Research Article

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Apoptosis; Brachiaria hybrid; Chromatin fragmentation; Male sterility; Meiosis; Programmed cell death

Morphological changes have been investigated during plant programmed cell death (PCD) in the last few years due to the new interest in a possible apoptotic-like phenomenon existing in plants. Although PCD has been reported in several tissues and specialized cells in plants, there have been few reports of its occurrence during microsporogenesis. The present study reports a typical ... more

V.A. Fuzinatto; M.S. Pagliarini; C.B. Valle
12/05/2011
cDNA-AFLP; Chinese cabbage; Fertility-related genes; Male sterility

To better understand the molecular mechanisms of multiple-allele-inherited male sterility in Chinese cabbage (Brassica rapa ssp pekinensis), differentially expressed genes in fertile and sterile plants must be isolated. We used cDNA-AFLP analysis to isolate differentially expressed genes in fertile and sterile buds of the two-type line, AB01. Sixteen high-quality ... more

R.Q. Ji; Q. Song; X.F. Xin; X. Zhou; H. Feng
09/09/2011
Breeding; Corn silage; Male sterility; Meiotic mutation; ms17

Cytological analysis under light microscopy of the single hybrid P30R50 of silage corn revealed an abnormal pattern of microsporogenesis that affected the meiotic products. Meiosis progressed normally until diakinesis, but before migration to the metaphase plate, bivalents underwent total desynapsis and 20 univalent chromosomes were scattered in the cytoplasm. At this stage, meiocytes ... more

M.S. Pagliarini; V.F. Souza; N. Silva; C.A. Scapim; M. Rodovalho; M.V. Faria
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Fertility restoration; Gene expression; Homologous cloning; Male sterility; YABBY gene family

Expression of the YABBY gene family in the abaxial surface of lateral plant organs determines abaxial destiny of cells, enhances growth and expansion of lateral organs, and plays an important role in polar establishment of lateral organs. However, the YABBY gene has not been studied in male sterility and fertility restoration. We homologously cloned the CtYABBY1 gene of male-sterile TC1 ... more

X.L. Zhang; L.G. Zhang
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3-glucanase; Antagonistic yeast XL-1; Chitinase; Peroxidase; β-1

The effect of the antagonistic yeast XL-1 on resistance-associated enzyme activities in postharvest cantaloupe was studied by inoculating the antagonistic yeast XL-1. Cantaloupes were sterilized, dried in air, and soaked in antagonistic yeast treatment liquid for 30 s. After drying in air, the cantaloupe was stored at room temperature (2°-5°C). The activities of resistance-associated ... more

C.H. Shan; W. Chen; H. Zhang; F.X. Tang; J.M. Tong
06/22/2010
Conservation; MicroRNAs; Target genes; Wheat

MicroRNAs (miRNAs) are non-coding small RNAs that regulate gene expression by translational repression or transcript degradation. A large number of miRNAs have been identified from model plant species; however, the character of conserved miRNAs is poorly understood. We studied 42 miRNA families that are conserved within the plant kingdom, using the miRBase database. Some conserved miRNA ... more

Z.J. Yin; F.F. Shen
10/30/2013
AFLP; Cytoplasmic male sterility; Molecular marker; mtDNA; Wheat

To produce a good F1 hybrid variety wheat crop, it is necessary to explore novel cytoplasmic male sterility (CMS) lines and their maintainer line. This study aimed to identify cytoplasmic variation in three isonuclear-alloplasmic male sterile lines Aegilops kotschyi (Ae.kots) -90-110, Aegilops ventricosa (Ae.ven) -90-110, and Triticum spelta ( ... more

M. Ejaz; Z. Qidi; Z. Gaisheng; W. Qunzhu; Z. Xinbo
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marker-assisted selection; Psathyrostachys huashanica; RAPD; Repetitive sequence; SCAR; Wheat

In this study, we cloned and sequenced a 938-base pair polymorphic band, pHs27, in the tightly linked random amplified polymorphic DNA marker OPU10 and converted it into a sequence-characterized amplified region (SCAR) marker referred to as RHS141, which was specific for the Ns genome of Psathyrostachys huashanica. A GenBank basic local alignment search tool search showed that ... more

W.L. Du; J. Wang; L.M. Wang; Y.H. Pang; J. Wu; J.X. Zhao; Q.H. Yang; X.H. Chen
10/07/2013
MicroRNA; Northern blot; UV-B radiation; Wheat

MicroRNAs (miRNAs) play critical roles in post-transcriptional gene regulation and act as important endogenous regulators to various stressors. Ultraviolet-B (UV-B) radiation is a major factor influencing crop growth and development. In this study, we isolated a novel wheat miRNA, named Tae-miR6000, and confirmed its expression diversity after UV-B treatments. Additionally, using the ... more

B. Wang; Y.F. Sun; N. Song; X.J. Wang; H. Feng; L.L. Huang; Z.S. Kang
09/12/2013
Dwarf mutant; Genetic analysis; Gibberellic acid; SSR analysis; Wheat

A spontaneous dwarf mutant of wheat was found in an F5 generation line derived from a cross between Huamai No. 9 and Een No. 1 in 1998; it was named Huaai 01. We characterized the genetic pattern of Huaai 01 and mapped the gene controlling the dwarf trait. This dwarf mutant was found insensitive to exogenous gibberellic acid treatment, based on the length of the first leaf and ... more

X.J. Jin; D.F. Sun; H.Y. Li; Y. Yang; G.L. Sun

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