Research Article

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08/03/2010
DNA; DYS464; Microsatellites; Population genetics; Worldwide populations; Y-chromosome

Of all DNA markers on the human Y-chromosome, the tetra-local Y-linked microsatellite DYS464 is the most polymorphic. We genotyped DYS464 in 677 male samples collected worldwide, maintained in the HGDP-CEPH Human Genome Diversity Cell Line Panel. Fourteen different alleles were found, with allele lengths varying from 9 to 23 repeats. One hundred and seventy-five ... more

F.S.G. Kehdy; S.D.J. Pena
02/09/2010
Electrophoretograms; Genetic diversity; Microsatellites; Molecular markers; Stutter peaks

Microsatellite markers are commonly used for examining population structure, especially inbreeding, outbreeding and gene flow. An array of microsatellite loci, preferably with multiallelic presentation, is preferable for ensuring accurate results. However, artifact peaks or stutters in the electrophoretograms significantly hamper the reliable interpretation of genotypes. We interpreted ... more

I.A. Arif; H.A. Khan; M. Shobrak; A.A.Al Homaidan; A. Sadoon; A.H.Al Farhan; A.H. Bahkali
02/28/2013
Linkage analysis; Microsatellites; Mystus nemurus; Pseudo-testcross strategy; River catfish; Segregation

The river catfish Mystus nemurus is an important fresh water species for aquaculture in Malaysia. We report the first genetic linkage map of M. nemurus based on segregation analysis and a linkage map using newly developed microsatellite markers of M. nemurus. A total of 70 of the newly developed polymorphic DNA microsatellite markers were analyzed on pedigrees ... more

B.P. Hoh; S.S. Siraj; S.G. Tan; K. Yusoff
04/27/2012
genetic breeding; Microsatellites; Musa spp; Primer validation

Thirty-four microsatellite markers (SSRs) were identified in EST and BAC clones from Musa acuminata burmannicoides var. Calcutta 4 and validated in 22 Musa genotypes from the Banana Germplasm Bank of Embrapa-CNPMF, which includes wild and improved diploids. The number of alleles per locus ranged from 2 to 14. The markers were considered highly informative based on their ... more

E.P. Amorim; P.H. Silva; C.F. Ferreira; V.B.O. Amorim; V.J. Santos; A.D. Vilarinhos; C.M.R. Santos; M.T.Souza Júnior; R.N.G. Miller
02/17/2012
1711b satellite; Cattle; indicus; Microsatellites; mtDNA; taurus

Our objective was to estimate Bos primigenius taurus introgression in American Zebu cattle. One hundred and four American Zebu (Nellore) cattle were submitted to mtDNA, microsatellite and satellite analysis. Twenty-three alleles were detected in microsatellite analysis, averaging 4.6 ± 1.82/locus. Variance component comparisons of microsatellite allele sizes allowed the ... more

P. Ripamonte; M. Baccaglini; A.S.M. César; A.S.M. César; L.G.G. Figueiredo; J.C.C. Balieiro; A.R. Caetano; F.V. Meirelles
02/10/2012
Colombia; Microsatellites; Molecular characterization; Rubus glaucus; SSRs; Thornless blackberry

The species Rubus glaucus, also known as the Andean or “Castilla” blackberry, is one of nine edible species of this genus that grow naturally in Central and South America. In Colombia, this species is the most important of all Rubus species for agricultural and commercial purposes. We used 20 SSRs developed for other Rubus species to characterize 44 Colombian ... more

M. Marulanda; A.M. López; M. Uribe
10/31/2014
Medicinal plant; Microsatellites; Population genetics; Tetrastigma hemsleyanum

Tetrastigma hemsleyanum (Vitaceae) is an endangered medicinal plant endemic to China. Because of its widely known efficacy for treating many health problems, wild resources of this species are currently undergoing a rapid decline. Few studies have been conducted examining the population genetics or development of microsatellite loci for this plant. In this study, 14 ... more

Y.H. Wang; N. Chen; Y.C. Zhang; C.X. Fu
08/07/2014
Biofuel; Energy crop; Euphorbiaceae; Genetic diversity; Microsatellites; Physic nut

The last few years have seen a significant increase in the number of large-scale sequencing projects generating whole genome databases. These sequence databases can be surveyed (genome sequence survey) for tandem repeats as an alternative means to develop microsatellites for monitoring and selecting natural populations and cultivars of Jatropha curcas. A total of 100 tandem ... more

R.S. Raposo; I.G.B. Souza; M.E.C. Veloso; A.K. Kobayashi; B.G. Laviola; F.M. Diniz
04/03/2014
Animal genetic resources; Genetic management; Microsatellites; mtDNA; Ovis aries

The aim of this study was to analyze genetic diversity and population structure among varieties of White (N = 40), Red (N = 32), and Black (N = 31) Morada Nova hair sheep from flocks in the northeastern Brazilian semiarid region. Fifteen nuclear microsatellite markers and two regions of mitochondrial DNA were used. The intra-population analysis demonstrated that the White variety had ... more

J.S.B. Ferreira; S.R. Paiva; E.C. Silva; C.M. McManus; A.R. Caetano; D.A.E. Façanha; M.A.N. de Sousa
04/27/2015
Endangered species; Microsatellites; Next-generation sequencing; Red fox; Vulpes vulpes

The red fox, Vulpes vulpes (Canidae), is the most widely distributed terrestrial carnivore worldwide, but this species is classified as endangered in Korea. In this study, we developed 25 polymorphic microsatellite markers that included 3-13 (mean = 6.32) alleles per locus using 22 red fox individuals. The most polymorphic locus was FR(59)TG (13 alleles ... more

J.N. Yu; C.U. Chung; K.H. Oh; B.K. Lee; C.E. Lim

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