Research Article

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Brontispa longissima is one of the most serious insect pests of coconut in Southeast Asia; it was first discovered on Hainan Island in June 2002. Despite the economic risk associated with this pest, genetic aspects of the invasion process have remained relatively unexplored. Using microsatellite markers, we investigated the population structure, genetic variability and pattern of ... more

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Nelumbo nucifera is an important economic vegetable and traditional medicine, but available genetic resources remain limited. Next generation sequencing has proven to be a rapid and effective means of identifying genic simple sequence repeat (genic-SSR) markers. This study developed genic-SSRs for N. nucifera using Illumina sequencing technology to assess diversity across ... more

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Heat shock protein; high temperature; Transcriptome; Tree peony leaf

The tree peony leaf is an important vegetative organ that is sensitive to abiotic stress and particularly to high temperature. This sensitivity affects plant growth and restricts tree peony distribution. However, the transcriptomic information currently available on the peony leaf in public databases is limited. In this study, we sequenced the transcriptomes of peony leaves subjected to ... more

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Eleocharis dulcis; High-throughput sequencing; Simple sequence repeat; Transcriptome

Water chestnut (Eleocharis dulcis) is an important aquatic crop in China; however, transcriptomic and genomic data in public databases are limited. To identify genes and development molecular markers, high-throughput transcriptome sequencing was applied to generate transcript sequences from water chestnut leaf. More than 24 million reads were obtained, trimmed, and assembled into ... more

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Chemoreception; Expression pattern; Rhynchophorus ferrugineus; Transcriptome

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Acipenser schrenckii; Ovaries; Reproduction; Testes; Transcriptome

Sturgeons (Acipenser schrenckii) are of high evolutionary, economic, and conservation value, and caviar isone of the most valuable animal food products in the world. The Illumina HiSeq2000 sequencing platform was used to construct testicular and ovarian transcriptomes to identify genes involved in reproduction and sex determination in A. schrenckii. A total of 122,381 ... more

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Cocoa tea; Theobromine biosynthesis; Trans-catechins biosynthesis; Transcriptome

Camellia ptilophylla, or cocoa tea, is naturally decaffeinated and its predominant catechins and purine alkaloids are trans-catechins and theobromine Regular tea [Camellia sinensis (L.) O. Ktze.] is evolutionarily close to cocoa tea and produces cis-catechins and caffeine. Here, the transcriptome of C. ptilophylla was sequenced using the 101- ... more

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Alkalinity stress; Carbonate; Nile tilapia; Transcriptome

To identify genes of potential importance to alkalinity tolerance, RNA sequencing (RNA-Seq) was performed to survey gill transcriptome profiles from freshwater (FW) and alkaline water (AW) exposed Nile tilapia (Oreochromis niloticus). A total of 22,724,036 (AW)/16,461,040 (FW) single-end reads were generated in which 20,304,348 (AW)/14,681,290 (FW) reads (90.0/89.72%) were ... more

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Laying; Pituitary gland; Prelaying; Sichuan white geese; Transcriptome

To better understand the molecular mechanism(s) underlying egg-laying in Sichuan white geese, the profiles of genes in the pituitary gland were investigated during the prelaying and laying periods. Total RNA was extracted from the pituitary glands of geese during prelaying or laying periods and cDNA was generated. After sequencing and annotation, 54 upregulated and 84 downregulated genes were ... more

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