Research Article

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06/20/2013
Apis cerana; Genetic differentiation; Genetic diversity; mtDNA

DNA sequence diversity in the tRNAleu-COII portion of the mitochondrial genome was investigated in samples of Apis cerana from Yunnan, China. A fragment of about 480 bp in tRNAleu-COII, including a noncoding area and part of COII, was sequenced. The noncoding area was 97-98 bp; 8 haplotypes were found, among which 5 had been reported previously, while 3 ... more

L. Yin; T. Ji
10/31/2014
Apis cerana; Gene expression; Transcriptome; Varroa destructor

Varroa destructor is the greatest threat to the honeybee Apis mellifera worldwide, while it rarely causes serious harm to its native host, the Eastern honeybee Apis cerana. The genetic mechanisms underlying the resistance of A. cerana to Varroa remain unclear. Thus, understanding the molecular mechanism of resistance to Varroa may ... more

T. Ji; L. Yin; Z. Liu; F. Shen; J. Shen
10/31/2014
Apis cerana; Gene expression; Transcriptome; Varroa destructor

The Varroa destructor mite has become the greatest threat to Apis mellifera health worldwide, but rarely causes serious damage to its native host Apis cerana. Understanding the resistance mechanisms of eastern bees against Varroa mites will help researchers determine how to protect other species from this organism. The A. cerana genome has not ... more

T. Ji; L. Yin; Z. Liu; Q. Liang; Y. Luo; J. Shen; F. Shen
09/23/2016
Apis cerana; Chinese sacbrood virus; Genome sequence; Honeybee

The complete genomic RNA of the Chinese sacbrood virus (CSBV) strain, which infects the honeybees in the Loess plateau, was sequenced and analyzed. The CSBV-SX strain contains 8705 nucleotides, which includes a single large open reading frame (99-8681 nucleotides) encoding 2860 amino acids. A novel efficient identification method was used to investigate the samples infected by CSBV. The ... more

H. Yu; T.X. Liu; D. Wang; H. Yu; T.X. Liu; D. Wang
09/23/2016
Differential expression; Energy metabolism; Temperature; Varroa destructor

Varroa destructor mites pose an increasing global threat to the apicultural industry and agricultural ecology; however, the issue of whether certain environmental factors reflect the level of mite infection is far from resolved. Here, a wireless sensor network (WSN) system was used to examine how V. destructor, which has vital impacts on honeybee (Apis mellifera ... more

C.S. Hou; B.B. Li; S. Deng; Q.Y. Diao; C.S. Hou; B.B. Li; S. Deng; Q.Y. Diao
07/29/2016
Differential expression; Dual Bt gene; Genetic transformation; Insect-resistance; Plant expression vector; Tobacco

This study aimed to determine the influence of vector structure on dual Bt gene expression and establish an efficient expression vector using Cry1Ac and Cry3A genes. Four vectors (N4, N5, N10, and S23) were developed and used for genetic transformation of tobacco to obtain insect-resistant transgenic lines. The vectors were constructed using the MAR structure, ... more

L.N. Xu; Y. Dong; J. Zhang; R.X. Wang; H.M. Liu; Q. Yang; M.S. Yang; L.N. Xu; Y. Dong; J. Zhang; R.X. Wang; H.M. Liu; Q. Yang; M.S. Yang
11/03/2016
Cis-elements; Differential expression; Low temperature; Oryza sativa L.; Reactive oxygen species; Salinity

Different rice (Oryza sativa L.) genotypes were subjected to high salinity and low temperature (150 mM NaCl and 13°C, respectively) for 0, 6, 24, 48, or 72 h. We evaluated the simultaneous expression of the genes OsCATA, OsCATB, and OsCATC, correlated gene expression with enzyme activity, and verified the regulation of these ... more

I.L. Vighi; L.C. Benitez; M.Ndo Amaral; P.A. Auler; G.P. Moraes; G.S. Rodrigues; L.C. da Maia; L.S. Pinto; E.J.B. Braga; I.L. Vighi; L.C. Benitez; M.Ndo Amaral; P.A. Auler; G.P. Moraes; G.S. Rodrigues; L.C. da Maia; L.S. Pinto; E.J.B. Braga
11/30/2015
Ecology; Flue-cured tobacco; Leaf quality; Proteome; Two-dimensional electrophoresis

The leaves of tobacco plants were used to analyze differences in protein content of tobacco grown in the four main flue-cured tobacco-producing areas of Sichuan Province, China. An improved protein extraction method, isoelectric focusing/sodium dodecyl sulfate-polyacrylamide gel electrophoresis two-dimensional gel electrophoretic separation, was used to extract and separate total protein from ... more

L.M. Lu; K.Y. Ye; Z.X. Tang; L. Liu; Y. Chen; J. Luo; Y.B. Huang
05/13/2016
Mass spectrometry; Mycelium; Nitrogen stress; Proteome; Rice blast fungus

Magnaporthe oryzae is an important model system in studies of plant pathogenic fungi, and nitrogen is a key nutrient source affecting microbial growth and development. In order to understand how nitrogen stress causes changes in mycelial proteins, we analyzed differentially expressed mycelial proteins from the M. oryzae virulent strain CH-63 using two-dimensional ... more

X.G. Zhou; P. Yu; C. Dong; C.X. Yao; Y.M. Ding; N. Tao; Z.W. Zhao; X.G. Zhou; P. Yu; C. Dong; C.X. Yao; Y.M. Ding; N. Tao; Z.W. Zhao
5/25/2017
Disease resistance; Fusarium; Gene Expression Regulation, Plant; Lycopersicon esculentum; Plant Proteins; Plant Roots; Proteome; Stress, Physiological

Fusarium wilt caused by Fusarium oxysporum f. sp lycopersici (Fol) is one of the main diseases affecting tomatoes. The BHRS 2,3 genotype of tomato is, however, resistant to this disease. A proteomic approach was used to understand the defense mechanisms of this genotype using the tomato root, the first tissue that interacts with the fungus, as a target. Protein was extracted and separated by ... more

T.D. Silva; C.M.A. Almeida; C.B. Malafaia; L.M.S. Oliveira; M.V. Silva; M.T.S. Correia

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