Research Article

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04/10/2015
DNA; Forensic DNA; Indels; Insertion-deletion polymorphisms

We have previously developed a panel of 40 insertion-deletion (INDEL) human DNA polymorphisms that was proven to ad­equately cover the span of global human genetic diversity. The panel was found to have very low matching probabilities with respect to both the global and Brazilian populations. To optimize the panel for application with degraded DNA samples, which are commonly encountered ... more

V.R.D. Santos; H.B. Pena; S.D.J. Pena
12/13/2013
Mastitis; PCR-PIRA; PCR-RFLP; Percentage of fat; PGLYRP-2 gene; Polymorphism

Peptidoglycan recognition protein 2 (PGLYRP-2), which belongs to the PGRP family, is the only member that has no direct bactericidal activity but has N-acetylmuramoyl-l-alanine amidase activity. This feature of PGLYRP-2 indicates that it may play an important role in eliminating the pathogen associated molecular pattern (PAMP), such as peptidoglycan (PGN), which can reduce ... more

H.L. Wang; Z.X. Li; L. Chen; J. Yang; L.J. Wang; H. He; F.B. Niu; Y. Liu; J.Z. Guo; X.L. Liu
03/16/2012
ATP1A1; Dairy cow; K+-ATPase; Mastitis; Na+; SNP; Somatic cell score

Mastitis affects the concentrations of potassium and sodium in milk. Since sodium-potassium adenosine triphosphatase (Na+, K+-ATPase) is critical for maintaining the homeostasis of these two ions, and is involved in cell apoptosis and pathogenesis, we presumed that polymorphism of the ATP1A1 gene, which encodes the bovine Na+, K+-ATPase ... more

Y.X. Liu; C.H. Xu; T.Y. Gao; Y. Sun
03/30/2007
Brangus; Carcass; DNA; Growth; Growth hormone; SNP

Sequence polymorphisms in the growth hormone (GH) gene and its transcriptional regulators, Pit-1 and Prop-1, were evaluated for associations with growth and carcass traits in two populations of Brangus bulls Chihuahuan Desert Rangeland Research Center (CDRRC, N = 248 from 14 sires) and a cooperating breeding program (COOP, N = 186 from 34 sires). Polymorphisms were SNP mutations in intron 4 (C ... more

M.G. Thomas; R.M. Enns; K.L. Shirley; M.D. Garcia; A.J. Garrett; G.A. Silver
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Acridine orange test; Bovine; Conservation; DNA; Freeze-drying sperm; TUNEL

The ability to detect nuclear damage is an important tool for the development of sperm preservation methods. We used the acridine orange test (AOT) and the terminal deoxynucleotidyl transferase-mediated dUDP nick-end labeling (TUNEL) assay to assess the DNA status of sperm cells preserved with different lyophilization media. The AOT did not detect any differences between different ... more

C.F. Martins; M.N. Dode; S.N. Báo; R. Rumpf
03/10/2009
Algorithm; Bayesian networks; DNA; Paternity testing; Probabilistic expert systems; Software

PedExpert is a Windows-based Bayesian network software, especially constructed to solve problems in parentage testing that are complex because of missing genetic information on the alleged father and/or because they involve genetic mutations. PedExpert automates the creation and manipulation of Bayesian networks, implementing algorithms that convert pedigrees and sets of indispensable ... more

R.R. Gomes; S.V.A. Campos; S.D.J. Pena
04/27/2010
Allele frequency; DNA; Pool; Single nucleotide polymorphism

We examined the efficiency of direct sequencing of pooled DNA for developing common single nucleotide polymorphisms (SNPs) and its accuracy for estimating allele frequencies. A pool of 200 control DNAs was established and was used for developing SNPs and estimating minor allele frequencies (MAF). The sensitivity of the pooled DNA method for successfully detecting an SNP with an MAF >0. ... more

M.K. Kim; T.S. Nam; K.H. Choi; S.Y. Jang; Y.O. Kim; M.C. Lee
03/31/2015
DNA; Mesquite; Mexicali valley; Microorganism; Phosphorous

One of the main limitations in intensive crop production in Northwestern Mexico is the dependence on the use of phosphate fertilizer. In this study, we isolated indigenous microorganisms with phosphate solubilization capacities from mesquite (Prosopis glandulosa) present in the Mexicali valley. In total, 4 bacteria were isolated from the rhizosphere of mesquite, including ICA01, ... more

L. Moreno-Ramírez; D. González-Mendoza; C. Cecena-Duran; O. Grimaldo-Juarez
04/08/2008
Cancer; CDH1 expression; Neuroepithelial tumors; Real-time polymerase chain reaction

Cadherins are cell-to-cell adhesion molecules that play an important role in the establishment of adherent-type junctions by mediating calcium-dependent cellular interactions. The CDH1 gene encodes the transmembrane glycoprotein E-cadherin which is important in maintaining homophilic cell-cell adhesion in epithelial tissues. E-cadherin interacts with catenin proteins to maintain ... more

F.J.N. Motta; E.T. Valera; A.K.B. Lucio-Eterovic; R.G.P. Queiroz; L. Neder; C.A. Scrideli; H.R. Machado; C.G. Carlotti-Junior; S.K.N. Marie; L.G. Tone
01/12/2010
Breast cancer; C3435T polymorphism; MDR1 gene; Multidrug resistance; Real-time polymerase chain reaction

One of the limitations in the treatment of cancer patients with chemotherapy is the development of multidrug resistance (MDR). A well-known mechanism responsible for drug resistance is over-expression of ABC-transporter genes such as MDR1. This gene encodes p-glycoprotein (P-gp), a transmembrane glycoprotein that transports many hydrophobic substrates and anti-cancer drugs out of the cell ... more

M. Taheri; F. Mahjoubi; R. Omranipour

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