Research Article

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blood; Bovine papillomavirus; Milk; semen; spermatozoa; urine

Papillomavirus infection in bovines is associated with cutaneous papillomatosis on the hide, udders and other epithelial tissues, as well as in oral respiratory, alimentary and urinary tract mucosa. Bovine papillomavirus (BPV) is also considered the etiological agent of esophageal tumors and the malignant bladder tumors that characterize the clinical condition associated with chronic enzootic ... more

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Bovine leukemia virus; Bovine papillomavirus; Chromosome aberrations; Virus co-infection

Bovine papillomavirus (BPV) DNA sequences were detected in different tissues, in addition to epithelium. Cytogenetic abnormalities were observed in blood lymphocytes. The presence of more than one virus in a single tissue is a difficult aspect to evaluate,especially when the DNA sequences are detected in tissues that are not specifically targeted by the virus. BPV and bovine leukemia virus ( ... more

A. Yaguiu; M.L.Z. Dagli; E.H. Birgel; B.C.A.Alves Reis; O.P. Ferraz; E.M. Pituco; A.C. Freitas; W. Beçak; R.C. Stocco
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Bovine papillomaviruses (BPVs) cause many benign and malignant lesions in cattle and other animals. Twelve BPV types have been identified so far, and several putative novel BPV types have been detected based on the analysis of L1 gene fragments, generated by FAP59/64 and MY11/09 primers. Phylogenetic trees are important in studies that describe novel BPV types. However, topological ... more

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Papillomaviruses are known to cause benign or malignant lesions in various animals. In cattle, bovine papillomavirus (BPV) is the etiologic agent of papillomatosis and neoplasia of the upper gastrointestinal tract and urinary bladder. Currently, there are no standard diagnostic tests or prophylactic vaccines. Protection against papillomavirus infection is conferred by neutralizing ... more

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Bovine papillomavirus (BPV) is an oncogenic virus associated with benign and malignant lesions, which result in notable economic losses. Peripheral blood samples and cutaneous papillomas were obtained from four adult beef cattle. Viral molecular identification was performed using specific primers for BPV-1, -2 and -4 in blood diagnosis and FAP59/FAP64 for skin papillomas. Histopathologic ... more

R.P. Araldi; R.F. Carvalho; T.C. Melo; N.S.P. Diniz; T.A. Sant’Ana; J. Mazzuchelli-de-Souza; D.D. Spadacci-Morena; W. Beçak; R.C. Stocco
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Bovine papillomaviruses (BPVs) are recognized as causal agents of benign and malignant tumors in cattle. Thirteen types of BPVs have already been described and classified into 3 distinct genera. Divergences in the nucleotide sequence of the L1 gene are used to identify new viral types through the employment of PCR assays with degenerated primers. In the present study, a method for ... more

T.C. Melo; R.F. Carvalho; J. Mazzucchelli-de-Souza; N. Diniz; S. Vasconcelos; S.L.M.R. Assaf; R.P. Araldi; R.M. Ruiz; I. Kerkis; W. Beçak; R.C. Stocco
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Bovine papillomavirus; BPV; DNA vaccine

Papillomaviruses are known to cause tumor lesions, generally benign, in epithelial tissues of diverse organisms; these lesions may progress to cancer under suitable conditions. Bovine papillomavirus (BPV) can cause urinary bladder cancer and cancer of the upper gastrointestinal tract. Furthermore, BPV1 and BPV2 are implicated in the development of tumors in equids. Many studies with ... more

E.G. Lima; R.C. Lira; A.L.S. Jesus; R. Dhalia; A.C. Freitas
10/21/2015
Bovine papillomavirus; Comet assay; Histopathology; Immunodetection; Viraloncogenesis

The bovine papillomavirus (BPV) causes papillomas that regress spontaneously, but can also progress to malignancy. This study evaluated the role of BPV in oncogenesis. Twenty-four samples from uninfected calves and the papillomas of BPV infected cattle were subjected to molecular diagnosis, as well as histopathological and immunohistochemical analyses. The comet assay (CA) was used to ... more

R.P. Araldi; T.C. Melo; A.C. Neves; D.D. Spadacci-Morena; R.F. Magnelli; D.G. Modolo; P.L. de-Sá-Júnior; J. Mazucchelli-de-Souza; R.F. Carvalho; W. Beçak; R.C. Stocco
10/21/2008
Bovine papilloma virus; cutaneous papillomatosis; Papillomavirus; primary cell cultures; wart

Papillomaviruses have been reported to be very difficult to grow in cell culture. Also, there are no descriptions of cell cultures from lesions of bovine cutaneous papillomatosis, with identification of different bovine papilloma virus (BPV) DNA sequences. In the present report, we describe primary cell cultures from samples of cutaneous lesions (warts). We investigated the simultaneous ... more

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