Research Article

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12/08/2011
Lactation; Leptin; Pregnancy; Rat; Virgin

Leptin is expressed in various tissues, suggesting that this protein is effective not only at the central nervous system level, but also peripherically. Recent studies have shown leptin production by other tissues, including the placenta, stomach, and mammary tissues, but there is no information available concerning expression levels of leptin in the rat mammary gland at different activation ... more

Y.Y. Wang; Y.L. Wang; H.P. Li; H.S. Zhu; Q.D. Jiang; L. Zhang; L.F. Wang; L.Q. Han; K. Zhong; Y.J. Guo; W.F. Lu; H.J. Li; G.Y. Yang
04/12/2011
Lactation; Mouse; Pregnancy; PrRP

We compared levels of prolactin-releasing peptide (PrRP) mRNA expression in mouse medulla at different stages of pregnancy and lactation. Mouse medulla samples were collected on days 6, 12 and 18 of pregnancy and lactation, respectively (six per group), for mRNA. Expression levels of PrRP mRNA in the medulla were measured by semi-quantitative RT-PCR, with glyceraldehyde 3-phosphate ... more

H.S. Zhu; J.X. Du; Y.Y. Wang; L.F. Wang; L.Q. Hang; G.Y. Yang; Y.L. Wang
06/29/2010
Isoform; Lactation; Lipogenic gene; Mouse mammary gland; Quantitative real-time PCR

The functions of distinct isoforms of solute carrier family 27 transporters (SLC27A1-6), acetyl-CoA carboxylase (ACACA, ACACB), stearoyl-CoA desaturase (SCD1-4), fatty acid desaturase (FADS1-3), LPIN (LPIN1-3), insulin-induced gene (INSIG1, 2), and peroxisome proliferator-activated receptor gamma coactivator1 (PPARGC1A, B) were studied in the mouse mammary gland from pregnancy to ... more

L.Q. Han; H.J. Li; Y.Y. Wang; H.S. Zhu; L.F. Wang; Y.J. Guo; W.F. Lu; Y.L. Wang; G.Y. Yang
03/16/2010
geNorm; Lactation; Quantitative real-time PCR; Reference gene

Obtaining quantitative data concerning gene expression is important for understanding milk synthesis in mammary glands. Quantitative real-time PCR (qRT-PCR) is an efficient tool to calculate gene expression; however, it is necessary to find valid reference genes for normalization of qRT-PCR data. We applied the geNorm software to eight commonly used reference genes to identify the most ... more

L.Q. Han; G.Y. Yang; H.S. Zhu; Y.Y. Wang; L.F. Wang; Y.J. Guo; W.F. Lu; H.J. Li; Y.L. Wang
12/21/2012
IL-8; Lactation; Mammary gland; Mouse; Pregnancy

Interkeukin-8 (IL-8) is an important inflammatory mediator. It is an angiogenic factor associated with inflammation and carcinogenesis. To date, research on IL-8 has been limited to its role as an indicator of inflammation. There has been no systematic research concerning IL-8 expression levels in the mouse mammary gland during pregnancy and lactation. Mouse mammary gland samples were ... more

H.P. Li; Y.J. Guo; H.S. Zhu; K. Zhong; G.M. Zha; L.F. Wang; Y.L. Wang; W.F. Lu; Y.Y. Wang; G.Y. Yang
07/02/2014
Lactation; Lactoferrin; Mammary gland; Mouse; Pregnancy

Lactoferrin (Lf) is an iron-binding glycoprotein that is produced by mucosal epithelial cells in mammals. Lf has non-immune natural defense functions and biological functions in addition to and distinct from its role in regulating inflammatory responses. Lf also improved some physiological and immunological parameters. Lf is a biomarker for monitoring medical treatment in inflammatory ... more

H.P. Li; X.L. Jiao; X.J. Wang; F.T. Liu; H.S. Zhu; Y.Y. Wang; G.Y. Yang
03/27/2015
Chapman model; Feeding program; Growth; Lactation; Survival rate

Giant panda cubs have a low survival rate during the newborn and early growth stages. However, the growth and developmental parameters of giant panda cubs during the early lactation stage (from birth to 6 months) are not well known. We examined the growth and development of giant panda cubs by the Chapman growth curve model and estimated the heritability of the maximum growth rate at the ... more

T.D. Che; C.D. Wang; L. Jin; M. Wei; K. Wu; Y.H. Zhang; H.M. Zhang; D.S. Li
11/13/2015
Giant panda; Lactation; Mammary gland; miRNA

The giant panda (Ailuropoda melanoleuca) is one of the world’s most endangered mammals, and it has evolved several unusual biological and behavioral traits. During puberty, pregnancy, lactation, and involution, the mammary gland undergoes profound morphological and functional changes. A large number of microRNAs (miRNAs) have been identified to be involved in mammary gland ... more

C.D. Wang; K. Long; L. Jin; S. Huang; D.H. Li; X.P. Ma; M. Wei; Y. Gu; J.D. Ma; H. Zhang
3/22/2017
Actins; Animal Feed; Animals; Cattle; Chemokine CCL5; Diet; Female; Interleukin-8; Lactalbumin; Lactation; Mammary Glands, Animal; Milk; Milk proteins; Random Allocation; Rumen

In order to evaluate the milk yield, milk quality, and health of dairy cows fed a high-concentrate (HC) diet, eight lactating Holstein dairy cattle were randomly assigned to HC or low-concentrate (LC) diet groups and fed for 50 days, and the auto-control studying before and after treatment with the two diets was used. During the experiment, plasma and milk samples were collected and measured. ... more

Z.L. Xie; J. Zhang; D.M. Zhang; J.F. Li; Y.H. Lin
06/29/2017
Animals; Cattle; Female; Genome-wide association study; Genotype; Lactation; Longevity; Milk; Quantitative trait loci; Quantitative Trait, Heritable; Reproduction; Selective breeding

In this study, we estimated the genetic parameters and identified the putative quantitative trait loci (QTL) associated with the length of productive life (LPL), days open (DO), and 305-day milk yield for the first lactation (FM305) of crossbred Holstein dairy cattle. Data comprising 4,739 records collected between 1986 and 2004 were used to estimate the variance-covariance components using ... more

P. Saowaphak; M. Duangjinda; S. Plaengkaeo; R. Suwannasing; W. Boonkum

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