Research Article

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07/14/2015
Database; EST; Evolution; Nicotiana; Primer; Single nucleotide polymorphism

Single nucleotide polymorphisms (SNPs) are widespread in the Nicotiana genome. Using an alignment and variation detection method, we developed 20,607,973 SNPs, based on the expressed sequence tag sequences of 10 Nicotiana species. The replacement rate was much higher than the transversion rate in the SNPs, and SNPs widely exist in the Nicotiana. In vitro ... more

Y. Wang; D. Zhou; S. Wang; L. Yang
10/19/2015
EST; Expressing; Piper hainanense

Black pepper is a perennial climbing vine. It is widely cultivated because its berries can be utilized not only as a spice in food but also for medicinal use. This study aimed to construct a standardized, high-quality cDNA library to facilitated identification of new Piper hainanense transcripts. For this, 262 unigenes were used to generate raw reads. The average length of these ... more

R. Fan; P. Ling; C.Y. Hao; F.P. Li; L.F. Huang; B.D. Wu; H.S. Wu
07/15/2016
EST; Microsatellite; Populus cathayana; Qinghai-Tibet Plateau

Populus cathayana occupies a large area within the northern, central, and southwestern regions of China, and is considered to be an important reforestation species in western China. In order to investigate the population genetic structure of this species, 10 polymorphic microsatellite loci were identified based on expressed sequence tags from de novo sequencing on the ... more

Z.Z. Tian; F.Q. Zhang; Z.Y. Cai; S.L. Chen; Z.Z. Tian; F.Q. Zhang; Z.Y. Cai; S.L. Chen
12/11/2015
Bioinformatics; Clone; LOC339524 gene; Sprague-Dawley rat (Rattus norvegicus)

We cloned the LOC339524 gene in Sprague-Dawley (SD) rats and analyzed the structure and function of the protein encoded by it. Based on the known human LOC339524 gene sequences, the full-length coding sequence of the LOC339524 gene in SD rats was cloned and amplified by the polymerase chain reaction using the complementary DNA of SD rats as a template. Bioinformatics analysis showed that ... more

Z.H. Long; H. Li; F. Chen; L.Y. Zou
10/30/2015
Bacteria; Bioinformatics; Fungi; Laccase; Oomycetes; Plant pathogen

To date, research on laccases has mostly been focused on plant and fungal laccases and their current use in biotechnological applications. In contrast, little is known about laccases from plant pathogens, although recent rapid progress in whole genome sequencing of an increasing number of organisms has facilitated their identification and ascertainment of their origins. In this study, a ... more

B.Z. Feng; P.Q. Li; L. Fu; X.M. Yu
10/28/2015
Alveolar bone cell; Bioinformatics; Biological pathway; Diabetes

Alveolar bone osteoblasts are widely used in dental and related research. They are easily affected by systemic diseases such as diabetes. However, the mechanism of diabetes-induced alveolar bone absorption remains unclear. This study systematically explored the changes in human alveolar bone cell-related gene expression and biological pathways, which may facilitate the investigation of ... more

X. Sun; Q.H. Ren; L. Bai; Q. Feng
10/16/2015
Bioinformatics; Chordates; Evolutionary biology; Myogenic regulatory factors

MyoD and MyoG are transcription factors that have essential roles in myogenic lineage determination and muscle differentiation. The purpose of this study was to compare multiple amino acid sequences of myogenic regulatory proteins to infer evolutionary relationships among chordates. Protein sequences from Mus musculus (P10085 and P12979), human Homo sapiens (P15172 and ... more

L.C. Oliani; K.C.F. Lidani; J.E. Gabriel
03/28/2016
Bioinformatics; Identification; MicroRNA; Middleware; RNA sequencing; Web server

This report describes the miRQuest - a novel middleware available in a Web server that allows the end user to do the miRNA research in a user-friendly way. It is known that there are many prediction tools for microRNA (miRNA) identification that use different programming languages and methods to realize this task. It is difficult to understand each tool and apply it to diverse datasets ... more

R.R. Aguiar; L.A. Ambrosio; G. Sepúlveda-Hermosilla; V. Maracaja-Coutinho; A.R. Paschoal; R.R. Aguiar; L.A. Ambrosio; G. Sepúlveda-Hermosilla; V. Maracaja-Coutinho; A.R. Paschoal; R.R. Aguiar; L.A. Ambrosio; G. Sepúlveda-Hermosilla; V. Maracaja-Coutinho; A.R. Paschoal
06/18/2019
Bioinformatics; Conserved domains; Homology modeling

The Allium genus stands out for its uses in human food and also for its medicinal properties. Many representatives of the Amaryllidaceae family are known for producing mannose binding lectins (MBL). In plants, lectins act as reserves of proteins that can be used for plant growth and development and also in defense against herbivores and pathogens, being ... more

H.J. Jimenez; L.S.S. Martins; R. Carvalho; A.V.V. Montarroyos; R.M.Moraes Filho
09/25/2015
Colostrum; Goat; Mature milk; MicroRNA

Immune-related miRNAs in breast milk are extracellular miRNAs that are related to immune organ development and regulation of the immune function in infants and young animals. The goal of this study was to compare the expression levels of five immune-related miRNAs in breast milk in black goats, humans, and dairy cattle. The miRNAs from milk were extracted and the expression levels were ... more

R.S. Na; G.X. E; W. Sun; X.W. Sun; X.Y. Qiu; L.P. Chen; Y.F. Huang

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