Research Article

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DNA microarray; Gastric cancer cells; Gene expression; Tumor spheres

The purpose of this study was to screen for genes that were differentially expressed between a human gastric carcinoma cell line (HGC-27) and their tumor spheres, using the gene chip technique. The HGC-27 cells and tumor sphere cells were cultured in vitro in a sterile environment. Total RNA was extracted from both samples and purified using a standard TRIzol reagent. Total RNA ... more

W.H. Shi; Y.B. Yu; Z.Y. Li; X.C. Guo; Z.L. Wei; Z.H. Zheng
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Gene expression; H22 hepatoma; Luteolin; Tumor tissue

The purpose of our study was to observe the effects of luteolin on the expression of the genes ICAM-1, LFA-3, and PCNA in H22 hepatoma tissue. Sixty ICR (Institute of Cancer Research) mice with H22 hepatoma were randomly divided into five groups: a normal saline control group, low-, medium-, and high-dose luteolin groups, and a cyclophosphamide group. The mice ... more

J.X. Niu; H.P. Guo; H.M. Gan; L.D. Bao; J.J. Ren
10/16/2015
Cloning; Gene expression; Insulin-like growth factor-binding protein; Nanjiang brown goat

Insulin-like growth factor-binding proteins (IGFBPs) play a key role in modulating insulin-like growth factors (IGFs), and are considered candidate genes for growth traits in livestock. In this study, we identified the complete coding sequences of IGFBP-1 to -6 in the Nanjiang brown goat, and assessed gene tissue expression patterns by quantitative polymerase chain ... more

S.Y. Zhan; L. Chen; L. Li; L.J. Wang; T. Zhong; H.P. Zhang
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Embryonic stem cell; Fibroblast; Gene expression; Induced pluripotent stem cell

We investigated gene expression in embryonic stem (ES) cells, induced pluripotent stem (iPS) cells, and fibroblasts. Microarray expression data sets obtained from the Gene Expression Omnibus were analyzed using the Partek software. Human genes from ES cells, iPS cells, and fibroblasts were ranked from low to high according to their expression levels. The gene expression mode in iPS cells ... more

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Brassica rapa ssp pekinensis; Gene expression; Gene structure; sHSP

Small heat shock proteins (sHSPs) are essential for the plant’s normal development and stress responses, especially the heat stress response. The information regarding sHSP genes in Chinese cabbage (Brassica rapa ssp pekinensis) is sparse, hence we performed a genome-wide analysis to identify sHSP genes in this species. We identified 26 non-redundant sHSP genes ... more

P. Tao; W.L. Guo; B.Y. Li; W.H. Wang; Z.C. Yue; J.L. Lei; X.M. Zhong
02/19/2016
Gene expression; peanut; Pod development; SQUAMOSA promoter-binding protein-like protein

SQUAMOSA promoter-binding protein-like (SPL) proteins play crucial roles in plant growth, development, and responses to environmental stressors. The peanut (Arachis hypogaea L.) is a globally important oil crop. In this study, we cloned the full-length cDNA of 15 SPLs in the peanut by transcriptome sequencing and rapid amplification of cDNA ends, and analyzed their ... more

M. Li; S.Z. Zhao; C.Z. Zhao; Y. Zhang; H. Xia; J. Lopez-Baltazar; S.B. Wan; X.J. Wang; M. Li; S.Z. Zhao; C.Z. Zhao; Y. Zhang; H. Xia; J. Lopez-Baltazar; S.B. Wan; X.J. Wang
10/24/2016
Bioinformatic analysis; Gene expression; MYB10; Red-leaf peach

In higher plants, the transcription factor MYB10 is an important regulator of anthocyanin biosynthesis. In order to study its role in the development of red coloration in peach leaves, the full-length MYB10 complementary DNA sequence of the red-leaf peach cultivar ‘Tsukuba No. 5’ (Prunus persica f. atropurpurea) was successfully cloned ... more

X.Q. Wang; J. Han; Y. Wen; W.B. Jiang; J.G. Fang; B.B. Zhang; R.J. Ma; X.Q. Wang; J. Han; Y. Wen; W.B. Jiang; J.G. Fang; B.B. Zhang; R.J. Ma
12/02/2016
Animals; Avian Proteins; Body weight; Breeding; Chickens; Female; Gene expression; Gene Expression Regulation; male; Muscle development; Myostatin; Organ Specificity; Phenotype; Quantitative trait loci

Myostatin (MSTN) is expressed in the myotome and developing skeletal muscles, and acts to regulate the number of muscle fibers. Wuding chicken large body, developed muscle, high disease resistance, and tender, delicious meat, and are not selected for fast growth. Broiler chickens (Avian broiler) are selected for fast growth and have a large body size and high muscle mass. Here, 240 one-day-old ... more

L.X. Liu; T.F. Dou; Q.H. Li; H. Rong; H.Q. Tong; Z.Q. Xu; Y. Huang; D.H. Gu; X.B. Chen; C.R. Ge; J.J. Jia
12/02/2016
Deoxyribonucleases; DNA; Gene expression; Plant Leaves; RNA, Plant; Saccharum

The success of gene expression studies, protein synthesis, and construction of cDNA libraries directly depends on the purity and integrity of the RNA used in these tests, as even minor amounts of contaminant DNA (<1%) can produce a false positive amplification signal in quantitative real-time PCR. For RNA contaminated with genomic DNA, an essential step in the studies on gene expression is ... more

J.A. Santos; G.A. Luz; K.P. Oliveira; L.F. Oliveira; A.S.Andrade Júnior; S.E.S. Valente; P.S.C. Lima
12/19/2016
Athletes; Cell Hypoxia; Cell proliferation; Cells, Cultured; Endothelial progenitor cells; Exercise; Gene expression; Humans; Lipoproteins; Skiing

In this study, we compared the functional properties of endothelial progenitor cells (EPCs) derived from halfpipe-snowboarding athletes who train under hyperoxic conditions with those derived from normal subjects who lived under normoxic conditions. Peripheral blood-derived EPCs were isolated from both halfpipe-snowboarding athletes and normal humans. Cellular growth dynamics, lipoprotein ... more

Y.H. Zhao; J.C. Kan; Y.F. Wang; W.J. Guan; Z.Q. Zhu

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