Research Article

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11/08/2011
Bovine; cDNA library; Crossbreeds; Expressed sequence tags; Skin

Tick resistance in cattle is mainly found in zebu (Bos indicus) animals, although it is also present in some taurine (B. taurus) breeds. In order to characterize functional genes involved in tick resistance/susceptibility in cattle, two cDNA libraries were generated using skin tissues of selected Holstein x Gyr animals. A total of 2700 high-quality reads from both ... more

C.S. Nascimento; M.A. Machado; S.E.F. Guimarães; M.F. Martins; J.O. Peixoto; J. Furlong; M.C.A. Prata; R.S. Verneque; R.L. Teodoro; P.S. Lopes
11/14/2012
Expressed sequence tags; Mango (Mangifera indica L.); Seed embryo abortion; Subtractive suppression hybridization

Embryo abortion in mango severely damages mango production worldwide. The mechanisms by which the mango embryos abort have long been an intriguing question. We used subtractive suppression hybridization to investigate the differentially expressed genes involved in this process. We generated 2 cDNA libraries from normal seed and aborted seed embryos of mango cultivar ‘Jinhuang’. One ... more

J.H. He; F.W. Ma; Y.Y. Chen; H.R. Shu
03/24/2014
Defense gene; Expressed sequence tags; Melampsora larici-populina; Populus szechuanica; Suppression subtractive hybridization

Poplars are extensively cultivated worldwide, and their susceptibility to the foliar rust fungus leads to considerable damages in plantations. To better understand the molecular basis of poplar responses to the foliar rust, suppression subtractive hybridization was used to identify the potential important or novel genes involved in the Populus szechuanica infection by ... more

Z.J. Chen; Z.M. Cao; Z.D. Yu
06/28/2006
1; 3-b-glucan synthase; Gene structure; Intron evolution; Paracoccidioides brasiliensis

The evolutionary origin and significance of spliceosomal introns have been the subject of many investigations. Two theories, “introns-early” theory and “introns-late” theory, have been proposed to explain the evolution of introns in eukaryotic genes. Intron position is generally conserved in paralogue and orthologue genes. Some introns occur at similar but not necessarily identical positions ... more

M. Pereira; H.T.S. Okamoto; C.M.A. Soares
06/30/2005
Ecology; Hydrolytic enzymes; Paracoccidioides brasiliensis; Saprophytic

Paracoccidioides brasiliensis is a thermally dimorphic fungus that causes paracoccidioidomycosis. The yeast form of this pathogen is found in the animal host whereas the mycelial form is recovered from living and non-living organic material. The sole carbon source available in these habitats is represented by polysaccharides from the plant cell wall. Hydrolytic enzymes are ... more

B. Benoliel; F.B.M. Arraes; V.CasteloBr Reis; S.J.L. de Siqueira; N.S. Parachin; F.A.G. Torres
06/30/2005
Drug targets; Fungi; Paracoccidioides brasiliensis

The rise in antifungal resistance, observed as a result of the increasing numbers of immunocompromised patients, has made the discovery of new targets for drug therapy imperative. The description of the Paracoccidioides brasiliensis transcriptome has allowed us to find alternatives to refine current therapy against paracoccidioidomycosis. We used comparative analysis of ... more

L. Fernandes; A.Sobreira Galdino; M.Sueli Soar Felipe; C.Maria de A. Soares; M. Pereira; A.Corrêa Amaral
06/30/2005
ATP binding cassette transporters; Drug resistance; Major facilitator superfamily; Paracoccidioides brasiliensis

In the struggle for life, the capacity of microorganisms to synthesize and secrete toxic compounds (inhibiting competitors) plays an important role in successful survival of these species. This ability must come together with the capability of being unaffected by these same compounds. Several mechanisms are thought to avoid the toxic effects. One of them is toxin extrusion from the ... more

Cda Silva Costa; F.Caixeta Albuquerque; R.Vieira Andrade; G.Camilo de Oliveira; M.Fernandes de Almeida; Mde Macedo Brigido; Mde Macedo Brigido; A.Queiróz Maranhão; A.Queiróz Maranhão
06/30/2005
Host-fungus interaction; Paracoccidioides brasiliensis; Proteases; Proteasome

Proteases perform a wide variety of functions inside and outside cells, regulating many biological processes. Infectious microorganisms use proteases, either secreted or attached to their cell surface to weaken and invade their hosts. Therefore, proteases are targets for drugs against a diverse set of diseases. Paracoccidioides brasiliensis is the most prevalent fungal ... more

J.Alves Parente; M. Costa; M. Pereira; C.Maria de A. Soares
06/30/2005
Heat shock proteins; Molecular chaperone; Paracoccidioides brasiliensis; Transcriptome

Paracoccidioides brasiliensis is a thermally dimorphic and a human pathogenic fungus. Our group has partially sequenced its transcriptome and generated a database of mycelial and yeast PbAESTs (P. brasiliensis assembled expressed sequence tags). In the present review we describe the identification of PbAESTs encoding molecular chaperones. These proteins, involved in ... more

A.Moraes Nicola; R.Vieira Andrade; I. Silva-Pereira
06/30/2005
Cell wall; GPI-anchored proteins; Paracoccidioides brasiliensis; Plasma membrane

Open reading frames in the transcriptome of Paracoccidioides brasiliensis were screened for potential glycosylphosphatidylinositol (GPI)-anchored proteins, which are a functionally and structurally diverse family of post-translationally modified molecules found in a variety of eukaryotic cells. Numerous studies have demonstrated that various GPI anchor sequences can affect the ... more

Nda Silva Castro; Z.Alves Maia; M. Pereira; C.Maria de A. Soares

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