Research Article

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11/02/2008
cDNA; Expressed sequence tags; heavy metal; woody plant

Many cell functions are redundantly executed in cells, and the experimental approaches that analyze the group of proteins, whose expression is modified in a specific functional condition, enable the identification of the group of proteins that are expressed under stress conditions. The objective of the present study was the evaluation of the genetic expression induced by cadmium (Cd) in ... more

V.L. Souza; A.A.F. Almeida; B.T.Hora Júnior; B.T.Hora Júnior; A.S. Gesteira; J.C.M. Cascardo
11/22/2013
Diversity; Expressed sequence tags; Hevea brasiliensis; Microsatellite

Cultivated clones of Hevea brasiliensis have a narrow genetic base. In order to broaden the genetic base, it is first necessary to investigate the genetic diversity of wild populations. Expressed sequence tag-simple sequence repeat (EST-SSR) markers were developed to investigate the genetic diversity of Hevea populations. Four hundred and thirty microsatellites were ... more

Z.W. An; Y.C. Li; Q.L. Zhai; L.L. Xie; Y.H. Zhao; H.S. Huang
04/17/2013
Expressed sequence tags; Microsatellite; Paeonia delavayi; Population structure

Paeonia Franchet contains approximately 30 species of perennial herbs and is the only member of the family Paeoniaceae. Paeonia delavayi is an endangered ornamental plant that has a compound flower color and is endemic to southwest China. Its optimal habitats have been fragmented and its population size has greatly decreased as a result of human activities. Using a ... more

D.X. Wang; H. Ma; Y.L. Zhang; A.A. Duan; W.J. Li; Z.H. Li
09/17/2012
cDNA library; Expressed sequence tags; Ham; Muscle; Sus scrofa

In general, genetic differences across different breeds of pig lead to variation in mature body size and slaughter age. The Commercial breeds Duroc and Large White and the local Brazilian breed Piau are ostensibly distinct in terms of growth and muscularity, commercial breeds are much leaner while local breeds grow much slower and are fat type pigs. However, the genetic factors that ... more

C.S. Nascimento; J.O. Peixoto; L.L. Verardo; C.F. Campos; M.M.C. Weller; V.R. Faria; M.E. Botelho; M.F. Martins; M.A. Machado; F.F. Silva; P.S. Lopes; S.E.F. Guimarães
10/07/2014
Expressed sequence tags; Genetic characterization; Manila clam; Microsatellites marker; Ruditapes philippinarum

Manila clam (Ruditapes philippinarum) is a valuable and intensively exploited shellfish species in Korea. Despite its importance, information on its genetic background is scarce. For the genetic characterization of R. philippinarum, expressed sequence tag-derived microsatellite markers were developed using next-generation sequencing. A total of 5879 tandem ... more

E.M. Kim; H.S. An; J.H. Kang; C.M. An; C.M. Dong; Y.K. Hong; J.Y. Park
09/26/2014
Amino acid sequences; Expressed sequence tags; Gene expression; In silico analysis

Sweet orange (Citrus sinensis) plays an important role in the economy of more than 140 countries, but it is grown in areas with intermittent stressful soil and climatic conditions. The stress tolerance could be addressed by manipulating the ethylene response factor (ERF) transcription factors because they orchestrate plant responses to environmental stress. We performed an in ... more

T.M. Ito; P.B. Polido; M.C. Rampim; G. Kaschuk; S.G.H. Souza
06/09/2014
Expressed sequence tags; Powdery mildew; Suppression subtraction hybridization; Wheat

Powdery mildew, caused by Blumeria graminis f. sp tritici (Bgt) is one of the devastating diseases of wheat and causes yield losses in temperate wheat growing regions. A wheat line, N0308 with resistance to powdery mildew was used in this study. A suppression subtractive hybridization cDNA library was constructed from the wheat leaves inoculated by Bgt ... more

M.A. Alam; W. Hongpo; Z. Hong; W.Q. Ji
03/24/2014
Expressed sequence tags; Japanese scallop; Mizuhopecten yessoensis; Molecular marker; Simple sequence repeats

Japanese scallop (Mizuhopecten yessoensis) is a cold-water shellfish, and a species of economic importance in China. In this study, we developed and evaluated simple sequence repeat (SSR) markers from the expressed sequence tags (ESTs) of M. yessoensis. The characteristics of 12 EST-SSR loci were investigated in 30 individual scallops, and the result revealed that the ... more

Y.F. Li; X.G. Gao; W.D. Liu; Y. Tian; L. Gao; S.L. Li; C.B. He
03/26/2002
DNA damage; DNA repair; Drug metabolism; Escherichia coli mutagenesis; Oxidative stress; Yeast

The sensitivity responses of seven pso mutants of Saccharomyces cerevisiae towards the mutagens N-nitrosodiethylamine (NDEA), 1,2:7,8-diepoxyoctane (DEO), and 8-hydroxyquinoline (8HQ) further substantiated their allocation into two distinct groups: genes PSO1 (allelic to REV3), PSO2 (SNM1), PSO4 (PRP19), and PSO5 ... more

C. Pungartnik; J. Picada; M. Brendel; J.A.P. Henriques
11/23/2005
Ala16Val polymorphism; APOE gene polymorphism; Atherosclerosis; MnSOD gene polymorphism; ox-LDL; Oxidative damage; Oxidative stress

Oxidized LDL (ox-LDL) is involved in the initiation and progression of atherosclerosis. Many factors can affect the LDL oxidation such as oxidative stress. The present study tested whether ox-LDL levels would be associated with apolipoprotein E (APOE), manganese superoxide dismutase (MnSOD) Ala16Val polymorphisms, and classic cardiovascular risk factors. ox-LDL levels were measured by ... more

M.G.V. Gottlieb; C.H.A. Schwanke; A.F.R. Santos; P.F. Jobim; I.B.M. da Cruz; D.P. Müssel

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