamental plants and for medicinal purposes. In addition, the species of this genus are able to produce large crops. However, many Dendrobium varieties are very similar in outward appearance, making it difficult to distinguish one ... more

Y.T. Liu; R.K. Chen; S.J. Lin; Y.C. Chen; S.W. Chin; F.C. Chen; C.Y. Lee
Calendula; Colchicine; Flowering yield; Molecular diversity; Mutation; SRAP

Six different colchicine concentrations: 0, 400, 800, 1200, 1600, and 2000 ppm, in combination with four soaking time treatments (1, 2, 3, and 4 h), were selected to assess the effects on germination, vegetative growth, and flower yield components in calendula plants. The molecular diversity among the treatments was assessed using ten SRAP marker combinations. Seed soaking in colchicine ... more

Y.I. El-Nashar; M.H. Ammar; Y.I. El-Nashar; M.H. Ammar; Y.I. El-Nashar; M.H. Ammar
Bertholletia excels; Conservation; Genetic resources; Molecular diversity

Brazil nut is a native Amazon species of high commercial value classified as vulnerable in terms of extinction risk due to marked illegal-burning activity and agricultural-frontier expansion processes occurring in the region. This study was undertaken to analyze the genetic diversity within and between two natural Brazil-nut populations occurring spontaneously ... more

F.S. Vieira; A.A. Rossi; G.F. Pena; A.V. Tiago; K.É.M. Zortéa; K.F.M. Schmitt; L.D. Giustina; F.S. Rossi; P.H.A.D. Santos
DNA barcoding; Internal transcribed spacer; matK; Orinus; rbcL; trnH-psbA

Orinus is an alpine endemic genus of Poaceae. Because of the imperfect specimens, high level of intraspecific morphological variability, and homoplasies of morphological characters, it is relatively difficult to delimitate species of Orinus by using morphology alone. To this end, the DNA barcoding has shown great potential in identifying species. The present study is the ... more

X. Su; Y.P. Liu; Z. Chen; K.L. Chen; X. Su; Y.P. Liu; Z. Chen; K.L. Chen; X. Su; Y.P. Liu; Z. Chen; K.L. Chen
Biomass depolymerization; Internal transcribed spacer; Xylanolytic enzymes

Disordered anthropic action causes relevant impacts on different ecosystems. This may endanger key species and the compounds they produce, which have potential for commercial development. We collected environmental samples from the Bela Vista Biological Refuge, belonging to ITAIPU/Brazil, over a period of three years. A total of 181 fungal species were isolated and ... more

J.M. Corrêa; S.S. de Moraes; C. Henn; M.K. Kadowaki; J.Lda C. Silva; A. Maller; deC.G. Simão